Hu,
Mu,
Rt,
Po,
Bv,
Ca,
Ch,
ChHa,
Eq,
Gp,
Ma-Op,
Pm,
Rb,
RM,
ZeSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, ChHa, Eq, Gp, Ma-Op, Pm, Rb, RM, Ze
Applications: WB, IHC, KD
Host: Rabbit Polyclonal
Alt Catalog# IMG-6817A
Sample Size Available
Sizes: Sample Size Available
![Western Blot: LRH-1/NR5A2 Antibody [NBP2-27196] - Analysis of NR5A2 in HEPG2 lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse NIH3T3 and 4) RAW lysate probed with 3 ug/ml of NR5A2 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/LRH-1-NR5A2-Antibody-Western-Blot-NBP2-27196-img0004.jpg "Western Blot: LRH-1/NR5A2 Antibody [NBP2-27196] - Analysis of NR5A2 in HEPG2 lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse NIH3T3 and 4) RAW lysate probed with 3 ug/ml of NR5A2 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: LRH-1/NR5A2 Antibody [NBP2-27196] - Analysis of NR5A2/LRH1 in human pancreas tissue using NR5A2/LRH1 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/LRH-1-NR5A2-Antibody-Immunohistochemistry-Paraffin-NBP2-27196-img0003.jpg)
![Western Blot: LRH-1/NR5A2 Antibody [NBP2-27204] - Analysis of Lrh-1. HEPG2 lysate in the 1) absence and 2) presence of immunizing peptide, 3) RAW lysate probed with 2 ug/ml of Lrh-1 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/LRH-1-NR5A2-Antibody-Western-Blot-NBP2-27204-img0003.jpg "Western Blot: LRH-1/NR5A2 Antibody [NBP2-27204] - Analysis of Lrh-1. HEPG2 lysate in the 1) absence and 2) presence of immunizing peptide, 3) RAW lysate probed with 2 ug/ml of Lrh-1 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Simple Western: LRH-1/NR5A2 Antibody [NBP2-27204] - Simple Western lane view shows a specific band for LRH-1/NR5A2 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LRH-1-NR5A2-Antibody-Simple-Western-NBP2-27204-img0002.jpg)
![Western Blot: LRH-1/NR5A2 Antibody (3H8) [NBP2-46248] - Human recombinant protein fragment corresponding to amino acids 1-303 of human NR5A2(NP_995582) produced in E.coli.](http://images.novusbio.com/fullsize/LRH-1-NR5A2-Antibody-3H8-Western-Blot-NBP2-46248-img0002.jpg "Western Blot: LRH-1/NR5A2 Antibody (3H8) [NBP2-46248] - Human recombinant protein fragment corresponding to amino acids 1-303 of human NR5A2(NP_995582) produced in E.coli.")
![Immunohistochemistry: LRH-1/NR5A2 Antibody (3H8) [NBP2-46248] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/LRH-1-NR5A2-Antibody-3H8-Immunohistochemistry-NBP2-46248-img0001.jpg)
![Western Blot: LRH-1/NR5A2 Antibody [NBP1-32489] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NR5A2 antibody [N2C3] diluted at 1:500.](http://images.novusbio.com/fullsize/LRH-1-NR5A2-Antibody-Western-Blot-NBP1-32489-img0007.jpg "Western Blot: LRH-1/NR5A2 Antibody [NBP1-32489] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NR5A2 antibody [N2C3] diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: LRH-1/NR5A2 Antibody [NBP1-32489] - Paraformaldehyde-fixed Human ESC, using NR5A2 antibody at 1:400 dilution.](http://images.novusbio.com/fullsize/LRH-1-NR5A2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32489-img0004.jpg)
![Western Blot: LRH-1/NR5A2 Antibody (OTI3H8) - Azide and BSA Free [NBP2-72521] - Human recombinant protein fragment corresponding to amino acids 1-303 of human NR5A2(NP_995582) produced in E.coli.](http://images.novusbio.com/fullsize/LRH-1-NR5A2-Antibody-OTI3H8-Azide-and-BSA-Free-Western-Blot-NBP2-72521-img0001.jpg "Western Blot: LRH-1/NR5A2 Antibody (OTI3H8) - Azide and BSA Free [NBP2-72521] - Human recombinant protein fragment corresponding to amino acids 1-303 of human NR5A2(NP_995582) produced in E.coli.")
![Immunohistochemistry: LRH-1/NR5A2 Antibody (OTI3H8) - Azide and BSA Free [NBP2-72521] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/LRH-1-NR5A2-Antibody-OTI3H8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72521-img0002.jpg)
![Western Blot: LRH-1/NR5A2 Antibody [NBP2-42513] - Staining of Pig Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/LRH-1-NR5A2-Antibody-Western-Blot-NBP2-42513-img0004.jpg "Western Blot: LRH-1/NR5A2 Antibody [NBP2-42513] - Staining of Pig Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: LRH-1/NR5A2 Antibody [NBP2-42513] - Analysis of HepG2 nuclear lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/LRH-1-NR5A2-Antibody-Western-Blot-NBP2-42513-img0002.jpg)
![Recombinant Human LRH-1/NR5A2 Protein [H00002494-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00002494-P01-1.jpg "Recombinant Human LRH-1/NR5A2 Protein [H00002494-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: LRH-1/NR5A2 Antibody (SR2100) [NBP3-21744] - Western blot analysis using NBP3-21744 in MCF7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21744_rabbit-lrh-1-nr5a2-mab-sr2100-5720231531238.jpg "Western Blot: LRH-1/NR5A2 Antibody (SR2100) [NBP3-21744] - Western blot analysis using NBP3-21744 in MCF7 cell lysate.")
![Western Blot: LRH-1/NR5A2 Antibody (JE30-50) [NBP3-32660] - Western blot analysis of LRH-1/NR5A2 on different lysates with Rabbit anti-LRH-1/NR5A2 antibody (NBP3-32660) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (15 ug/Lane) Lane 2: 293T cell lysate (15 ug/Lane) Lane 3: HepG2 cell lysate (15 ug/Lane) Lane 4: A549 cell lysate (15 ug/Lane) Lane 5: PANC-1 cell lysate (15 ug/Lane) Lane 6: SW1990 cell lysate (15 ug/Lane) Lane 7: Mouse liver tissue lysate (30 ug/Lane) Lane 8: Rat liver tissue lysate (30 ug/Lane) Lane 9: Mouse brain tissue lysate (30 ug/Lane) Lane 10: Rat brain tissue lysate (30 ug/Lane) Lane 11: Mouse pancreas tissue lysate (30 ug/Lane) Predicted band size: 61 kDa Observed band size: 65 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32660) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32660_rabbit-lrh-1-nr5a2-mab-je30-50-278202416331231.jpg "Western Blot: LRH-1/NR5A2 Antibody (JE30-50) [NBP3-32660] - Western blot analysis of LRH-1/NR5A2 on different lysates with Rabbit anti-LRH-1/NR5A2 antibody (NBP3-32660) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (15 ug/Lane) Lane 2: 293T cell lysate (15 ug/Lane) Lane 3: HepG2 cell lysate (15 ug/Lane) Lane 4: A549 cell lysate (15 ug/Lane) Lane 5: PANC-1 cell lysate (15 ug/Lane) Lane 6: SW1990 cell lysate (15 ug/Lane) Lane 7: Mouse liver tissue lysate (30 ug/Lane) Lane 8: Rat liver tissue lysate (30 ug/Lane) Lane 9: Mouse brain tissue lysate (30 ug/Lane) Lane 10: Rat brain tissue lysate (30 ug/Lane) Lane 11: Mouse pancreas tissue lysate (30 ug/Lane) Predicted band size: 61 kDa Observed band size: 65 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32660) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Western Blot: NR5A2/LRH1 Overexpression Lysate (Adult Normal) [NBP2-04258] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NR5A2/LRH1.](http://images.novusbio.com/fullsize/NR5A2-LRH1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04258-img0001.jpg "Western Blot: NR5A2/LRH1 Overexpression Lysate (Adult Normal) [NBP2-04258] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NR5A2/LRH1.")