![Western Blot: Menin Antibody [NB100-399] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Menin antibody (lot was used at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Menin-Antibody-Western-Blot-NB100-399-img0004.jpg "Western Blot: Menin Antibody [NB100-399] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Menin antibody (lot was used at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | A synthetic peptide, which represented a portion of human Multiple Endocrine Neoplasia 1 encoded within exon 11 (MEN1 LocusLink ID 4221). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MEN1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | This antibody may be used for Western Blot, where a doublet band should be seen at ~72 kDa. Suggested working dilutions: * Western Blot - 1:500-1:5,000 Immunoprecipitation - 2 to 10 ug/mg lysate Immunofluorescence - ND *The investigator should determine the optimal working dilution for a specific application. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Menin Antibody (NB100-399)Find related products by research area.
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![Western Blot: Menin Antibody [NB100-399] - A. Whole cell lysate (40 mcg - E; 10 mcg - T) from 293T cells untreated (E) or transfected with a Menin expression construct (T). B. Whole cell lysate from one 10 cm plate of untreated 293T cells. A. Affinity purified rabbit anti-Menin antibody NB100-399 used at the indicated concentrations for WB. B. Affinity purified rabbit anti-Menin antibodies NB100-215 (5 mcg/plate), NB100-398(10 mcg/plate). Immunoprecipitated Menin was detected using NB100-215 at 0.1 mcg/ml.](http://images.novusbio.com/fullsize/Menin-Antibody-Western-Blot-NB100-399-img0002.jpg "Western Blot: Menin Antibody [NB100-399] - A. Whole cell lysate (40 mcg - E; 10 mcg - T) from 293T cells untreated (E) or transfected with a Menin expression construct (T). B. Whole cell lysate from one 10 cm plate of untreated 293T cells. A. Affinity purified rabbit anti-Menin antibody NB100-399 used at the indicated concentrations for WB. B. Affinity purified rabbit anti-Menin antibodies NB100-215 (5 mcg/plate), NB100-398(10 mcg/plate). Immunoprecipitated Menin was detected using NB100-215 at 0.1 mcg/ml.")
![Western Blot: Menin Antibody [NB100-399] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Menin antibody used for WB at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Menin-Antibody-Western-Blot-NB100-399-img0003.jpg "Western Blot: Menin Antibody [NB100-399] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Menin antibody used for WB at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
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