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![Western Blot: MED12 Antibody [NB100-2357] - Components from all modules of Mediator are found in TAP-wtICP4 samples. Western blot analysis of nuclear extracts (NE) or affinity purified (PD) samples using either wtICP4 (KOS) or TAP-wtICP4 (TAP) expressing viruses at 6 hpi. Results of the Western blot analysis for components of the head (Med6), middle (Med4 and Med1), tail (Med23), kinase (cdk8, Med12, and Med13), and the Med26 protein are shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0078242), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MED12-Antibody-Western-Blot-NB100-2357-img0015.jpg "Western Blot: MED12 Antibody [NB100-2357] - Components from all modules of Mediator are found in TAP-wtICP4 samples. Western blot analysis of nuclear extracts (NE) or affinity purified (PD) samples using either wtICP4 (KOS) or TAP-wtICP4 (TAP) expressing viruses at 6 hpi. Results of the Western blot analysis for components of the head (Med6), middle (Med4 and Med1), tail (Med23), kinase (cdk8, Med12, and Med13), and the Med26 protein are shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0078242), licensed under a CC-BY license.")
![Immunohistochemistry: MED12 Antibody [NB100-2357] - Representative examples of immunohistochemically stained sections positive for MED12 (A, B and C) in tumor specimens. Examples are from the same patients, respectively. A. TS = 5, B. TS = 6, C. TS = 6 (x20 original magnification [A, B and C]. Scale bars represent 100 um. TS, total score = intensity score (0-3) plus proportion score (0-3). Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/abstract/14410), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MED12-Antibody-Immunohistochemistry-NB100-2357-img0014.jpg)
![Western Blot: MED12 Antibody [NB100-2357] - Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Med12 antibody (NB100-2357 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nb100-2357_rabbit-polyclonal-med12-antibody-4122023957332.jpg)
![Western Blot: MED12 Antibody [NB100-2357] - Samples: Whole cell lysate (10 ug) from HeLa, A-549, GaMG, Hep-G2, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Med12 antibody (NB100-2357 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nb100-2357_rabbit-polyclonal-med12-antibody-4122023957333.jpg)
![Immunohistochemistry-Paraffin: MED12 Antibody [NB100-2357] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MED12 (NB100-2357 Lot 3) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/nb100-2357_rabbit-polyclonal-med12-antibody-4122023957330.jpg)
![Western Blot: MED12 Antibody [NB100-2357] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Med12 antibody (NB100-2357 lot 3) used for IP at 6 ug per reaction. Med12 was also immunoprecipitated by a previous lot of this antibody (NB100-2357 lot 2) and a second antibody against a different epitope of Med12 (NBP2-80138). For blotting immunoprecipitated Med12, NB100-2357 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/nb100-2357_rabbit-polyclonal-med12-antibody-4122023957334.jpg)
![Immunohistochemistry-Paraffin: MED12 Antibody [NB100-2357] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-MED12 (NB100-2357 Lot 3) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/nb100-2357_rabbit-polyclonal-med12-antibody-4122023957331.jpg)
![Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669] - Staining in human placenta and pancreas tissues using anti-MED12 antibody. Corresponding MED12 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MED12-Antibody-Immunohistochemistry-Paraffin-NBP1-86669-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669] - Staining in human placenta and pancreas tissues using anti-MED12 antibody. Corresponding MED12 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669] - Staining of human cerebral cortex, colon, spleen and testis using Anti-MED12 antibody NBP1-86669 (A) shows similar protein distribution across tissues to independent antibody NBP1-86670 (B).](http://images.novusbio.com/fullsize/MED12-Antibody-Immunohistochemistry-Paraffin-NBP1-86669-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MED12 Antibody [NBP1-86669] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-MED12 antibody. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize/MED12-Antibody-Western-Blot-NBP1-86669-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: MED12 Antibody [NBP1-86669] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MED12-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86669-img0009.jpg)
![Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669] - Staining of human colon.](http://images.novusbio.com/fullsize/MED12-Antibody-Immunohistochemistry-Paraffin-NBP1-86669-img0015.jpg)
![Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/MED12-Antibody-Immunohistochemistry-Paraffin-NBP1-86669-img0006.jpg)
![Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/MED12-Antibody-Immunohistochemistry-Paraffin-NBP1-86669-img0007.jpg)
![Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669] - Staining of human spleen.](http://images.novusbio.com/fullsize/MED12-Antibody-Immunohistochemistry-Paraffin-NBP1-86669-img0012.jpg)
![Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/MED12-Antibody-Immunohistochemistry-Paraffin-NBP1-86669-img0013.jpg)
![Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669] - Staining of human testis.](http://images.novusbio.com/fullsize/MED12-Antibody-Immunohistochemistry-Paraffin-NBP1-86669-img0014.jpg)
![Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86670] - Staining of human colon.](http://images.novusbio.com/fullsize/MED12-Antibody-Immunohistochemistry-Paraffin-NBP1-86670-img0008.jpg "Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86670] - Staining of human colon.")
![Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86670] - Staining of human rectum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/MED12-Antibody-Immunohistochemistry-Paraffin-NBP1-86670-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86670] - Staining of human cerebral cortex, colon, spleen and testis using Anti-MED12 antibody NBP1-86670 (A) shows similar protein distribution across tissues to independent antibody NBP1-86669 (B).](http://images.novusbio.com/fullsize/MED12-Antibody-Immunohistochemistry-Paraffin-NBP1-86670-img0005.jpg)
![Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86670] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/MED12-Antibody-Immunohistochemistry-Paraffin-NBP1-86670-img0006.jpg)
![Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86670] - Staining of human testis.](http://images.novusbio.com/fullsize/MED12-Antibody-Immunohistochemistry-Paraffin-NBP1-86670-img0007.jpg)
![Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86670] - Staining of human spleen.](http://images.novusbio.com/fullsize/MED12-Antibody-Immunohistochemistry-Paraffin-NBP1-86670-img0009.jpg)
![Western Blot: MED12 Antibody [NBP3-32585] - Western blot analysis of MED12 on different lysates with Rabbit anti-MED12 antibody (NBP3-32585) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: K562 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 243 kDa Observed band size: 243 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32585) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32585_rabbit-med12-pab-278202416285247.jpg "Western Blot: MED12 Antibody [NBP3-32585] - Western blot analysis of MED12 on different lysates with Rabbit anti-MED12 antibody (NBP3-32585) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: K562 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 243 kDa Observed band size: 243 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32585) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MED12 Antibody [NBP3-32585] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-MED12 antibody (NBP3-32585) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32585) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32585_rabbit-med12-pab-29820241492690.jpg)
