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![Western Blot: PDLIM4 Antibody [NBP1-80833] - SH-I-14 induces demethylation and re-expression of tumor suppressor genes. Cells were treated with SH-I-14 for 48 hr and subjected to western blot analysis. The lysates from the cells treated with SH-I-14 for 48 hr were analyzed by western blot with indicated antibodies. beta-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/4054), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PDLIM4-Antibody-Western-Blot-NBP1-80833-img0015.jpg "<b>Biological Strategies Validation. </b>Western Blot: PDLIM4 Antibody [NBP1-80833] - SH-I-14 induces demethylation and re-expression of tumor suppressor genes. Cells were treated with SH-I-14 for 48 hr and subjected to western blot analysis. The lysates from the cells treated with SH-I-14 for 48 hr were analyzed by western blot with indicated antibodies. beta-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/4054), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PDLIM4 Antibody [NBP1-80833] - Staining of human cell line U-2 OS shows localization to actin filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PDLIM4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80833-img0008.jpg)
![Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP1-80833] - Staining of human small intestine shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PDLIM4-Antibody-Immunohistochemistry-Paraffin-NBP1-80833-img0014.jpg)
![Western Blot: PDLIM4 Antibody [NBP1-80833] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/PDLIM4-Antibody-Western-Blot-NBP1-80833-img0003.jpg)
![Western Blot: PDLIM4 Antibody [NBP1-80833] - Analysis in human cell line RH-30.](http://images.novusbio.com/fullsize/PDLIM4-Antibody-Western-Blot-NBP1-80833-img0011.jpg)
![Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP1-80833] - Staining of human Fallopian tube shows weak to moderate membranous and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PDLIM4-Antibody-Immunohistochemistry-Paraffin-NBP1-80833-img0012.jpg)
![Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP1-80833] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/PDLIM4-Antibody-Immunohistochemistry-Paraffin-NBP1-80833-img0013.jpg)
![Western Blot: PDLIM4 Antibody [NBP1-80833] - SH-I-14 reduces the in vivo growth of tumors in xenograft mice bearing tumors of MDA-MB-231 cellsA. SH-I-14 (10 mg/kg) or DMSO was injected into peritoneal cavity of mouse with MDA-MB-231 tumors three times per week from 8-days after tumor cell injection. The control mice were euthanized earlier due to large tumor size. Tumor volumes were measured as described in Materials & Methods. Data are shown as mean±SD. **P < 0.01. B. SH-I-14 reduces acetyl-STAT3 & induces the expression of VHL & PDLIM4 protein in vivo. The MDA-MB-231 xenograft mice (3 mice per group) were administered with 10 mg/kg of SH-I-14 daily for 5 days & lysates from each tumor were subjected to western blot analysis as indicated. beta -actin was used as a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29137356), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-80833_rabbit-polyclonal-pdlim4-antibody-31020241539254.jpg)
![Western Blot: PDLIM4 Antibody [NB100-1382] - (1ug/ml) staining of A431 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence](http://images.novusbio.com/fullsize/PDLIM4-Antibody-Western-Blot-NB100-1382-img0006.jpg "Western Blot: PDLIM4 Antibody [NB100-1382] - (1ug/ml) staining of A431 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence")
![Immunocytochemistry/Immunofluorescence: PDLIM4 Antibody [NB100-1382] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/PDLIM4-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1382-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: PDLIM4 Antibody [NB100-1382] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/PDLIM4-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1382-img0004.jpg)
![Western Blot: PDLIM4 Antibody [NBP2-19759] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PDLIM4-Antibody-Western-Blot-NBP2-19759-img0001.jpg "Western Blot: PDLIM4 Antibody [NBP2-19759] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: PDLIM4 Antibody [NBP2-19759] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PDLIM4 protein stained by PDLIM4 antibody [C2C3], C-term diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/PDLIM4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19759-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PDLIM4 Antibody [NBP2-98063] - Immunofluorescence staining of PDLIM4 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PDLIM4 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/PDLIM4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98063-img0001.jpg "Immunocytochemistry/Immunofluorescence: PDLIM4 Antibody [NBP2-98063] - Immunofluorescence staining of PDLIM4 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PDLIM4 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.")
![Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP2-98063] - Immunochemical staining of human PDLIM4 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PDLIM4-Antibody-Immunohistochemistry-Paraffin-NBP2-98063-img0002.jpg)
![Western Blot: PDLIM4 Antibody [H00008572-B01P] - Analysis of PDLIM4 expression in human spleen.](http://images.novusbio.com/fullsize/PDLIM4-Antibody-Western-Blot-H00008572-B01P-img0002.jpg "Western Blot: PDLIM4 Antibody [H00008572-B01P] - Analysis of PDLIM4 expression in human spleen.")
![Western Blot: PDLIM4 Antibody [H00008572-B01P] - Analysis of PDLIM4 expression in transfected 293T cell line by PDLIM4 polyclonal antibody. Lane 1: PDLIM4 transfected lysate(27.17 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PDLIM4-Antibody-Western-Blot-H00008572-B01P-img0003.jpg)
![Western Blot: PDLIM4 Antibody [NBP2-19758] - Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PDLIM4-Antibody-Western-Blot-NBP2-19758-img0001.jpg "Western Blot: PDLIM4 Antibody [NBP2-19758] - Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: PDLIM4 Overexpression Lysate (Adult Normal) [NBP2-04255] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PDLIM4.](http://images.novusbio.com/fullsize/PDLIM4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04255-img0001.jpg "Western Blot: PDLIM4 Overexpression Lysate (Adult Normal) [NBP2-04255] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PDLIM4.")