Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993] - Analysis in human kidney and pancreas tissues using NBP1-80993 antibody. Corresponding SLC12A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-Paraffin-NBP1-80993-img0018.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993] - Analysis in human kidney and pancreas tissues using NBP1-80993 antibody. Corresponding SLC12A1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993] - Staining of human cerebellum, kidney, liver and pancreas using Anti-SLC12A1 antibody NBP1-80993 (A) shows similar protein distribution across tissues to independent antibody NBP1-82559 (B).](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-Paraffin-NBP1-80993-img0013.jpg)
![Western Blot: NKCC2/SLC12A1 Antibody [NBP1-80993] - Western blot experiments were performed to determine the protein expression in eight corresponding normal (N) and clear cell renal cell carcinoma (T) tissue. NPTX2 was increased in tumor tissue, whereas FXYD4, KNG1, SLC12A1 and SLC6A3 were decreased in tumors. NDUF4AL2 levels were similar, but the bands were of different size in tumor and normal samples Image collected and cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/s12943-016-0495-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Western-Blot-NBP1-80993-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: NKCC2/SLC12A1 Antibody [NBP1-80993] - Staining of human cell line RPTEC TERT1 shows localization to nucleus & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80993-img0007.jpg)
![Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993] - Staining of human cerebellum shows no positivity in Purkinje cells as expected.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-Paraffin-NBP1-80993-img0012.jpg)
![Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993] - Staining of human kidney shows strong membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-Paraffin-NBP1-80993-img0014.jpg)
![Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-Paraffin-NBP1-80993-img0015.jpg)
![Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-Paraffin-NBP1-80993-img0016.jpg)
![Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining in human kidney and pancreas tissues using NBP1-82559 antibody. Corresponding SLC12A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-Paraffin-NBP1-82559-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining in human kidney and pancreas tissues using NBP1-82559 antibody. Corresponding SLC12A1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining of human cerebellum shows no positivity in Purkinje cells as expected.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-Paraffin-NBP1-82559-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining of human cerebellum, kidney, liver and pancreas using Anti-SLC12A1 antibody NBP1-82559 (A) shows similar protein distribution across tissues to independent antibody NBP1-80993 (B).](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-Paraffin-NBP1-82559-img0010.jpg)
![Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining of human kidney shows strong membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-Paraffin-NBP1-82559-img0011.jpg)
![Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-Paraffin-NBP1-82559-img0012.jpg)
![Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-Paraffin-NBP1-82559-img0013.jpg)
![Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP3-12228] - Rat stomach-NBP3-12228. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and hematoxylin QS (blue) counterstrain. 40x magnification on leica DM4000](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-Paraffin-NBP3-12228-img0002.jpg "Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP3-12228] - Rat stomach-NBP3-12228. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and hematoxylin QS (blue) counterstrain. 40x magnification on leica DM4000")
![Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP3-12228] - Rat stomach. 1:150 dilution IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 10X, 40X magnification.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-Paraffin-NBP3-12228-img0001.jpg)
![Western Blot: NKCC2/SLC12A1 Antibody [NBP1-57622] - Sample Tissue: Human A549 Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Western-Blot-NBP1-57622-img0007.jpg "Western Blot: NKCC2/SLC12A1 Antibody [NBP1-57622] - Sample Tissue: Human A549 Antibody Dilution: 1.0 ug/ml")
![Immunohistochemistry: NKCC2/SLC12A1 Antibody [NBP1-57622] - Paraffin Embedded Tissue: Human Kidney Cellular Data: Epithelial cells of renal tubule Antibody Concentration: 4.0 - 8.0 ug/ml Magnification: 400X](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-NBP1-57622-img0006.jpg)
![Western Blot: NKCC2/SLC12A1 Antibody [NBP1-57622] - Jurkat cell lysate, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Western-Blot-NBP1-57622-img0002.jpg)
![Western Blot: NKCC2/SLC12A1 Antibody [NBP1-57622] - Sample Tissue: Jurkat, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane/lane, Gel Concentration: 0.12](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Western-Blot-NBP1-57622-img0005.jpg)
![Western Blot: NKCC2/SLC12A1 Antibody (JE64-26) [NBP3-32951] - Western blot analysis of NKCC2/SLC12A1 on different lysates with Rabbit anti-NKCC2/SLC12A1 antibody (NBP3-32951) at 1/1,000 dilution. Lane 1: mouse kidney tissue lysate (no heat) Lane 2: mouse kidney tissue lysate Lane 3: rat kidney tissue lysate Lane 4: K-562 cell lysate (no heat) Tissue lysates at 30 ug/Lane. Cell lysates at 20 ug/Lane. Predicted band size: 121 kDa Observed band size: 150 kDa Exposure time: 1 minutes 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32951) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32951_rabbit-nkcc2-slc12a1-mab-je64-26-288202412442980.jpg "Western Blot: NKCC2/SLC12A1 Antibody (JE64-26) [NBP3-32951] - Western blot analysis of NKCC2/SLC12A1 on different lysates with Rabbit anti-NKCC2/SLC12A1 antibody (NBP3-32951) at 1/1,000 dilution. Lane 1: mouse kidney tissue lysate (no heat) Lane 2: mouse kidney tissue lysate Lane 3: rat kidney tissue lysate Lane 4: K-562 cell lysate (no heat) Tissue lysates at 30 ug/Lane. Cell lysates at 20 ug/Lane. Predicted band size: 121 kDa Observed band size: 150 kDa Exposure time: 1 minutes 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32951) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NKCC2/SLC12A1 Antibody (JE64-26) [NBP3-32951] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-NKCC2/SLC12A1 antibody (NBP3-32951) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32951) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32951_rabbit-nkcc2-slc12a1-mab-je64-26-298202414333656.jpg)
![Immunocytochemistry/ Immunofluorescence: NKCC2/SLC12A1 Antibody (JE64-26) [NBP3-32951] - Immunocytochemistry analysis of K562 cells labeling NKCC2/SLC12A1 with Rabbit anti-NKCC2/SLC12A1 antibody (NBP3-32951) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde and 80% methanol for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NKCC2/SLC12A1 antibody (NBP3-32951) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32951_rabbit-nkcc2-slc12a1-mab-je64-26-2920241192380.jpg)
![Western Blot: NKCC2/SLC12A1 Antibody [NBP1-44269] - Western blot analysis of Rat kidney tissue lysates showing detection of NKCC2/SLC12A1 protein using Rabbit Anti-NKCC2/SLC12A1 Polyclonal Antibody (NBP1-44269). Primary Antibody: Rabbit Anti-NKCC2/SLC12A1 Polyclonal Antibody (NBP1-44269) at 1:1000.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Western-Blot-NBP1-44269-img0004.jpg "Western Blot: NKCC2/SLC12A1 Antibody [NBP1-44269] - Western blot analysis of Rat kidney tissue lysates showing detection of NKCC2/SLC12A1 protein using Rabbit Anti-NKCC2/SLC12A1 Polyclonal Antibody (NBP1-44269). Primary Antibody: Rabbit Anti-NKCC2/SLC12A1 Polyclonal Antibody (NBP1-44269) at 1:1000.")
![Immunohistochemistry: NKCC2/SLC12A1 Antibody [NBP1-44269] - Immunohistochemistry analysis using Rabbit Anti-NKCC2/SLC12A1 Polyclonal Antibody (NBP1-44269). Tissue: Kidney. Species: Rat. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-NKCC2/SLC12A1 Polyclonal Antibody (NBP1-44269) at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) NKCC2/SLC12A1 Antibody (D) Composite](http://images.novusbio.com/fullsize/NKCC2-SLC12A1 Antibody-Immunohistochemistry-NBP1-44269-img0005.jpg)
![Western Blot: NKCC2/SLC12A1 Antibody (S07-5D1) [NBP3-19717] - Western blot detection of NKCC2/SLC12A1 in Hela cell lysates using NBP3-19717 (1:1000 diluted). Predicted band size: 122kDa. Observed band size: 150kDa.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-S07-5D1-Western-Blot-NBP3-19717-img0001.jpg "Western Blot: NKCC2/SLC12A1 Antibody (S07-5D1) [NBP3-19717] - Western blot detection of NKCC2/SLC12A1 in Hela cell lysates using NBP3-19717 (1:1000 diluted). Predicted band size: 122kDa. Observed band size: 150kDa.")
![SDS-Page: Recombinant Human NKCC2/SLC12A1 Protein [H00006557-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00006557-P01-1.jpg "SDS-Page: Recombinant Human NKCC2/SLC12A1 Protein [H00006557-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: SLC12A1 Overexpression Lysate (Adult Normal) [NBP2-09034] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SLC12A1.](http://images.novusbio.com/fullsize/SLC12A1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09034-img0001.jpg "Western Blot: SLC12A1 Overexpression Lysate (Adult Normal) [NBP2-09034] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SLC12A1.")
![Western Blot: NKCC2/SLC12A1 Antibody (4H4) [H00006557-M03] - Analysis of SLC12A1 expression in transfected 293T cell line by SLC12A1 monoclonal antibody (M03), clone 4H4. Lane 1: SLC12A1 transfected lysatE (46.5 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-4H4-Western-Blot-H00006557-M03-img0002.jpg "Western Blot: NKCC2/SLC12A1 Antibody (4H4) [H00006557-M03] - Analysis of SLC12A1 expression in transfected 293T cell line by SLC12A1 monoclonal antibody (M03), clone 4H4. Lane 1: SLC12A1 transfected lysatE (46.5 KDa). Lane 2: Non-transfected lysate.")