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![Western Blot: Menin Antibody [NB100-215] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Menin antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Menin-Antibody-Western-Blot-NB100-215-img0009.jpg "Western Blot: Menin Antibody [NB100-215] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Menin antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin: Menin Antibody [NB100-215] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Menin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/Menin-Antibody-Immunohistochemistry-Paraffin-NB100-215-img0010.jpg)
![Western Blot: Menin Antibody [NB100-215] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Menin antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Menin-Antibody-Western-Blot-NB100-215-img0008.jpg)
![Immunoprecipitation: Menin Antibody [NB100-215] - Detection of human Menin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Menin antibody NB100-215 (lot 2) used for IP at 6 ug per reaction. Menin was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated Menin, NB100-215 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/Menin-Antibody-Immunoprecipitation-NB100-215-img0007.jpg)
![Western Blot: Menin Antibody (7D3E10) [NBP2-37251] - Analysis using MEN1 mAb against HEK293 (1) and MEN1 (AA: 392-554)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Menin-Antibody-7D3E10-Western-Blot-NBP2-37251-img0003.jpg "Western Blot: Menin Antibody (7D3E10) [NBP2-37251] - Analysis using MEN1 mAb against HEK293 (1) and MEN1 (AA: 392-554)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: Menin Antibody (7D3E10) [NBP2-37251] - Analysis of rectum cancer tissues using MEN1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Menin-Antibody-7D3E10-Immunohistochemistry-NBP2-37251-img0005.jpg)
![Flow Cytometry: Menin Antibody (7D3E10) [NBP2-37251] - Analysis of Hela cells using MEN1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Menin-Antibody-7D3E10-Flow-Cytometry-NBP2-37251-img0004.jpg)
![Western Blot: Menin Antibody (7D3E10) [NBP2-37251] - Analysis using MEN1 mAb against human MEN1 (AA: 392-554) recombinant protein. (Expected MW is 43.3 kDa)](http://images.novusbio.com/fullsize/Menin-Antibody-7D3E10-Western-Blot-NBP2-37251-img0002.jpg)
![ELISA: Menin Antibody (7D3E10) [NBP2-37251] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Menin-Antibody-7D3E10-ELISA-NBP2-37251-img0001.jpg)
![Western Blot: Menin Antibody [NB100-287] - Using the Biotin direct conjugate Samples: Whole cell lysate from HEK 293T (50, 15, and 5 ug). Antibody: Affinity purified, biotin-conjugated, goat anti-Menin antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Menin-Antibody-Western-Blot-NB100-287-img0092.jpg "Western Blot: Menin Antibody [NB100-287] - Using the Biotin direct conjugate Samples: Whole cell lysate from HEK 293T (50, 15, and 5 ug). Antibody: Affinity purified, biotin-conjugated, goat anti-Menin antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: Menin Antibody [NB100-287] - Samples: Whole cell lysate (40 ug for WB; one 10 cm plate 293T cells for IP) from 293T cells. Affinity purified goat anti-Menin used as indicated for IP. Menin was detected by Western Blot using affinity purified rabbit anti-Menin NB100-215 at 0.1 ug/ml for WB. Detection: Chemiluminescence exposed for 1 minute.](http://images.novusbio.com/fullsize/Menin-Antibody-Western-Blot-NB100-287-img0003.jpg)
![Western Blot: Menin Antibody [NB100-287] - Analysis using the Biotin conjugate of NB100-287. Detection of HEK 293T (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Menin-Antibody-Western-Blot-NB100-287-img0090.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Menin Antibody [NB100-287] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Menin antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Menin-Antibody-Western-Blot-NB100-287-img0091.jpg)
![Western Blot: Menin Antibody [NB100-399] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Menin antibody (lot was used at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Menin-Antibody-Western-Blot-NB100-399-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Menin Antibody [NB100-399] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Menin antibody (lot was used at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Menin Antibody [NB100-399] - A. Whole cell lysate (40 mcg - E; 10 mcg - T) from 293T cells untreated (E) or transfected with a Menin expression construct (T). B. Whole cell lysate from one 10 cm plate of untreated 293T cells. A. Affinity purified rabbit anti-Menin antibody NB100-399 used at the indicated concentrations for WB. B. Affinity purified rabbit anti-Menin antibodies NB100-215 (5 mcg/plate), NB100-398(10 mcg/plate). Immunoprecipitated Menin was detected using NB100-215 at 0.1 mcg/ml.](http://images.novusbio.com/fullsize/Menin-Antibody-Western-Blot-NB100-399-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Menin Antibody [NB100-399] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Menin antibody used for WB at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Menin-Antibody-Western-Blot-NB100-399-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Menin Antibody [NBP1-90357] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/Menin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90357-img0008.jpg "Immunocytochemistry/Immunofluorescence: Menin Antibody [NBP1-90357] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.")
![Immunohistochemistry-Paraffin: Menin Antibody [NBP1-90357] - Staining of human cerebral cortex shows strong nuclear positivity in glial cells.](http://images.novusbio.com/fullsize/Menin-Antibody-Immunohistochemistry-Paraffin-NBP1-90357-img0007.jpg)
![Western Blot: Menin Antibody (5M7L9) [NBP3-16180] - Western blot analysis of extracts of various cell lines, using Menin Rabbit mAb (NBP3-16180) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Menin-Antibody-5M7L9-Western-Blot-NBP3-16180-img0001.jpg "Western Blot: Menin Antibody (5M7L9) [NBP3-16180] - Western blot analysis of extracts of various cell lines, using Menin Rabbit mAb (NBP3-16180) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: Menin Antibody (5M7L9) [NBP3-16180] - Western blot analysis of extracts of various cell lines, using Menin Rabbit mAb (NBP3-16180) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Menin-Antibody-5M7L9-Western-Blot-NBP3-16180-img0002.jpg)
![Western Blot: Menin Antibody (SR2123) [NBP3-21766] - Western blot analysis using NBP3-21766 in 293T cell lysate.](http://images.novusbio.com/fullsize/nbp3-21766_rabbit-menin-mab-sr2123-57202316582195.jpg "Western Blot: Menin Antibody (SR2123) [NBP3-21766] - Western blot analysis using NBP3-21766 in 293T cell lysate.")
![Western Blot: Menin Antibody (JE30-97) [NBP3-32588] - Western blot analysis of Menin on different lysates with Rabbit anti-Menin antibody (NBP3-32588) at 1/500 dilution. Lane 1: 293 cell lysate Lane 2: PC-3M cell lysate Lane 3: Jurkat cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32588) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32588_rabbit-menin-mab-je30-97-27820241631650.png "Western Blot: Menin Antibody (JE30-97) [NBP3-32588] - Western blot analysis of Menin on different lysates with Rabbit anti-Menin antibody (NBP3-32588) at 1/500 dilution. Lane 1: 293 cell lysate Lane 2: PC-3M cell lysate Lane 3: Jurkat cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32588) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Menin Antibody (JE30-97) [NBP3-32588] - Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-Menin antibody (NBP3-32588) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32588) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32588_rabbit-menin-mab-je30-97-298202413585580.jpg)
![Western Blot: Menin Antibody [NBP2-17277] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/Menin-Antibody-Western-Blot-NBP2-17277-img0001.jpg "Western Blot: Menin Antibody [NBP2-17277] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE gel, diluted at 1:500.")
![Immunoprecipitation: Menin Antibody [NBP2-17277] - Immunoprecipitation of MEN1 protein from 293T whole cell extracts using 5 ug of Menin antibody [N1C1]. Western blot analysis was performed using Menin antibody [N1C1]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Menin-Antibody-Immunoprecipitation-NBP2-17277-img0002.jpg)
![SDS-Page: Recombinant Human Menin Protein [H00004221-P02] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00004221-P02-1.jpg "SDS-Page: Recombinant Human Menin Protein [H00004221-P02] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![SDS-Page: Recombinant Human Menin Protein [H00004221-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00004221-Q01-1.jpg "SDS-Page: Recombinant Human Menin Protein [H00004221-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")