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![Knockdown Validated: Fumarase Antibody [NBP1-31336] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NBP1-31336-img0013.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: Fumarase Antibody [NBP1-31336] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: Fumarase Antibody [NBP1-31336] - Representative western blots showing protein expression of fumarase and key enzymes in OXPHOS. VDAC was used as a loading control (n = 3). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep22788) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NBP1-31336-img0018.jpg)
![Immunohistochemistry-Paraffin: Fumarase Antibody [NBP1-31336] - Analysis of paraffin-embedded NCIN87 xenograft, using fumarate hydratase antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Immunohistochemistry-Paraffin-NBP1-31336-img0009.jpg)
![Western Blot: Fumarase Antibody [NBP1-31336] - A. 50 ug mouse liver lysate/extract 7.5% SDS-PAGE Fumarate hydratase antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NBP1-31336-img0010.jpg)
![Western Blot: Fumarase Antibody [NBP1-31336] - A.50 ug rat liver lysate/extract 10% SDS-PAGE Fumarate hydratase antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NBP1-31336-img0011.jpg)
![Western Blot: Fumarase Antibody [NBP1-31336] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarase antibody (NBP1-31336) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31336_rabbit-polyclonal-fumarase-antibody-91020242075950.jpg)
![Immunocytochemistry/ Immunofluorescence: Fumarase Antibody [NBP1-31336] - Fumarase antibody detects Fumarase protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Fumarase stained by Fumarase antibody (NBP1-31336) diluted at 1:500.Blue: Fluoroshield with DAPI .Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-31336_rabbit-polyclonal-fumarase-antibody-910202420122069.jpg)
![Western Blot: Fumarase Antibody (1F10) [NBP1-47754] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Fumarase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Western-Blot-NBP1-47754-img0007.jpg "Western Blot: Fumarase Antibody (1F10) [NBP1-47754] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Fumarase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: Fumarase Antibody (1F10) [NBP1-47754] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Fumarase.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Immunocytochemistry-Immunofluorescence-NBP1-47754-img0006.jpg)
![Immunohistochemistry: Fumarase Antibody (OTI1F10) [NBP1-47754] - Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human colon tissue using anti-Fumarase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-OTI1F10-Immunohistochemistry-NBP1-47754-img0008.jpg)
![Flow Cytometry: Fumarase Antibody (1F10) [NBP1-47754] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Fumarase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Flow-Cytometry-NBP1-47754-img0001.jpg)
![Western Blot: Fumarase Antibody (1F10) [NBP1-47754] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Fumarase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Fumarase.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Western-Blot-NBP1-47754-img0002.jpg)
![Immunohistochemistry-Paraffin: Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human endometrium tissue using anti-Fumarase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Immunohistochemistry-Paraffin-NBP1-47754-img0004.jpg)
![Immunohistochemistry-Paraffin: Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human Kidney tissue using anti-Fumarase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Immunohistochemistry-Paraffin-NBP1-47754-img0005.jpg)
![Western Blot: Fumarase Antibody [NBP1-89814] - Analysis using Anti-FH antibody NBP1-89814 (A) shows similar pattern to independent antibody NBP1-89813 (B).](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NBP1-89814-img0018.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Fumarase Antibody [NBP1-89814] - Analysis using Anti-FH antibody NBP1-89814 (A) shows similar pattern to independent antibody NBP1-89813 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Fumarase Antibody [NBP1-89814] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89814-img0015.jpg)
![Western Blot: Fumarase Antibody [NBP1-89814] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NBP1-89814-img0017.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of mouse hippocampus shows neuronal immunoreactivity in the CA3 layer.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-175202313342022.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of mouse midbrain shows neuronal cell bodies in the substantia nigra pars reticulata.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-1752023133403.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-175202313342060.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of mouse globus pallidus shows immunoreactivity in neurons.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-1752023133407.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-17520231334201.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of mouse medulla shows positivity in the neurons of the facial nucleus.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-175202313342036.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of human testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-1752023133402.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-175202313342070.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of mouse olfactory bulb shows neuronal positivity in the anterior olfactory nucleus.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-17520231334012.jpg)
![Western Blot: Fumarase Antibody [NBP1-32893] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody [N2C2], Internal. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NBP1-32893-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Fumarase Antibody [NBP1-32893] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody [N2C2], Internal. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Fumarase Antibody [NBP1-32893] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32893-img0007.jpg)
![Immunohistochemistry-Paraffin: Fumarase Antibody [NBP1-32893] - Paraffin-embedded OVCAR3 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Immunohistochemistry-Paraffin-NBP1-32893-img0003.jpg)
![Western Blot: Fumarase Antibody [NBP1-32893] - Mouse liver lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NBP1-32893-img0008.jpg)
![Western Blot: Fumarase Antibody [NBP1-32893] - Rat liver lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:5000.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NBP1-32893-img0009.jpg)
![Western Blot: Fumarase Antibody [NBP1-32893] - Sample (30 ug of whole cell lysate) A: 293T B: A431 10% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NBP1-32893-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: Fumarase Antibody [NBP1-32893] - Fumarase antibody [N2C2], Internal detects Fumarase protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Fumarase protein stained by Fumarase antibody [N2C2], Internal (NBP1-32893) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp1-32893_rabbit-polyclonal-fumarase-antibody-91020242091444.jpg)
![Western Blot: Fumarase Antibody [NBP1-89813] - Analysis using Anti-FH antibody NBP1-89813 (A) shows similar pattern to independent antibody NBP1-89814 (B).](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NBP1-89813-img0013.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Fumarase Antibody [NBP1-89813] - Analysis using Anti-FH antibody NBP1-89813 (A) shows similar pattern to independent antibody NBP1-89814 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Fumarase Antibody [NBP1-89813] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Immunohistochemistry-Paraffin-NBP1-89813-img0012.jpg)
![Western Blot: Fumarase Antibody [NBP1-89813] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NBP1-89813-img0007.jpg)
![Immunohistochemistry-Paraffin: Fumarase Antibody [NBP1-89813] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Immunohistochemistry-Paraffin-NBP1-89813-img0009.jpg)
![Immunohistochemistry-Paraffin: Fumarase Antibody [NBP1-89813] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Immunohistochemistry-Paraffin-NBP1-89813-img0010.jpg)
![Immunohistochemistry-Paraffin: Fumarase Antibody [NBP1-89813] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Immunohistochemistry-Paraffin-NBP1-89813-img0011.jpg)
![Western Blot: Fumarase Antibody (7612) [NBP2-43578] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody [7612].](http://images.novusbio.com/fullsize/Fumarase-Antibody-7612-Western-Blot-NBP2-43578-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Fumarase Antibody (7612) [NBP2-43578] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody [7612].")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Fumarase Antibody (7612) [NBP2-43578] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Fumarate hydratase protein stained by Fumarate hydratase antibody [7612] diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Fumarase-Antibody-7612-Immunocytochemistry-Immunofluorescence-NBP2-43578-img0006.jpg)
![Western Blot: Fumarase Antibody (7612) [NBP2-43578] - Analysis of A. 30 ug 293T B. 30 ug A431 C. 30 ug HeLa D. 30 ug HepG2 10 % SDS-PAGE Fumarate hydratase antibody [7612] dilution: 1:500.](http://images.novusbio.com/fullsize/Fumarase-Antibody-7612-Western-Blot-NBP2-43578-img0001.jpg)
![Western Blot: Fumarase Antibody (7612) [NBP2-43578] - Analysis of A. 50 ug mouse liver lysate/extract 10 % SDS-PAGE Fumarate hydratase antibody [7612] dilution: 1:1000](http://images.novusbio.com/fullsize/Fumarase-Antibody-7612-Western-Blot-NBP2-43578-img0002.jpg)
![Western Blot: Fumarase Antibody (7612) [NBP2-43578] - Analysis of A. 50 ug rat liver lysate/extract 10 % SDS-PAGE Fumarate hydratase antibody [7612] dilution: 1:1000](http://images.novusbio.com/fullsize/Fumarase-Antibody-7612-Western-Blot-NBP2-43578-img0003.jpg)
![Western Blot: Fumarase Antibody [NBP1-89815] - Analysis in control (vector only transfected HEK293T lysate) and FH over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NBP1-89815-img0005.jpg "Western Blot: Fumarase Antibody [NBP1-89815] - Analysis in control (vector only transfected HEK293T lysate) and FH over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: Fumarase Antibody [NBP1-89815] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Immunohistochemistry-Paraffin-NBP1-89815-img0009.jpg)
![Immunohistochemistry-Paraffin: Fumarase Antibody [NBP1-89815] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Immunohistochemistry-Paraffin-NBP1-89815-img0006.jpg)
![Immunohistochemistry-Paraffin: Fumarase Antibody [NBP1-89815] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Immunohistochemistry-Paraffin-NBP1-89815-img0007.jpg)
![Immunohistochemistry-Paraffin: Fumarase Antibody [NBP1-89815] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Immunohistochemistry-Paraffin-NBP1-89815-img0008.jpg)
![Western Blot: Fumarase Antibody [NB100-61663] - Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NB100-61663-img0001.jpg "Western Blot: Fumarase Antibody [NB100-61663] - Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Fumarase Antibody [NB100-61663] - Staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 3.75 ug/mL.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Immunohistochemistry-Paraffin-NB100-61663-img0003.jpg)
![Immunohistochemistry-Paraffin: Fumarase Antibody [NB100-61663] - Staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 3.75 ug/mL.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Immunohistochemistry-Paraffin-NB100-61663-img0002.jpg)
![Western Blot: Fumarase Antibody (S08-3F2) [NBP3-15029] - Western blot detection of Fumarase in CHO-K1, C6, Jurkat cell lysates using Fumarase Rabbit mAb (1:500 diluted). Predicted band size: 55kDa. Observed band size: 49kDa.](http://images.novusbio.com/fullsize/Fumarase-Antibody-S08-3F2-Western-Blot-NBP3-15029-img0001.jpg "Western Blot: Fumarase Antibody (S08-3F2) [NBP3-15029] - Western blot detection of Fumarase in CHO-K1, C6, Jurkat cell lysates using Fumarase Rabbit mAb (1:500 diluted). Predicted band size: 55kDa. Observed band size: 49kDa.")
![Immunocytochemistry/Immunofluorescence: Fumarase Antibody (S08-3F2) [NBP3-15029] - Immunofluorescence of Fumarase (green) in hela using Fumarase Rabbit mAb at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/Fumarase-Antibody-S08-3F2-Immunocytochemistry-Immunofluorescence-NBP3-15029-img0002.jpg)
![Western Blot: Fumarase Antibody (3F6) [NBP2-59442] - Lane 1: HeLa cell lysates, Lane 2: LnCaP cell lysates, Lane 3: A549 cell lysates, Lane 4: HepG2 cell lysates, Lane 5: 293T cell lysates, Lane 6: K562 cell lysates, Lane 7: Jurkat cell lysates](http://images.novusbio.com/fullsize/Fumarase-Antibody-3F6-Western-Blot-NBP2-59442-img0001.jpg "Western Blot: Fumarase Antibody (3F6) [NBP2-59442] - Lane 1: HeLa cell lysates, Lane 2: LnCaP cell lysates, Lane 3: A549 cell lysates, Lane 4: HepG2 cell lysates, Lane 5: 293T cell lysates, Lane 6: K562 cell lysates, Lane 7: Jurkat cell lysates")
![SDS-Page: Recombinant Human Fumarase Protein [NBC1-18871] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant Human Fumarase Protein-SDS-Page-NBC1-18871-img0002.jpg "SDS-Page: Recombinant Human Fumarase Protein [NBC1-18871] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")