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![Western Blot: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Protein Kinase D2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Protein Kinase D2.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-5G1-Western-Blot-NBP2-02984-img0015.jpg "Western Blot: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Protein Kinase D2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Protein Kinase D2.")
![Immunocytochemistry/Immunofluorescence: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Protein Kinase D2.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-5G1-Immunocytochemistry-Immunofluorescence-NBP2-02984-img0010.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - Staining of paraffin-embedded Human Kidney tissue using anti-Protein Kinase D2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-5G1-Immunohistochemistry-Paraffin-NBP2-02984-img0014.jpg)
![Flow Cytometry: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Protein Kinase D2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-5G1-Flow-Cytometry-NBP2-02984-img0009.jpg)
![Western Blot: Protein Kinase D2 Antibody (5G1) [NBP2-02984] Analysis of extracts (35ug) from 9 different cell lines by using anti-Protein Kinase D2 monoclonal antibody.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-5G1-Western-Blot-NBP2-02984-img0008.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Protein Kinase D2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-5G1-Immunohistochemistry-Paraffin-NBP2-02984-img0011.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Protein Kinase D2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-5G1-Immunohistochemistry-Paraffin-NBP2-02984-img0012.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Protein Kinase D2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-5G1-Immunohistochemistry-Paraffin-NBP2-02984-img0013.jpg)
![Western Blot: Protein Kinase D2 Antibody [NB100-636] - Detection of Human PKD2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PKD2 antibody NB100-636 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Western-Blot-NB100-636-img0017.jpg "<b>Biological Strategies Validation. </b>Western Blot: Protein Kinase D2 Antibody [NB100-636] - Detection of Human PKD2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PKD2 antibody NB100-636 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Protein Kinase D2 Antibody [NB100-636] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PKD2 antibody (lot For blotting immunoprecipitated PKD2, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Western-Blot-NB100-636-img0015.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Protein Kinase D2 Antibody [NB100-636] - Samples: Whole cell lysate (A. 30 ug/lane; B. 200 mcg/IP) from HEK293 cells that were untreated or transfected with expression constructs for PKD1 and PKD3 as controls for reactivity with these homologs. Antibodies: A. Affinity purified rabbit anti- NB100-636 used at the indicated oncentrations for WB. B. Affinity purified rabbit anti- NB100-636 and NB100-2299 used at 2 ug/200 mcg lysate for IP. Immunoprecipitated PKD2 was detected by WB using anti-PKD2 antibody NB100-2299 at 1 ug/ml. Detection: Chemiluminescence with a 10 second exposure.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Immunoprecipitation-NB100-636-img0003.jpg)
![Western Blot: Protein Kinase D2 Antibody [NBP1-84139] - Analysis using Anti-PRKD2 antibody NBP1-84139 (A) shows similar pattern to independent antibody NBP2-58315 (B).](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Western-Blot-NBP1-84139-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Protein Kinase D2 Antibody [NBP1-84139] - Analysis using Anti-PRKD2 antibody NBP1-84139 (A) shows similar pattern to independent antibody NBP2-58315 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody [NBP1-84139] - Staining in human appendix and cerebral cortex tissues using anti-PRKD2 antibody. Corresponding PRKD2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Immunohistochemistry-Paraffin-NBP1-84139-img0009.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody [NBP1-84139] - Staining of human tonsil shows strong positivity in germinal and non-germinal center cells.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Immunohistochemistry-Paraffin-NBP1-84139-img0006.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody [NBP1-84139] - Staining of human appendix shows high expression.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Immunohistochemistry-Paraffin-NBP1-84139-img0007.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody [NBP1-84139] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Immunohistochemistry-Paraffin-NBP1-84139-img0008.jpg)
![Western Blot: Protein Kinase D2 Antibody [NBP2-99858] - Anti-Protein Kinase D2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 97 kDa. Observed band size: 113 kDa](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Western-Blot-NBP2-99858-img0004.jpg "Western Blot: Protein Kinase D2 Antibody [NBP2-99858] - Anti-Protein Kinase D2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 97 kDa. Observed band size: 113 kDa")
![Immunocytochemistry/Immunofluorescence: Protein Kinase D2 Antibody [NBP2-99858] - Immunofluorescence staining of Protein Kinase D2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Protein Kinase D2 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99858-img0002.jpg)
![Immunoprecipitation: Protein Kinase D2 Antibody [NBP2-99858] - Protein Kinase D2 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate Lane B: 0.5 mg HepG2 Whole Cell Lysate Lane C: 0.5 mg MCF-7 Whole Cell Lysate 4 ul anti-Protein Kinase D2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Protein Kinase D2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 100 kDa. Observed band size: 100 kDa](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Immunoprecipitation-NBP2-99858-img0001.jpg)
![Western Blot: Protein Kinase D2 Antibody [NBP2-58315] - Analysis using Anti-PRKD2 antibody NBP2-58315 (A) shows similar pattern to independent antibody NBP1-84139 (B).](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Western-Blot-NBP2-58315-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Protein Kinase D2 Antibody [NBP2-58315] - Analysis using Anti-PRKD2 antibody NBP2-58315 (A) shows similar pattern to independent antibody NBP1-84139 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Protein Kinase D2 Antibody [NBP2-58315] - Staining of human cell line A549 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58315-img0001.jpg)
![SDS-Page: Recombinant Human Protein Kinase D2 Protein [H00025865-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00025865-P01-1.jpg "SDS-Page: Recombinant Human Protein Kinase D2 Protein [H00025865-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Protein Kinase D2 Overexpression Lysate (Adult Normal) [NBL1-14780] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Protein Kinase D2.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14780-img0002.jpg "Western Blot: Protein Kinase D2 Overexpression Lysate (Adult Normal) [NBL1-14780] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Protein Kinase D2.")
![Western Blot: Protein Kinase D2 Overexpression Lysate (Adult Normal) [NBP2-09273] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Protein Kinase D2.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09273-img0001.jpg "Western Blot: Protein Kinase D2 Overexpression Lysate (Adult Normal) [NBP2-09273] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Protein Kinase D2.")
![Western Blot: Protein Kinase D2 Overexpression Lysate (Adult Normal) [NBP2-09272] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Protein Kinase D2.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09272-img0001.jpg "Western Blot: Protein Kinase D2 Overexpression Lysate (Adult Normal) [NBP2-09272] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Protein Kinase D2.")