| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-2024AF405 | |||
| Alexa Fluor 488 | NB21-2024AF488 | |||
| Alexa Fluor 647 | NB21-2024AF647 | |||
| Alexa Fluor 700 | NB21-2024AF700 | |||
| Biotin | NB21-2024B | |||
| DyLight 405 | NB21-2024V | |||
| DyLight 488 | NB21-2024G | |||
| DyLight 550 | NB21-2024R | |||
| DyLight 650 | NB21-2024C | |||
| FITC | NB21-2024F | |||
| HRP | NB21-2024H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-2024SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-2077AF405 | |||
| Alexa Fluor 488 | NB21-2077AF488 | |||
| Alexa Fluor 647 | NB21-2077AF647 | |||
| Alexa Fluor 700 | NB21-2077AF700 | |||
| Biotin | NB21-2077B | |||
| DyLight 405 | NB21-2077V | |||
| DyLight 488 | NB21-2077G | |||
| DyLight 550 | NB21-2077R | |||
| DyLight 650 | NB21-2077C | |||
| FITC | NB21-2077F | |||
| HRP | NB21-2077H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-2077SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 405 | NB21-2044V | |||
| FITC | NB21-2044F | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-2044SS | |||
![ELISA: HIST1H2AC Antibody (4F10) [H00008334-M01] - Detection limit for recombinant GST tagged HIST1H2AC is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HIST1H2AC-Antibody-4F10-ELISA-H00008334-M01-img0003.jpg "ELISA: HIST1H2AC Antibody (4F10) [H00008334-M01] - Detection limit for recombinant GST tagged HIST1H2AC is 0.1 ng/ml as a capture antibody.")
![Western Blot: HIST1H2AC Overexpression Lysate (Adult Normal) [NBL1-11557] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H2AC.](http://images.novusbio.com/fullsize/HIST1H2AC-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11557-img0002.jpg "Western Blot: HIST1H2AC Overexpression Lysate (Adult Normal) [NBL1-11557] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H2AC.")
![Western Blot: Histone H4 [ac Lys5] Antibody [NB21-2024] - Analysis of H4K5ac in NIH 3t3 histone prep.](http://images.novusbio.com/fullsize/Histone-H4-[ac--Lys5]-Antibody-Western-Blot-NB21-2024-img0010.jpg "Western Blot: Histone H4 [ac Lys5] Antibody [NB21-2024] - Analysis of H4K5ac in NIH 3t3 histone prep.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys5] Antibody [NB21-2024] - Histone H4 K5ac antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H4-[ac--Lys5]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2024-img0007.jpg)
![Chromatin Immunoprecipitation: Histone H4 [ac Lys5] Antibody [NB21-2024] - 2 ug of NB21-2024 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/Histone-H4-[ac--Lys5]-Antibody-Chromatin-Immunoprecipitation-NB21-2024-img0008.jpg)
![Western Blot: Histone H4 [ac Lys5] Antibody [NB21-2024] - Analysis of H4K5ac in HeLa histone prep.](http://images.novusbio.com/fullsize/Histone-H4-[ac--Lys5]-Antibody-Western-Blot-NB21-2024-img0009.jpg)
![Histone H4 [ac Lys5] Antibody [NB21-2024]](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5]-Antibody-NB21-2024-img0011.jpg)
![Western Blot: Histone H4 [ac Lys16] Antibody [NB21-2077] - Western Blot using NB21-2077 of porcine whole ovarian homogenate. Image submitted via verified customer review.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys16]-Antibody-Western-Blot-NB21-2077-img0008.jpg "Western Blot: Histone H4 [ac Lys16] Antibody [NB21-2077] - Western Blot using NB21-2077 of porcine whole ovarian homogenate. Image submitted via verified customer review.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys16] Antibody [NB21-2077] - Histone H4 K16ac antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Histone-H4-[ac--Lys16]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2077-img0005.jpg)
![Western Blot: Histone H4 [ac Lys16] Antibody [NB21-2077] - WB analysis of H4K16ac in 1) HeLa histone prep lysate and 2) NIH 3T3 histone prep lysate.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys16]-Antibody-Western-Blot-NB21-2077-img0002.jpg)
![Dot Blot: Histone H4 [ac Lys16] Antibody [NB21-2077] - Dot blot analysis of H4K16ac in picomoles of protein.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys16]-Antibody-Dot-Blot-NB21-2077-img0004.jpg)
![Histone H4 [ac Lys16] Antibody [NB21-2077]](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys16]-Antibody-NB21-2077-img0006.jpg)
![Western Blot: Histone H4 [ac Lys8] Antibody [NB21-2044] - WB analysis of H4 K8ac in 1) HeLa histone prep 2) 3T3 histone prep and 3) C. elegans embryo lysate.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Western-Blot-NB21-2044-img0006.jpg "Western Blot: Histone H4 [ac Lys8] Antibody [NB21-2044] - WB analysis of H4 K8ac in 1) HeLa histone prep 2) 3T3 histone prep and 3) C. elegans embryo lysate.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys8] Antibody [NB21-2044] - H4 K8ac antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2044-img0002.jpg)
![Chromatin Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NB21-2044] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of NB21-2044 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Chromatin-Immunoprecipitation-NB21-2044-img0005.jpg)
![Dot Blot: Histone H4 [ac Lys8] Antibody [NB21-2044] - Dot Blot analysis of H4 K8ac using the peptides listed above in 100, 10, and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Dot-Blot-NB21-2044-img0004.jpg)
![Histone H4 [ac Lys8] Antibody [NB21-2044]](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-NB21-2044-img0008.jpg)
![Western Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K9/14ac. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Western-Blot-NBP2-54617-img0003.jpg "Western Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K9/14ac. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - HeLa cells were stained with the antibody against H3K9/14ac and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K9/14ac antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Immunofluorescence-NBP2-54617-img0007.jpg)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - ChIP assays were performed using human HeLa cells, the antibody against H3K9/14ac and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes GAPDH and EIF4A2, used as positive controls, and for the coding region of the inactive MB gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys9-ac-Lys14-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54617-img0001.jpg)
![ELISA: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3K9/14ac. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:4,000.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-ELISA-NBP2-54617-img0002.jpg)
![Dot Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - To test the cross reactivity of the antibody against H3K9/14ac, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K9. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Dot-Blot-NBP2-54617-img0006.jpg)
![Protein Array: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - The specificity of the antibody was further demonstrated by peptide array analyses on an array containing 384 peptides with different combinations of modifications from histone H3, H4, H2A and H2B. The antibody was used at a dilution of 1:2,000. Figure shows the specificity factor, calculated as the ratio of the average intensity of all spots containing the mark, divided by the average intensity of all spots not containing the mark.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Protein-Array-NBP2-54617-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2AE [p Ser1] Antibody (RM216) - Azide and BSA Free [NBP3-26017] - Immunocytochemistry of HeLa cells, using Clone RM216 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-26017_rabbit-histone-h2ae-mab-rm216-azide-and-bsa-free-22620242217442.png "Immunocytochemistry/ Immunofluorescence: Histone H2AE [p Ser1] Antibody (RM216) - Azide and BSA Free [NBP3-26017] - Immunocytochemistry of HeLa cells, using Clone RM216 (red). Actin filaments have been labeled with fluorescein phalloidin (green).")
![Western Blot: Histone H2AE [p Ser1] Antibody (RM216) - Azide and BSA Free [NBP3-26017] - Western Blot of acid extracts of HeLa cells treated (1) or non-treated (2) with Nocodazole, using RM216 at 0.5 ug/mL, showed both Histone H2A and H4 phosphorylated at Serine 1 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-26017_rabbit-histone-h2ae-mab-rm216-azide-and-bsa-free-22620242311071.png)
![Multiplex Immunoassay: Histone H2AE [p Ser1] Antibody (RM216) - Azide and BSA Free [NBP3-26017] - RM216 specifically reacts to both Histone H2A and H4 phosphorylated at Serine 1 (H2AS1p and H4S1p).](http://images.novusbio.com/fullsize/nbp3-26017_rabbit-histone-h2ae-mab-rm216-azide-and-bsa-free-22620242311075.png)
![Western Blot: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of: A. 30 ug HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr) B. 30 ug HeLa whole cell lysate/extract (0.4 uM Trichostatin A treatment for 18 hr) 15 % SDS-PAGE Histone H4 (acetyl Lys8).](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Western-Blot-NBP2-42863-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of: A. 30 ug HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr) B. 30 ug HeLa whole cell lysate/extract (0.4 uM Trichostatin A treatment for 18 hr) 15 % SDS-PAGE Histone H4 (acetyl Lys8).")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (acetyl Lys8) protein stained by Histone H4 (acetyl Lys8) antibody diluted at 1:500. Red: alpha Tubulin antibody [114], a cytoskeleton marker, stained by diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42863-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of rat hind brain. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunohistochemistry-Paraffin-NBP2-42863-img0006.jpg)
![<b>Biological Strategies Validation. </b>Chromatin Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Cross-linked ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 uM for 18 h) and 5 ug of either control rabbit IgG or anti-Histone H4K8ac (acetyl Lys8) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or TEXT12.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Chromatin-Immunoprecipitation-NBP2-42863-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of mouse duodenum. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunohistochemistry-Paraffin-NBP2-42863-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of mouse kidney. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunohistochemistry-Paraffin-NBP2-42863-img0005.jpg)
![Dot Blot: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of anti-Histone H4 (acetyl Lys9) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H4 (acetyl Lys9) antibody 1:5000 dilution. A: Peptide samples of H4K8 un B: Peptide samples of H4K8ac C: Peptide samples of H4K16un D: Peptide samples of H4K16ac.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Dot-Blot-NBP2-42863-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of HeLa whole cell lysate/extract: A. 30 ug whole cell lysate/extract of Histone H4 (acetyl K9) protein expressing HeLa cells (0.4uM TSA treatment for 18 hrs) B. Control with 2.5 ug of pre-immune rabbit IgG C. Immunoprecipitation of Histone H4 (acetyl K9) protein by 2.5 ug of Histone H4 (acetyl K9) antibody . 12% SDS-PAGE The immunoprecipitated Histone H4 (acetyl K9) protein was detected by Histone H4 (acetyl K9). EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunoprecipitation-NBP2-42863-img0007.jpg)
![Western Blot: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Analysis of Un-treated (-) and treated (+, 0.4uM Trichostatin A treatment for 18hr) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 (acetyl Lys16) antibody.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-Western-Blot-NBP2-42858-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Analysis of Un-treated (-) and treated (+, 0.4uM Trichostatin A treatment for 18hr) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 (acetyl Lys16) antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (acetyl Lys16) protein stained by Histone H4 (acetyl Lys16) antibody [1271] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-Immunocytochemistry-Immunofluorescence-NBP2-42858-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Rat kidney. Histone H4K16ac (Acetyl Lys16) stained by Histone H4K16ac (Acetyl Lys16) antibody [1271] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-Immunohistochemistry-Paraffin-NBP2-42858-img0005.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Analysis of mouse brain. Histone H4 (acetyl Lys16) antibody [1271] diluted at 1:500.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-Immunohistochemistry-Paraffin-NBP2-42858-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Analysis of mouse kidney. Histone H4 (acetyl Lys16) antibody [1271] diluted at 1:500.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-Immunohistochemistry-Paraffin-NBP2-42858-img0002.jpg)
![Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Rat brain. Histone H4K16ac (Acetyl Lys16) stained by Histone H4K16ac (Acetyl Lys16) antibody [1271] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-NBP2-42858-img0006.jpg)
![Western Blot: Histone H4 [ac Lys8] Antibody [NBP2-59260] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody directed against H4K8ac diluted 1:200 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Western-Blot-NBP2-59260-img0001.jpg "Western Blot: Histone H4 [ac Lys8] Antibody [NBP2-59260] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody directed against H4K8ac diluted 1:200 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys8] Antibody [NBP2-59260] - Mouse NIH3T3 cells were stained with the antibody against H4K8ac and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H4K8ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunofluorescence-NBP2-59260-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NBP2-59260] - ChIP assays were performed using human HeLa cells, the antibody directed against H4K8ac and optimized PCR primer sets for qPCR. ChIP was performed with a LowCell l# ChIP kit on sheared chromatin from 10,000 cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for the c-fos promoter and for the Sat2 satellite repeat region. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Chromatin-Immunoprecipitation-NBP2-59260-img0003.jpg)
![ELISA: Histone H4 [ac Lys8] Antibody [NBP2-59260] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H4K8ac, crude serum and flow through in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:16,700.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-ELISA-NBP2-59260-img0002.jpg)
![Dot Blot: Histone H4 [ac Lys8] Antibody [NBP2-59260] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H4K8ac with peptides containing other histone modifications and the unmodified H4K8. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Dot-Blot-NBP2-59260-img0006.jpg)
![Western Blot: Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody [NBP2-59252] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4K5,8,12ac . The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5--ac-Lys8--ac-Lys12]-Antibody-Western-Blot-NBP2-59252-img0001.jpg "Western Blot: Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody [NBP2-59252] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4K5,8,12ac . The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody [NBP2-59252] - HeLa cells were stained with Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody and DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. (Top): cells were immunofluorescently labeled with the Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. Subsequent images show staining of the cells with the Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody after incubation of the antibody with 10 ng/ul of the following blocking peptides: H4K5,8,12 unmodified, H4K5,8,12ac, H2A.ZK5,7,11ac and H4K5,8,12,16ac, respectively.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5--ac-Lys8--ac-Lys12]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59252-img0005.jpg)
![Chromatin Immunoprecipitation: Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody [NBP2-59252] - ChIP assays were performed using human K562 cells, the antibody against H4K5,8,12ac and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP- seq kit on sheared chromatin from 100,000 cells. A titration of the antibody consisting of 0.2, 0.5, 1 and 2 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for promoter of the active gene EIF4A2 and for a region 1 kb upstream of the GAPDH gene, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5-ac-Lys8-ac-Lys12]-Antibody-Chromatin-Immunoprecipitation-NBP2-59252-img0003.jpg)
![ELISA: Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody [NBP2-59252] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H4K5,8,12ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:14,500.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5--ac-Lys8--ac-Lys12]-Antibody-ELISA-NBP2-59252-img0002.jpg)
![Dot Blot: Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody [NBP2-59252] - To test the cross reactivity of Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5--ac-Lys8--ac-Lys12]-Antibody-Dot-Blot-NBP2-59252-img0004.jpg)