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![Immunohistochemistry-Paraffin: Glutaminase Antibody [NBP1-89766] - Staining in human kidney and skeletal muscle tissues using NBP1-89766 antibody. Corresponding GLS RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Glutaminase-Antibody-Immunohistochemistry-Paraffin-NBP1-89766-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Glutaminase Antibody [NBP1-89766] - Staining in human kidney and skeletal muscle tissues using NBP1-89766 antibody. Corresponding GLS RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Glutaminase Antibody [NBP1-89766] - Region-based expression of the pyruvate recycling pathway marker protein glutaminase (GLT) the VMN of icv Lz-pretreated male and female rats. GLT protein was measured by Western blot in the caudal (c; F(7,40) = 11.67; p < 0.0001) VMN of V/V, V/INS, Lz/V, and Lz/INS groups of male (M; left-hand side) and female (F; right-hand side) rats. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each VMN segment, data depict mean normalized GAD O.D. values +/- S.E.M. VMN = Ventromedial hypothalamic nucleus; V = Vehicle; L = letrozole; INS = insulin. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33238883/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glutaminase-Antibody-Western-Blot-NBP1-89766-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Glutaminase Antibody [NBP1-89766] - Staining of human cell line SiHa shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Glutaminase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89766-img0007.jpg)
![Western Blot: Glutaminase Antibody [NBP1-89766] - Analysis in human cell line EFO-21.](http://images.novusbio.com/fullsize/Glutaminase-Antibody-Western-Blot-NBP1-89766-img0006.jpg)
![Immunohistochemistry-Paraffin: Glutaminase Antibody [NBP1-89766] - Staining of human cerebral cortex shows moderate to strong granular cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Glutaminase-Antibody-Immunohistochemistry-Paraffin-NBP1-89766-img0011.jpg)
![Immunohistochemistry-Paraffin: Glutaminase Antibody [NBP1-89766] - Staining of human duodenum shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Glutaminase-Antibody-Immunohistochemistry-Paraffin-NBP1-89766-img0012.jpg)
![Immunohistochemistry-Paraffin: Glutaminase Antibody [NBP1-89766] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Glutaminase-Antibody-Immunohistochemistry-Paraffin-NBP1-89766-img0013.jpg)
![Immunohistochemistry-Paraffin: Glutaminase Antibody [NBP1-89766] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Glutaminase-Antibody-Immunohistochemistry-Paraffin-NBP1-89766-img0014.jpg)
![Western Blot: Glutaminase Antibody [NBP1-89766] - Region-based patterns of glycogen phosphorylase-muscle type (GPmm) Protein expression in icv Lz-pretreated male & female rats. GPmm protein was measured by Western blot in the rostral (a; F(7,40) = 35.87; p < 0.0001), middle (b; F(7,40) = 33.89; p < 0.0001), & caudal (c; F(7,40) = 9.43; p < 0.0001) VMN of V/V, V/INS, Lz/V, & Lz/INS groups of male (M; left-hand side) & female (F; right-hand side) rats. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each VMN segment, data depict mean normalized GAD O.D. values ± S.E.M. Results are summarized in the table at bottom; ↑ & ↓ denote a relative increase or decrease, respectively, between treatment groups; no change (NC) between groups is also indicated Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33238883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89766_rabbit-polyclonal-glutaminase-antibody-310202415175258.jpg)
![Western Blot: Glutaminase Antibody [NBP1-89766] - Region-based expression of the pyruvate recycling pathway marker protein glutaminase (GLT) the VMN of icv Lz-pretreated male & female rats. GLT protein was measured by Western blot in the rostral (a; F(7,40) = 12.20; p < 0.0001), middle (b; F(7,40) = 17.39; p < 0.0001), & caudal (c; F(7,40) = 11.67; p < 0.0001) VMN of V/V, V/INS, Lz/V, & Lz/INS groups of male (M; left-hand side) & female (F; right-hand side) rats. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each VMN segment, data depict mean normalized GAD O.D. values ± S.E.M. Results are summarized in the table at bottom; ↑ & ↓ denote a relative increase or decrease, respectively, between treatment groups; no change (NC) between groups is also indicated Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33238883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89766_rabbit-polyclonal-glutaminase-antibody-310202415371919.jpg)
![Western Blot: Glutaminase Antibody [NBP1-89766] - Region-based expression of the pyruvate recycling pathway marker protein glutaminase (GLT) the VMN of icv Lz-pretreated male & female rats. GLT protein was measured by Western blot in the rostral (a; F(7,40) = 12.20; p < 0.0001), middle (b; F(7,40) = 17.39; p < 0.0001), & caudal (c; F(7,40) = 11.67; p < 0.0001) VMN of V/V, V/INS, Lz/V, & Lz/INS groups of male (M; left-hand side) & female (F; right-hand side) rats. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each VMN segment, data depict mean normalized GAD O.D. values ± S.E.M. Results are summarized in the table at bottom; ↑ & ↓ denote a relative increase or decrease, respectively, between treatment groups; no change (NC) between groups is also indicated Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33238883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89766_rabbit-polyclonal-glutaminase-antibody-310202415171322.jpg)
![Western Blot: Glutaminase Antibody [NBP1-89766] - Region-based expression of the pyruvate recycling pathway marker protein glutaminase (GLT) the VMN of icv Lz-pretreated male & female rats. GLT protein was measured by Western blot in the rostral (a; F(7,40) = 12.20; p < 0.0001), middle (b; F(7,40) = 17.39; p < 0.0001), & caudal (c; F(7,40) = 11.67; p < 0.0001) VMN of V/V, V/INS, Lz/V, & Lz/INS groups of male (M; left-hand side) & female (F; right-hand side) rats. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each VMN segment, data depict mean normalized GAD O.D. values ± S.E.M. Results are summarized in the table at bottom; ↑ & ↓ denote a relative increase or decrease, respectively, between treatment groups; no change (NC) between groups is also indicated Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33238883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89766_rabbit-polyclonal-glutaminase-antibody-31020241536372.jpg)
![Western Blot: Glutaminase Antibody (5C4) [H00002744-M01] - Western Blot analysis of GLS expression in Hela NE ( Cat # L013V3 ).](http://images.novusbio.com/fullsize/Glutaminase-Antibody-5C4-Western-Blot-H00002744-M01-img0001.jpg "Western Blot: Glutaminase Antibody (5C4) [H00002744-M01] - Western Blot analysis of GLS expression in Hela NE ( Cat # L013V3 ).")
![Immunocytochemistry/Immunofluorescence: Glutaminase Antibody (5C4) [H00002744-M01] - Analysis of monoclonal antibody to GLS on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Glutaminase-Antibody-5C4-Immunocytochemistry-Immunofluorescence-H00002744-M01-img0004.jpg)
![ELISA: Glutaminase Antibody (5C4) [H00002744-M01] - Detection limit for recombinant GST tagged GLS is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Glutaminase-Antibody-5C4-ELISA-H00002744-M01-img0006.jpg)
![Western Blot: Glutaminase Antibody (3211) [NBP2-43762] - Analysis of various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase C (GAC) antibody [3211] diluted at a dilution of 1:1000.](http://images.novusbio.com/fullsize/Glutaminase-Antibody-3211-Western-Blot-NBP2-43762-img0001.jpg "Western Blot: Glutaminase Antibody (3211) [NBP2-43762] - Analysis of various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase C (GAC) antibody [3211] diluted at a dilution of 1:1000.")
![Immunocytochemistry/Immunofluorescence: Glutaminase Antibody (3211) [NBP2-43762] - A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glutaminase C protein stained by Glutaminase C antibody [3211] diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Glutaminase-Antibody-3211-Immunocytochemistry-Immunofluorescence-NBP2-43762-img0002.jpg)
![Immunohistochemistry-Paraffin: Glutaminase Antibody (3211) [NBP2-43762] - Mouse liver. Glutaminase C antibody [3211] diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Glutaminase-Antibody-3211-Immunohistochemistry-Paraffin-NBP2-43762-img0003.jpg)
![Western Blot: Glutaminase Antibody (GT1075) [NBP3-13512] - Glutaminase (GAC) antibody detects Glutaminase (GAC) protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase (GAC) antibody (NBP3-13512) diluted at a dilution of 1:1000.](http://images.novusbio.com/fullsize/Glutaminase-Antibody-GT1075-Western-Blot-NBP3-13512-img0001.jpg "Western Blot: Glutaminase Antibody (GT1075) [NBP3-13512] - Glutaminase (GAC) antibody detects Glutaminase (GAC) protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase (GAC) antibody (NBP3-13512) diluted at a dilution of 1:1000.")
![Immunocytochemistry/Immunofluorescence: Glutaminase Antibody (GT1075) [NBP3-13512] - Glutaminase antibody [GT1075] detects Glutaminase protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glutaminase protein stained by Glutaminase antibody [GT1075] (NBP3-13512) diluted at 1:400. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Glutaminase-Antibody-GT1075-Immunocytochemistry-Immunofluorescence-NBP3-13512-img0002.jpg)
![Western Blot: Glutaminase Antibody (8R8J6) [NBP3-15340] - Western blot analysis of extracts of various cell lines, using Glutaminase (GLS) Rabbit mAb (NBP3-15340) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Glutaminase-Antibody-8R8J6-Western-Blot-NBP3-15340-img0002.jpg "Western Blot: Glutaminase Antibody (8R8J6) [NBP3-15340] - Western blot analysis of extracts of various cell lines, using Glutaminase (GLS) Rabbit mAb (NBP3-15340) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunocytochemistry/Immunofluorescence: Glutaminase Antibody (8R8J6) [NBP3-15340] - Immunofluorescence analysis of U-2 OS cells using Glutaminase (GLS) Rabbit mAb (NBP3-15340) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Glutaminase-Antibody-8R8J6-Immunocytochemistry-Immunofluorescence-NBP3-15340-img0001.jpg)
![Western Blot: Glutaminase Antibody [NBP1-58044] - WB Suggested Anti-GLS Antibody Titration: 0.2-1 ug/ml Positive Control: ACHN cell lysate](http://images.novusbio.com/fullsize/Glutaminase-Antibody-Western-Blot-NBP1-58044-img0002.jpg "Western Blot: Glutaminase Antibody [NBP1-58044] - WB Suggested Anti-GLS Antibody Titration: 0.2-1 ug/ml Positive Control: ACHN cell lysate")
![Western Blot: Glutaminase Antibody (S08-3D8) [NBP3-14937] - Western blot detection of Glutaminase in K562, 3T3, Hela cell lysates using Glutaminase Rabbit mAb (1:1000 diluted). Predicted band size: 74kDa. Observed band size: 68kDa.](http://images.novusbio.com/fullsize/Glutaminase-Antibody-S08-3D8-Western-Blot-NBP3-14937-img0001.jpg "Western Blot: Glutaminase Antibody (S08-3D8) [NBP3-14937] - Western blot detection of Glutaminase in K562, 3T3, Hela cell lysates using Glutaminase Rabbit mAb (1:1000 diluted). Predicted band size: 74kDa. Observed band size: 68kDa.")
![Glutaminase Activity Assay Kit (Colorimetric) [NBP3-24496] - Standard Reference Curve](http://images.novusbio.com/fullsize/nbp3-24496_glutaminase-activity-assay-kit-colorimetric-2611202315561414.png "Glutaminase Activity Assay Kit (Colorimetric) [NBP3-24496] - Standard Reference Curve")
![Glutaminase Activity Assay Kit (Colorimetric) [NBP3-24496] - Assay Example](http://images.novusbio.com/fullsize/nbp3-24496_glutaminase-activity-assay-kit-colorimetric-812024112164.png)
![Western Blot: Glutaminase Overexpression Lysate (Adult Normal) [NBP2-10302] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Glutaminase.](http://images.novusbio.com/fullsize/Glutaminase-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10302-img0001.jpg "Western Blot: Glutaminase Overexpression Lysate (Adult Normal) [NBP2-10302] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Glutaminase.")
![Sandwich ELISA: Glutaminase Antibody (6E12) [H00002744-M05] - Detection limit for recombinant GST tagged GLS is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Glutaminase-Antibody-6E12-Sandwich-ELISA-H00002744-M05-img0001.jpg "Sandwich ELISA: Glutaminase Antibody (6E12) [H00002744-M05] - Detection limit for recombinant GST tagged GLS is 0.03 ng/ml as a capture antibody.")
![Sandwich ELISA: Glutaminase Antibody (6H1) [H00002744-M02] - Detection limit for recombinant GST tagged GLS is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Glutaminase-Antibody-6H1-Sandwich-ELISA-H00002744-M02-img0001.jpg "Sandwich ELISA: Glutaminase Antibody (6H1) [H00002744-M02] - Detection limit for recombinant GST tagged GLS is 0.03 ng/ml as a capture antibody.")
![Sandwich ELISA: Glutaminase Antibody (5B7) [H00002744-M04] - Detection limit for recombinant GST tagged GLS is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Glutaminase-Antibody-5B7-Sandwich-ELISA-H00002744-M04-img0001.jpg "Sandwich ELISA: Glutaminase Antibody (5B7) [H00002744-M04] - Detection limit for recombinant GST tagged GLS is 0.03 ng/ml as a capture antibody.")