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![Western Blot: CPT1A Antibody [NB100-53791] - Semiquantitative immunoblotting of CPT1A. Protein expression was investigated in the HB of WT EAE (n=6) and Cpt1a P479L EAE mice (n=6). The data were normalized to the density of the beta actin control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31527712/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CPT1A-Antibody-Western-Blot-NB100-53791-img0002.jpg "Western Blot: CPT1A Antibody [NB100-53791] - Semiquantitative immunoblotting of CPT1A. Protein expression was investigated in the HB of WT EAE (n=6) and Cpt1a P479L EAE mice (n=6). The data were normalized to the density of the beta actin control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31527712/) licensed under a CC-BY license.")
![Western Blot: CPT1A Antibody [NB100-53791] - Staining of MCF7 cell lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CPT1A-Antibody-Western-Blot-NB100-53791-img0001.jpg)
![Immunohistochemistry-Paraffin: CPT1A Antibody [NB100-53791] - Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.](http://images.novusbio.com/fullsize/nb100-53791_goat-polyclonal-cpt1a-antibody-16520239183313.jpg)
![Immunohistochemistry-Paraffin: CPT1A Antibody [NB100-53791] - (5ug/ml) staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/nb100-53791_goat-polyclonal-cpt1a-antibody-16520239183326.jpg)
![Knockout Validated: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - Wild-type (WT) and CPT1A knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25423_rabbit-cpt1a-mab-hl1164-azide-and-bsa-free-2120249422686.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - Wild-type (WT) and CPT1A knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - CPT1A antibody [HL1164] detects CPT1A protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded dog intestine. CPT1A stained by CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25423_rabbit-cpt1a-mab-hl1164-azide-and-bsa-free-2120249393596.jpg)
![Western Blot: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25423_rabbit-cpt1a-mab-hl1164-azide-and-bsa-free-21202494084.jpg)
![Immunohistochemistry-Paraffin: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - CPT1A antibody [HL1164] detects CPT1A protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded cat tissues. CPT1A stained by CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25423_rabbit-cpt1a-mab-hl1164-azide-and-bsa-free-2120249383128.jpg)
![Immunohistochemistry-Paraffin: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - CPT1A antibody [HL1164] detects CPT1A protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. CPT1A stained by CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25423_rabbit-cpt1a-mab-hl1164-azide-and-bsa-free-2120249351354.jpg)
![Western Blot: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25423_rabbit-cpt1a-mab-hl1164-azide-and-bsa-free-2120249393541.jpg)
![Immunocytochemistry/ Immunofluorescence: CPT1A Antibody [NBP3-35370] - Immunofluorescence analysis of Hep G2 cells using CPT1A Rabbit pAb at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35370_rabbit-cpt1a-pab-101220241214487.jpg "Immunocytochemistry/ Immunofluorescence: CPT1A Antibody [NBP3-35370] - Immunofluorescence analysis of Hep G2 cells using CPT1A Rabbit pAb at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunohistochemistry: CPT1A Antibody [NBP3-35370] - Immunohistochemistry analysis of paraffin-embedded Human colon using CPT1A Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35370_rabbit-cpt1a-pab-101220241153586.jpg)
![Western Blot: CPT1A Antibody [NBP3-35370] - Western blot analysis of lysates from HeLa cells using CPT1A Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35370_rabbit-cpt1a-pab-1012202411552896.jpg)
![Western Blot: CPT1A Antibody (PSH04-00) [NBP3-32213] - Western blot analysis of CPT1A on different lysates with Rabbit anti-CPT1A antibody (NBP3-32213) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: SK-OV-3 cell lysate Lane 4: MCF7 cell lysate Lane 5: HepG2 cell lysate Lane 6: A549 cell lysate Lane 7: Human kidney tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 88 kDa Observed band size: 88 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32213) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32213_rabbit-cpt1a-mab-psh04-00-278202416285228.jpg "Western Blot: CPT1A Antibody (PSH04-00) [NBP3-32213] - Western blot analysis of CPT1A on different lysates with Rabbit anti-CPT1A antibody (NBP3-32213) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: SK-OV-3 cell lysate Lane 4: MCF7 cell lysate Lane 5: HepG2 cell lysate Lane 6: A549 cell lysate Lane 7: Human kidney tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 88 kDa Observed band size: 88 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32213) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CPT1A Antibody (PSH04-00) [NBP3-32213] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CPT1A antibody (NBP3-32213) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32213) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32213_rabbit-cpt1a-mab-psh04-00-29820241435278.jpg)
![Flow Cytometry: CPT1A Antibody (PSH04-00) [NBP3-32213] - Flow cytometric analysis of HepG2 cells labeling CPT1A. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32213, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32213_rabbit-cpt1a-mab-psh04-00-29202416453278.jpg)
![Western Blot: CPT1A Antibody [NBP1-59608] - Lanes: 1. 45ug capan1 cell lysate 2. 45 ug HPAF cell lysate Primary, Antibody Dilution: 1 : 1000 Secondary Antibody: Anti-Rabbit HRP Secondary, Antibody Dilution: 1 : 5000 Gene name: CPT1A.](http://images.novusbio.com/fullsize/CPT1A-Antibody-Western-Blot-NBP1-59608-img0006.jpg "Western Blot: CPT1A Antibody [NBP1-59608] - Lanes: 1. 45ug capan1 cell lysate 2. 45 ug HPAF cell lysate Primary, Antibody Dilution: 1 : 1000 Secondary Antibody: Anti-Rabbit HRP Secondary, Antibody Dilution: 1 : 5000 Gene name: CPT1A.")
![Western Blot: CPT1A Antibody [NBP1-59608] - 293T cells lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/CPT1A-Antibody-Western-Blot-NBP1-59608-img0002.jpg)
![Western Blot: CPT1A Antibody [NBP1-59608] - Sample Tissue: 293T, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane/lane, Gel Concentration: 0.12](http://images.novusbio.com/fullsize/CPT1A-Antibody-Western-Blot-NBP1-59608-img0005.jpg)
![Western Blot: CPT1A Antibody [NBP2-15993] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [C1C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CPT1A-Antibody-Western-Blot-NBP2-15993-img0004.jpg "Western Blot: CPT1A Antibody [NBP2-15993] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [C1C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: CPT1A Antibody [NBP2-15993] - Mouse kidney. CPT1A stained by CPT1A antibody [C1C2], Internal diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CPT1A-Antibody-Immunohistochemistry-Paraffin-NBP2-15993-img0005.jpg)
![Immunohistochemistry-Paraffin: CPT1A Antibody [NBP2-15993] - MDA-MB157 xenograft, using CPT1A antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CPT1A-Antibody-Immunohistochemistry-Paraffin-NBP2-15993-img0002.jpg)
![Western Blot: CPT1A Antibody (3M6E9) [NBP3-16072] - Analysis of Mouse heart, using CPT1A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/CPT1A-Antibody-3M6E9-Western-Blot-NBP3-16072-img0002.jpg "Western Blot: CPT1A Antibody (3M6E9) [NBP3-16072] - Analysis of Mouse heart, using CPT1A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: CPT1A Antibody (3M6E9) [NBP3-16072] - Analysis of various lysates, using CPT1A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/CPT1A-Antibody-3M6E9-Western-Blot-NBP3-16072-img0001.jpg)
![Western Blot: CPT1A Overexpression Lysate (Adult Normal) [NBP2-09778] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CPT1A.](http://images.novusbio.com/fullsize/CPT1A-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09778-img0001.jpg "Western Blot: CPT1A Overexpression Lysate (Adult Normal) [NBP2-09778] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CPT1A.")
![Western Blot: CPT1A Overexpression Lysate (Adult Normal) [NBL1-09449] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CPT1A.](http://images.novusbio.com/fullsize/CPT1A-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09449-img0002.jpg "Western Blot: CPT1A Overexpression Lysate (Adult Normal) [NBL1-09449] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CPT1A.")
![Western Blot: CPT1A Antibody (8Y1J4) [NBP3-33364] - Western blot analysis of lysates from MCF7 cells using [KD Validated] CPT1A Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-33364_rabbit-cpt1a-mab-8y1j4-91220241410180.jpg "Western Blot: CPT1A Antibody (8Y1J4) [NBP3-33364] - Western blot analysis of lysates from MCF7 cells using [KD Validated] CPT1A Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45s.")
![Sandwich ELISA: CPT1A Antibody (1D3) [H00001374-M02] - Detection limit for recombinant GST tagged CPT1A is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CPT1A-Antibody-1D3-Sandwich-ELISA-H00001374-M02-img0002.jpg "Sandwich ELISA: CPT1A Antibody (1D3) [H00001374-M02] - Detection limit for recombinant GST tagged CPT1A is approximately 1ng/ml as a capture antibody.")