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sycp1 Products By Gene Name

7 Results for "sycp1"

NB300-229

SCP1 Antibody - BSA Free

(3 Reviews)

(56 Publications)

0.1 ml

NB300-229

Simple Western: SCP1 Antibody [NB300-229] - Simple Western lane view shows a specific band for SCP1 in 0.5 mg/ml of Human Testis (left) and Mouse Testis (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western Blot: SCP1 Antibody [NB300-229] - Meiotic chromosome spreads from wild type and Fance mutant (MT) primary spermatocytes isolated from the testes at 20 dpp. Each chromatin spread was staged by analyzing SYCP3 (red) and SYCP1 (green), which are axial element and central region components of the synaptonemal complex respectively. Most of the Fance mutant spermatocytes displayed normal synapsis (middle panels); however a sub-set displayed synapsis abnormalities including an association between non-homologous chromosome ends (arrow head) and abnormal SYCP3 structures (arrows). Dearth and Delayed Maturation of Testicular Germ Cells in Fanconi Anemia E Mutant Male Mice. PLoS One (2016)

Immunocytochemistry/Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] - Double immunofluorescence of testicular spread preparations of the adult mice, SYCP3 (green) and SCP1 (red). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32081844/) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: SCP1 Antibody [NB300-229] - Punctate staining of murine SCP1 in mouse ovary using NB300-229.

Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-229] - SCP1 labeled in mouse pachytene preparation (red), using NB300-229. CDK2 staining, near teleomeres, is also present (green).

Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-229] - Spermatocytes cells fixed in PFA. Detected with anti-mouse 594. ICC/IF image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-229] - Mouse spermatozoa. Green: SCP1 staining. ICC/IF image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-229] - Mouse spermatocyte. Red: scp1 colocalized with scp3 (green). ICC/IF image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] - Immunostaining of SCP3/SYCP3 (NB300-231) and SCP1 on adult Cxxc1 het and CKO chromosome spreads. Green, SCP3/SYCP3; orange, SCP1. Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30365547/) licensed under a CC-BY license.

Meiotic progression and chromosome paring in XX, XO and XY oocytes. (B) Pairing of homologous chromosomes in XX, XO and XY oocytes. Images show the immunofluorescence analysis of SYCP3 (white) and SYCP1 (red), and FISH analysis of the X chromosome (green) and Y chromosome (purple). The dashed squares in the merged images are shown at high magnification (right). The numbers of samples showing the phenotype are shown with the total number tested (left). Arrowheads indicate asynapsed bivalents at the end of the chromosomes. Scale bars, 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32214314), licensed under a CC-BY licence.

Meiotic spreads.Meiotic chromosome spreads from wild type and Fance mutant (MT) primary spermatocytes isolated from the testes at 20 dpp. Each chromatin spread was staged by analyzing SYCP3 (red) and SYCP1 (green), which are axial element and central region components of the synaptonemal complex respectively. Most of the Fance mutant spermatocytes displayed normal synapsis (middle panels); however a sub-set displayed synapsis abnormalities including an association between non-homologous chromosome ends (arrow head) and abnormal SYCP3 structures (arrows). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0159800), licensed under a CC-BY licence.

$hIAPP upregulates the HIF1 alpha /PFKFB3 stress pathway. a Summary of the differentially expressed genes of interest after microarray analysis25 performed on RNA isolated from wild type (WT) and hIAPP overexpressing (HIP) rat islets (4.5 months) presented as a fold change over WT. Asterisk represents genes that were differentially expressed in one experiment. b LDHA and MCT1 mRNA levels in HIP versus WT as measured by qRT-PCR. c Lactate production rate (fold change) measured in isolated islets from HIP relative to WT. d Representative Western blot of PFKFB3 and HIF1 alpha protein levels in whole cell extracts and nuclear-enriched fractions of islets from 6 months old WT (3) and HIP (3) rats. GAPDH and PARP were used as loading controls for whole cell extract (WCE) and nuclear extracts, respectively. e Quantification of HIF1 alpha (upper panel) and PFKFB3 (lower panel) in cytoplasmic and nuclear fractions. Data are presented as mean ± SEM, n = 3 independent experiments for (b) and (d), and n = 4 independent experiments for (c). Statistical significance was analyzed by Student t-test (*p < 0.05, **p < 0.01) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31213603), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

PRDX proteins bind to HIF-1 alpha and HIF-2 alpha A. HeLa cells were transfected with an expression vector encoding V5-epitope-tagged PRDX2 (PRDX2-V5) and exposed to 1% O2 for 24 h. Whole cell lysate (WCL) was subject to immunoprecipitation (IP) using anti-HIF-1 alpha antibody or control IgG, followed by immunoblot assays with antibody against V5 epitope or HIF-1 alpha . B. HeLa cells were transfected with PRDX2-V5 vector and exposed to 1% O2 for 24 h. The WCL was subject to IP using anti-V5 antibody or control IgG, followed by immunoblot assays with antibody against V5 or HIF-1 alpha . Light IgG: immunoglobulin light chain from the secondary antibody. C. HeLa cells were transfected with vector encoding a V5-tagged PRDX family member and exposed to 1% O2 for 24 h. WCL was subject to IP using anti-HIF-1 alpha antibody, followed by immunoblot assays with antibody against V5 or HIF-1 alpha . D. HeLa cells were transfected with empty vector (EV) or vector encoding a V5-tagged PRDX family member and exposed to 1% O2 for 24 h. WCL was subject to IP using anti-HIF-2 alpha antibody, followed by immunoblot assays with antibody against V5 or HIF-2 alpha . Image collected and cropped by CiteAb from the following open publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

MRE11 associates with the DS region of OriP.A) DNA affinity purification assays were performed with Raji nuclear extracts and DNA templates derived from DS wt, DS nm-, or delta DS. Input and template bound proteins were analyzed by Western blot with antibodies to TRF2, MRE11, EBNA1, PCNA, and PARP1, as indicated. B) Schematic of template DNA used for DNA affinity purification in A, and their respective protein binding sites. C) ChIP assays to monitor EBNA1, TRF2, and MRE11 binding at OriP wt or OriP nm- in transfected 293 cells. Immunoprecipitated DNA was quantified by real time PCR relative to total input DNA recovered for each transfected cell. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/18040525), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Mu, Rt, Ch, Ma, Pa, Pm, Hu(-)

Species Glossary

Reactivity: Mu, Rt, Ch, Ma, Pa, Pm, Hu(-)

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB300-229AF350
Alexa Fluor 405	NB300-229AF405
Alexa Fluor 488	NB300-229AF488
Alexa Fluor 532	NB300-229AF532
Alexa Fluor 594	NB300-229AF594
Alexa Fluor 647	NB300-229AF647
Alexa Fluor 700	NB300-229AF700
Alexa Fluor 750	NB300-229AF750
Allophycocyanin	NB300-229APC
Biotin	NB300-229B
DyLight 350	NB300-229UV
DyLight 405	NB300-229V
DyLight 488	NB300-229G
DyLight 550	NB300-229R
DyLight 594	NB300-229DL594
DyLight 650	NB300-229C
DyLight 680	NB300-229FR
DyLight 755	NB300-229IR
FITC	NB300-229F
HRP	NB300-229H
Janelia Fluor 549	NB300-229JF549
Janelia Fluor 646	NB300-229JF646
PE	NB300-229PE
PerCP	NB300-229PCP
mFluor Violet 450 SE	NB300-229MFV450
mFluor Violet 500 SE	NB300-229MFV500
mFluor Violet 610 SE	NB300-229MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB300-229SS

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NB300-228

SCP1 Antibody

(13 Publications)

0.1 ml

NB300-228

Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-228] - SCP1 labeled in mouse pachytene preparation (red), using NB 300-228. CDK2 staining, near teleomeres, is also present (green).

Simple Western: SCP1 Antibody [NB300-228] - Lane view shows a specific band for SCP1 in 0.5 mg/ml of Human Testis (left) and Mouse Testis (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB300-228AF350
Alexa Fluor 405	NB300-228AF405
Alexa Fluor 488	NB300-228AF488
Alexa Fluor 532	NB300-228AF532
Alexa Fluor 594	NB300-228AF594
Alexa Fluor 647	NB300-228AF647
Alexa Fluor 700	NB300-228AF700
Alexa Fluor 750	NB300-228AF750
Allophycocyanin	NB300-228APC
Biotin	NB300-228B
DyLight 350	NB300-228UV
DyLight 405	NB300-228V
DyLight 488	NB300-228G
DyLight 550	NB300-228R
DyLight 594	NB300-228DL594
DyLight 650	NB300-228C
DyLight 680	NB300-228FR
DyLight 755	NB300-228IR
HRP	NB300-228H
Janelia Fluor 549	NB300-228JF549
Janelia Fluor 646	NB300-228JF646
PE	NB300-228PE
PerCP	NB300-228PCP
mFluor Violet 450 SE	NB300-228MFV450
mFluor Violet 500 SE	NB300-228MFV500
mFluor Violet 610 SE	NB300-228MFV610

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NB300-228PEP

SCP1 Antibody Blocking Peptide

0.05 mg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NB300-229PEP

SCP1 Antibody Blocking Peptide

0.05 mg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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NBP2-54674

SCP1 Antibody

100 ul

NBP2-54674

Immunohistochemistry-Paraffin: SCP1 Antibody [NBP2-54674] - Staining of human testis shows high expression.

Immunohistochemistry-Paraffin: SCP1 Antibody [NBP2-54674] - Staining of human endometrium shows low expression as expected.

<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SCP1 Antibody [NBP2-54674] - Staining in human testis and endometrium tissues using anti-SYCP1 antibody. Corresponding SYCP1 RNA-seq data are presented for the same tissues.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-95256

SCP1 Antibody - Azide and BSA Free

0.1 ml

NBP2-95256

Western Blot: SCP1 Antibody [NBP2-95256] - Analysis of extracts of mouse testis, using SCP1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-95256-0.02ml

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NBP2-54674PEP

SCP1 Recombinant Protein Antigen

100 ul

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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