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![Western Blot: RanBP1 Antibody [NB100-79814] - Detection of Human RanBP1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RanBP1 antibody NB100-79814 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RanBP1-Antibody-Western-Blot-NB100-79814-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: RanBP1 Antibody [NB100-79814] - Detection of Human RanBP1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RanBP1 antibody NB100-79814 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: RanBP1 Antibody [NB100-79814] - Detection of Human RanBP1 on HeLa whole cell lysate using NB100-79814. RanBP1 was also immunoprecipitated using rabbit anti-RanBP1 antibody BL1968 NB100-79815 at 5 mcg/0.5 mg lysate.](http://images.novusbio.com/fullsize/RanBP1-Antibody-Western-Blot-NB100-79814-img0002.jpg)
![Western Blot: RanBP1 Antibody [NB100-1443] - Staining of A431 (A) with antibody at 0.03 ug/mL, HeLa (B) with antibody at 0.5 ug/mL and MCF7 (C) cell lysate with antibody at 0.1 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/RanBP1-Antibody-Western-Blot-NB100-1443-img0002.jpg "Western Blot: RanBP1 Antibody [NB100-1443] - Staining of A431 (A) with antibody at 0.03 ug/mL, HeLa (B) with antibody at 0.5 ug/mL and MCF7 (C) cell lysate with antibody at 0.1 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: RanBP1 Antibody [NB100-1443] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/RanBP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1443-img0004.jpg)
![Immunohistochemistry-Paraffin: RanBP1 Antibody [NB100-1443] - Staining of paraffin embedded Human Kidney with antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/RanBP1-Antibody-Immunohistochemistry-Paraffin-NB100-1443-img0006.jpg)
![Flow Cytometry: RanBP1 Antibody [NB100-1443] - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/RanBP1-Antibody-Flow-Cytometry-NB100-1443-img0005.jpg)
![Western Blot: RanBP1 Antibody [NB100-1443] - Staining of Human Duodenum lysate with antibody at 0.3 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/RanBP1-Antibody-Western-Blot-NB100-1443-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: RanBP1 Antibody [NB100-1443] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/RanBP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1443-img0003.jpg)
![Western Blot: RanBP1 Antibody (HL2358) - Azide and BSA Free [NBP3-25621] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RanBP1 antibody [HL2358] (NBP3-25621) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25621_rabbit-ranbp1-mab-hl2358-azide-and-bsa-free-2120249374914.jpg "Western Blot: RanBP1 Antibody (HL2358) - Azide and BSA Free [NBP3-25621] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RanBP1 antibody [HL2358] (NBP3-25621) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Knockout Validated: RanBP1 Antibody (HL2358) - Azide and BSA Free [NBP3-25621] - Wild-type (WT) and RanBP1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RanBP1 antibody [HL2358] (NBP3-25621) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25621_rabbit-ranbp1-mab-hl2358-azide-and-bsa-free-2120249422650.jpg)
![Immunocytochemistry/Immunofluorescence: RanBP1 Antibody (HL2358) - Azide and BSA Free [NBP3-25621] - RanBP1 antibody [HL2358] detects RanBP1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RanBP1 stained by RanBP1 antibody [HL2358] (NBP3-25621) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25621_rabbit-ranbp1-mab-hl2358-azide-and-bsa-free-212024936276.jpg)
![Western Blot: RanBP1 Antibody (HL2358) - Azide and BSA Free [NBP3-25621] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RanBP1 antibody [HL2358] (NBP3-25621) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25621_rabbit-ranbp1-mab-hl2358-azide-and-bsa-free-2120249374910.jpg)
![Immunohistochemistry-Paraffin: RanBP1 Antibody [NBP2-57339] - Staining in human testis and kidney tissues using anti-RANBP1 antibody. Corresponding RANBP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RanBP1-Antibody-Immunohistochemistry-Paraffin-NBP2-57339-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RanBP1 Antibody [NBP2-57339] - Staining in human testis and kidney tissues using anti-RANBP1 antibody. Corresponding RANBP1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: RanBP1 Antibody [NBP2-57339] - Staining of human cell line RH-30 shows localization to cytosol.](http://images.novusbio.com/fullsize/RanBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57339-img0001.jpg)
![Immunohistochemistry-Paraffin: RanBP1 Antibody [NBP2-57339] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/RanBP1-Antibody-Immunohistochemistry-Paraffin-NBP2-57339-img0002.jpg)
![Immunohistochemistry-Paraffin: RanBP1 Antibody [NBP2-57339] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/RanBP1-Antibody-Immunohistochemistry-Paraffin-NBP2-57339-img0003.jpg)
![Western Blot: RanBP1 Antibody [NB100-79815] - Detection of human RanBP1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- RanBP1 (NB100-79815). Detection: DAB](http://images.novusbio.com/fullsize/RanBP1-Antibody-Western-Blot-NB100-79815-img0003.jpg "Western Blot: RanBP1 Antibody [NB100-79815] - Detection of human RanBP1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- RanBP1 (NB100-79815). Detection: DAB")
![Immunoprecipitation: RanBP1 Antibody [NB100-79815] - Detection of human RanBP1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RanBP1 antibody NB100-79815 (lot 2) used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. RanBP1 was also immunoprecipitated by the previous lot (lot 1) of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A and B)](http://images.novusbio.com/fullsize/RanBP1-Antibody-Immunoprecipitation-NB100-79815-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: RanBP1 Antibody [NBP2-57585] - Staining of human cell line RH-30 shows localization to cytosol.](http://images.novusbio.com/fullsize/RanBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57585-img0001.jpg "Immunocytochemistry/Immunofluorescence: RanBP1 Antibody [NBP2-57585] - Staining of human cell line RH-30 shows localization to cytosol.")
![SDS-Page: Recombinant Human RanBP1 Protein [NBP1-99058] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-RanBP1-Protein-SDS-Page-NBP1-99058-img0001.jpg "SDS-Page: Recombinant Human RanBP1 Protein [NBP1-99058] - 15% SDS-PAGE (3ug)")
![SDS-Page: Recombinant Human RanBP1 Protein [NBP1-99058] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-RanBP1-Protein-SDS-Page-NBP1-99058-img0002.jpg)
![Western Blot: RanBP1 Overexpression Lysate (Adult Normal) [NBL1-15139] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RanBP1.](http://images.novusbio.com/fullsize/RanBP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15139-img0002.jpg "Western Blot: RanBP1 Overexpression Lysate (Adult Normal) [NBL1-15139] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RanBP1.")
![Western Blot: RanBP1 Antibody [H00005902-D01P] - Analysis of RANBP1 expression in transfected 293T cell line by RANBP1 polyclonal antibody.Lane 1: RANBP1 transfected lysate(22.11 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RanBP1-Antibody-Western-Blot-H00005902-D01P-img0001.jpg "Western Blot: RanBP1 Antibody [H00005902-D01P] - Analysis of RANBP1 expression in transfected 293T cell line by RANBP1 polyclonal antibody.Lane 1: RANBP1 transfected lysate(22.11 KDa).Lane 2: Non-transfected lysate.")