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![Western Blot: SF2 Antibody [NBP2-20324] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SF2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SF2-Antibody-Western-Blot-NBP2-20324-img0010.jpg "Western Blot: SF2 Antibody [NBP2-20324] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SF2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: SF2 Antibody [NBP2-20324] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SF2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/SF2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20324-img0005.jpg)
![Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-20324] - Paraffin-embedded mouse duodenum. SF2 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/SF2-Antibody-Immunohistochemistry-Paraffin-NBP2-20324-img0007.jpg)
![Western Blot: SF2 Antibody [NBP2-20324] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/SF2-Antibody-Western-Blot-NBP2-20324-img0002.jpg)
![Western Blot: SF2 Antibody [NBP2-20324] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SF2 antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/SF2-Antibody-Western-Blot-NBP2-20324-img0008.jpg)
![Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-20324] - Antibody detects SFRS1 protein at nucleus on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. Antibody dilution: 1:500.](http://images.novusbio.com/fullsize/SF2-Antibody-Immunohistochemistry-Paraffin-NBP2-20324-img0003.jpg)
![Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-20324] - Antibody detects SFRS1 protein at nucleus on colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. Antibody dilution: 1:500.](http://images.novusbio.com/fullsize/SF2-Antibody-Immunohistochemistry-Paraffin-NBP2-20324-img0004.jpg)
![Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-20324] - Paraffin-embedded mouse skin. SF2 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/SF2-Antibody-Immunohistochemistry-Paraffin-NBP2-20324-img0006.jpg)
![Western Blot: SF2 Antibody (8L3T3) [NBP3-16329] - Western blot analysis of extracts of various cell lines, using SRSF1/SF2 Rabbit mAb (NBP3-16329) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/SF2-Antibody-8L3T3-Western-Blot-NBP3-16329-img0004.jpg "Western Blot: SF2 Antibody (8L3T3) [NBP3-16329] - Western blot analysis of extracts of various cell lines, using SRSF1/SF2 Rabbit mAb (NBP3-16329) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunohistochemistry-Paraffin: SF2 Antibody (8L3T3) [NBP3-16329] - Immunohistochemistry of paraffin-embedded mouse brain using SRSF1/SF2 Rabbit mAb (NBP3-16329) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SF2-Antibody-8L3T3-Immunohistochemistry-Paraffin-NBP3-16329-img0003.jpg)
![Immunohistochemistry-Paraffin: SF2 Antibody (8L3T3) [NBP3-16329] - Immunohistochemistry of paraffin-embedded rat brain using SRSF1/SF2 Rabbit mAb (NBP3-16329) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SF2-Antibody-8L3T3-Immunohistochemistry-Paraffin-NBP3-16329-img0001.jpg)
![Immunohistochemistry-Paraffin: SF2 Antibody (8L3T3) [NBP3-16329] - Immunohistochemistry of paraffin-embedded human colon using SRSF1/SF2 Rabbit mAb (NBP3-16329) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SF2-Antibody-8L3T3-Immunohistochemistry-Paraffin-NBP3-16329-img0002.jpg)
![Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-38912] - Staining of human lymph node shows strong nuclear positivity in germinal and non-germinal center cells.](http://images.novusbio.com/fullsize/SF2-Antibody-Immunohistochemistry-Paraffin-NBP2-38912-img0005.jpg "Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-38912] - Staining of human lymph node shows strong nuclear positivity in germinal and non-germinal center cells.")
![Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-38912] - Staining of human colon shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SF2-Antibody-Immunohistochemistry-Paraffin-NBP2-38912-img0002.jpg)
![Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-38912] - Staining of human cervix, uterine shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/SF2-Antibody-Immunohistochemistry-Paraffin-NBP2-38912-img0003.jpg)
![Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-38912] - Staining of human liver shows strong nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/SF2-Antibody-Immunohistochemistry-Paraffin-NBP2-38912-img0004.jpg)
![Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-38912] - Staining of human rectum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SF2-Antibody-Immunohistochemistry-Paraffin-NBP2-38912-img0006.jpg)
![Western Blot: SF2 Antibody [NBP1-19093] - Whole cell lysate from HeLa and 293T cells. Affinity purified rabbit anti- ASF antibody used for WB. ASF was also immunoprecipitated by rabbit anti-ASF antibody NBP1-19094.](http://images.novusbio.com/fullsize/SF2-Antibody-Western-Blot-NBP1-19093-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: SF2 Antibody [NBP1-19093] - Whole cell lysate from HeLa and 293T cells. Affinity purified rabbit anti- ASF antibody used for WB. ASF was also immunoprecipitated by rabbit anti-ASF antibody NBP1-19094.")
![Western Blot: SF2 Antibody [NBP1-19094] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IPloaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ASF antibody used for WB at 1 ug/ml (A and B) and used for IP at 10 ug/mg lysate.ASF was also immunoprecipitated by rabbit anti-ASF antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposuretimes of 10 seconds (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/SF2-Antibody-Western-Blot-NBP1-19094-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: SF2 Antibody [NBP1-19094] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IPloaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ASF antibody used for WB at 1 ug/ml (A and B) and used for IP at 10 ug/mg lysate.ASF was also immunoprecipitated by rabbit anti-ASF antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposuretimes of 10 seconds (A) and 30 seconds (B).")
![Western Blot: SF2 Overexpression Lysate (Adult Normal) [NBP2-10637] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SF2.](http://images.novusbio.com/fullsize/SF2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10637-img0001.jpg "Western Blot: SF2 Overexpression Lysate (Adult Normal) [NBP2-10637] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SF2.")
![Western Blot: SF2 Overexpression Lysate (Adult Normal) [NBP2-11448] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SF2.](http://images.novusbio.com/fullsize/SF2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11448-img0001.jpg "Western Blot: SF2 Overexpression Lysate (Adult Normal) [NBP2-11448] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SF2.")
![Western Blot: SF2 Overexpression Lysate (Adult Normal) [NBL1-15886] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SF2.](http://images.novusbio.com/fullsize/SF2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15886-img0002.jpg "Western Blot: SF2 Overexpression Lysate (Adult Normal) [NBL1-15886] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SF2.")
![Western Blot: SF2 Antibody [H00006426-B01P] - Analysis of SFRS1 expression in transfected 293T cell line by SFRS1 polyclonal antibody. Lane 1: SFRS1 transfected lysate(27.39 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SF2-Antibody-Western-Blot-H00006426-B01P-img0001.jpg "Western Blot: SF2 Antibody [H00006426-B01P] - Analysis of SFRS1 expression in transfected 293T cell line by SFRS1 polyclonal antibody. Lane 1: SFRS1 transfected lysate(27.39 KDa). Lane 2: Non-transfected lysate.")
![SDS-Page: SF2 Protein [NBP2-53109]](http://images.novusbio.com/fullsize/SF2-Protein-SDS-Page-NBP2-53109-img0001.jpg "SDS-Page: SF2 Protein [NBP2-53109]")
![SDS-Page: Recombinant Human SF2 Protein [NBP2-59579] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-SF2-Protein-SDS-Page-NBP2-59579-img0001.jpg "SDS-Page: Recombinant Human SF2 Protein [NBP2-59579] - 15% SDS-PAGE (3ug)")