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![Western Blot: Nup153 Antibody [NB100-93329] - Knockdown of NUP153 in MDA-MB-231 breast cancer cells. Lane 1: ladder. Lane 2: Scramble Control Lane 3: NUP153 knockdown. Substantially reduced protein detection in knockdown sample and not in scramble control reflect antibody's unique specificity for Nup153. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Nup153-Antibody-Western-Blot-NB100-93329-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Nup153 Antibody [NB100-93329] - Knockdown of NUP153 in MDA-MB-231 breast cancer cells. Lane 1: ladder. Lane 2: Scramble Control Lane 3: NUP153 knockdown. Substantially reduced protein detection in knockdown sample and not in scramble control reflect antibody's unique specificity for Nup153. Image submitted by a verified customer review.")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Nup153 Antibody [NB100-93329] - Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-NUP153 used at a dilution of 1:200 (1ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG highly cross adsorbed Antibody used at a dilution of 1:100.](http://images.novusbio.com/fullsize/Nup153-Antibody-Immunocytochemistry-Immunofluorescence-NB100-93329-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Nup153 Antibody [NB100-93329] - Detection of Human NUP153 on HeLa whole cell lysate using NB100-9329. NUP153 was also immunoprecipitated by rabbit anti-NUP153 antibody NB100-9330.](http://images.novusbio.com/fullsize/Nup153-Antibody-Western-Blot-NB100-93329-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Nup153 Antibody [NBP1-81724] - Staining of human cell line U-251 MG shows localization to nuclear membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Nup153-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81724-img0006.jpg "Immunocytochemistry/Immunofluorescence: Nup153 Antibody [NBP1-81724] - Staining of human cell line U-251 MG shows localization to nuclear membrane. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81724] - Staining of human testis shows strong positivity in nuclear membrane in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Nup153-Antibody-Immunohistochemistry-Paraffin-NBP1-81724-img0010.jpg)
![Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81724] - Staining of human cerebral cortex shows strong positivity in nuclear membrane in neurons.](http://images.novusbio.com/fullsize/Nup153-Antibody-Immunohistochemistry-Paraffin-NBP1-81724-img0007.jpg)
![Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81724] - Staining of human endometrium shows strong positivity in nuclear membrane in glandular cells.](http://images.novusbio.com/fullsize/Nup153-Antibody-Immunohistochemistry-Paraffin-NBP1-81724-img0008.jpg)
![Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81724] - Staining of human lymphoid tissues shows strong positivity in nuclear membrane in germinal center cells and non-germinal center cells.](http://images.novusbio.com/fullsize/Nup153-Antibody-Immunohistochemistry-Paraffin-NBP1-81724-img0009.jpg)
![Western Blot: Nup153 Antibody [NBP1-81725] - Analysis in human cell line K562.](http://images.novusbio.com/fullsize/Nup153-Antibody-Western-Blot-NBP1-81725-img0006.jpg "Western Blot: Nup153 Antibody [NBP1-81725] - Analysis in human cell line K562.")
![Immunocytochemistry/Immunofluorescence: Nup153 Antibody [NBP1-81725] - Staining of human cell line U-251 MG shows localization to nuclear membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Nup153-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81725-img0011.jpg)
![Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81725] - Staining of human testis shows strong positivity in nuclear membrane in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Nup153-Antibody-Immunohistochemistry-Paraffin-NBP1-81725-img0016.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81725] - Analysis in human testis and pancreas tissues. Corresponding NUP153 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Nup153-Antibody-Immunohistochemistry-Paraffin-NBP1-81725-img0012.jpg)
![Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81725] - Staining of human endometrium shows strong positivity in nuclear membrane in glandular cells.](http://images.novusbio.com/fullsize/Nup153-Antibody-Immunohistochemistry-Paraffin-NBP1-81725-img0013.jpg)
![Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81725] - Staining of human pancreas shows weak positivity in nuclear membrane in islets of Langerhans.](http://images.novusbio.com/fullsize/Nup153-Antibody-Immunohistochemistry-Paraffin-NBP1-81725-img0014.jpg)
![Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81725] - Staining of human prostate shows moderate positivity in nuclear membrane and cytoplasm in smooth muscle cells.](http://images.novusbio.com/fullsize/Nup153-Antibody-Immunohistochemistry-Paraffin-NBP1-81725-img0015.jpg)
![Western Blot: Nup153 Antibody (3I5V3) [NBP3-16136] - Western blot analysis of extracts of various cell lines, using Nup153 Rabbit mAb (NBP3-16136) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Nup153-Antibody-3I5V3-Western-Blot-NBP3-16136-img0005.jpg "Western Blot: Nup153 Antibody (3I5V3) [NBP3-16136] - Western blot analysis of extracts of various cell lines, using Nup153 Rabbit mAb (NBP3-16136) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: Nup153 Antibody (3I5V3) [NBP3-16136] - Immunofluorescence analysis of U-2 OS cells using Nup153 Rabbit mAb (NBP3-16136) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Nup153-Antibody-3I5V3-Immunocytochemistry-Immunofluorescence-NBP3-16136-img0004.jpg)
![Immunohistochemistry-Paraffin: Nup153 Antibody (3I5V3) [NBP3-16136] - Immunohistochemistry of paraffin-embedded mouse testis using Nup153 Rabbit mAb (NBP3-16136) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Nup153-Antibody-3I5V3-Immunohistochemistry-Paraffin-NBP3-16136-img0003.jpg)
![Immunohistochemistry-Paraffin: Nup153 Antibody (3I5V3) [NBP3-16136] - Immunohistochemistry of paraffin-embedded rat testis using Nup153 Rabbit mAb (NBP3-16136) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Nup153-Antibody-3I5V3-Immunohistochemistry-Paraffin-NBP3-16136-img0001.jpg)
![Immunohistochemistry-Paraffin: Nup153 Antibody (3I5V3) [NBP3-16136] - Immunohistochemistry of paraffin-embedded human colon using Nup153 Rabbit mAb (NBP3-16136) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Nup153-Antibody-3I5V3-Immunohistochemistry-Paraffin-NBP3-16136-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Nup153 Antibody [NBP1-81723] - Staining of human cell line A-431 shows localization to nuclear membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Nup153-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81723-img0004.jpg "Immunocytochemistry/Immunofluorescence: Nup153 Antibody [NBP1-81723] - Staining of human cell line A-431 shows localization to nuclear membrane. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81723] - Staining of human endometrium shows weak to moderate positivity in nuclear membrane in glandular cells.](http://images.novusbio.com/fullsize/Nup153-Antibody-Immunohistochemistry-Paraffin-NBP1-81723-img0007.jpg)
![Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81723] - Staining of human cerebral cortex shows moderate to strong positivity in nuclear membrane in neurons.](http://images.novusbio.com/fullsize/Nup153-Antibody-Immunohistochemistry-Paraffin-NBP1-81723-img0005.jpg)
![Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81723] - Staining of human testis shows moderate positivity in nuclear membrane in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Nup153-Antibody-Immunohistochemistry-Paraffin-NBP1-81723-img0006.jpg)
![Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81723] - Staining of human pancreas shows very weak positivity in nuclear membrane in exocrine glandular cells.](http://images.novusbio.com/fullsize/Nup153-Antibody-Immunohistochemistry-Paraffin-NBP1-81723-img0008.jpg)
![Immunohistochemistry: Nup153 Antibody (8D4) [NBP3-26450] - Image of Nup153 Antibody (8D4) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26450_rabbit-nup153-mab-8d4-2722024845327.jpg "Immunohistochemistry: Nup153 Antibody (8D4) [NBP3-26450] - Image of Nup153 Antibody (8D4) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunocytochemistry/Immunofluorescence: Nup153 Antibody (8D4) [NBP3-26450] - Staining of Hela Cells with Nup153 Antibody (8D4) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26450_rabbit-nup153-mab-8d4-282202412475127.jpg)
![Western Blot: Nup153 Antibody [NB100-93330] - Detection of Human NUP153 on HeLa whole cell lysate using NB100-93330. NUP153 was also immunoprecipitated by rabbit anti-NUP153 antibody NB100-93329.](http://images.novusbio.com/fullsize/Nup153-Antibody-Western-Blot-NB100-93330-img0004.jpg "Western Blot: Nup153 Antibody [NB100-93330] - Detection of Human NUP153 on HeLa whole cell lysate using NB100-93330. NUP153 was also immunoprecipitated by rabbit anti-NUP153 antibody NB100-93329.")
