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![Immunocytochemistry/Immunofluorescence: Claudin-5 Antibody (OTI1G4) [NBP2-02259] - Claudin-5 labeled pig SC cells visualized by alexa 594. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Claudin-5-Antibody-1G4-Immunocytochemistry-NBP2-02259-img0003.jpg "Immunocytochemistry/Immunofluorescence: Claudin-5 Antibody (OTI1G4) [NBP2-02259] - Claudin-5 labeled pig SC cells visualized by alexa 594. ICC/IF image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: Claudin-5 Antibody (1G4) [NBP2-02259] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Claudin 5.](http://images.novusbio.com/fullsize/Claudin-5-Antibody-1G4-Immunocytochemistry-Immunofluorescence-NBP2-02259-img0002.jpg)
![Western Blot: Claudin-5 Antibody [NBP2-92846] -analysis of various lysates, using CLDN5 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp2-92846_rabbit-polyclonal-claudin-5-antibody-western-blot-28112023132836..jpg "Western Blot: Claudin-5 Antibody [NBP2-92846] -analysis of various lysates, using CLDN5 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: Claudin-5 Antibody [NBP2-92846] - Analysis of U2OS cells using Claudin-5 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Claudin-5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92846-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Claudin-5 Antibody - Azide and BSA Free [NBP2-92846] - Immunofluorescence analysis of NIH/3T3 cells using Claudin-5 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92846_rabbit-polyclonal-claudin-5-antibody-4220251429069.jpg%3Ft=1706682212)
![ELISA: Human Claudin-5 ELISA Kit (Colorimetric) [NBP2-75332] - Samples were spiked with high concentrations of Human Claudin-5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75332_human-claudin-5-elisa-kit-colorimetric-13220241626156.jpg "ELISA: Human Claudin-5 ELISA Kit (Colorimetric) [NBP2-75332] - Samples were spiked with high concentrations of Human Claudin-5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Claudin-5 ELISA Kit (Colorimetric) [NBP2-75332] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75332_human-claudin-5-elisa-kit-colorimetric-4320248582510.jpg)
![SDS-Page: Recombinant Human Claudin-5 Protein [H00007122-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue](http://images.novusbio.com/fullsize/Recombinant-Human-Claudin-5-Protein-SDS-Page-H00007122-Q01-img0002.jpg "SDS-Page: Recombinant Human Claudin-5 Protein [H00007122-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue")
![ELISA: Rat Claudin-5 ELISA Kit (Colorimetric) [NBP2-75333] - Samples were spiked with high concentrations of Rat Claudin-5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75333_rat-claudin-5-elisa-kit-colorimetric-13220241620441.jpg "ELISA: Rat Claudin-5 ELISA Kit (Colorimetric) [NBP2-75333] - Samples were spiked with high concentrations of Rat Claudin-5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Claudin-5 ELISA Kit (Colorimetric) [NBP2-75333] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75333_rat-claudin-5-elisa-kit-colorimetric-432024932617.jpg)
![Western Blot: Claudin-5 Antibody (SR3444) [NBP3-27772] - Western blot analysis of Claudin-5 expression in human fetal brain lysate.](http://images.novusbio.com/fullsize/nbp3-27772_rabbit-claudin-5-mab-sr3444-75202410133449.jpg "Western Blot: Claudin-5 Antibody (SR3444) [NBP3-27772] - Western blot analysis of Claudin-5 expression in human fetal brain lysate.")
![Western Blot: Claudin 5 Overexpression Lysate (Adult Normal) [NBL1-09247] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Claudin 5.](http://images.novusbio.com/fullsize/Claudin-5-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09247-img0002.jpg "Western Blot: Claudin 5 Overexpression Lysate (Adult Normal) [NBL1-09247] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Claudin 5.")
![Immunohistochemistry: Claudin-5 Antibody (13G6) [NBP3-26652] - Image of Claudin-5 Antibody (13G6) diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.77% DAB.](http://images.novusbio.com/fullsize/nbp3-26652_rabbit-claudin-5-mab-13g6-27220248453211.jpg "Immunohistochemistry: Claudin-5 Antibody (13G6) [NBP3-26652] - Image of Claudin-5 Antibody (13G6) diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.77% DAB.")
![Sandwich ELISA: Claudin-5 Antibody (3D8) [H00007122-M01] - Detection limit for recombinant GST tagged CLDN5 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Claudin-5-Antibody-3D8-Sandwich-ELISA-H00007122-M01-img0002.jpg "Sandwich ELISA: Claudin-5 Antibody (3D8) [H00007122-M01] - Detection limit for recombinant GST tagged CLDN5 is approximately 0.1ng/ml as a capture antibody.")
![Western Blot: JMJD2B Antibody [NB100-74605] - JMJD2B expression increases in hepatic steatotic cell and animal models. HepG2 cells were incubated with a mixture of palmitic acid (PA) and oleic acid (OA) (1:2 ratio) at 800 uM concentrations for 24 h, and intracellular triglyceride (TG) levels were analyzed by a TG assay kit. JMJD2B mRNA and protein levels were examined by qPCR and western blotting, respectively. Data represent means +/- SEM of three independent experiments performed in triplicate. *p < 0.05 vs. no treatment. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S1A. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-31953-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/JMJD2B-Antibody-Western-Blot-NB100-74605-img0009.jpg "<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: JMJD2B Antibody [NB100-74605] - JMJD2B expression increases in hepatic steatotic cell and animal models. HepG2 cells were incubated with a mixture of palmitic acid (PA) and oleic acid (OA) (1:2 ratio) at 800 uM concentrations for 24 h, and intracellular triglyceride (TG) levels were analyzed by a TG assay kit. JMJD2B mRNA and protein levels were examined by qPCR and western blotting, respectively. Data represent means +/- SEM of three independent experiments performed in triplicate. *p < 0.05 vs. no treatment. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S1A. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-31953-x), licensed under a CC-BY license.")
![<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: JMJD2B Antibody [NB100-74605] - Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Dilution used for WB at 0.5 ug/ml.](http://images.novusbio.com/fullsize/JMJD2B-Antibody-Western-Blot-NB100-74605-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: JMJD2B Antibody [NB100-74605] - Detection of Human JMJD2B on HeLa whole cell lysate using NB100-74605. JMJD2B was also immunoprecipitated by rabbit anti-JMJD2B antibody NB100-74604.](http://images.novusbio.com/fullsize/JMJD2B-Antibody-Western-Blot-NB100-74605-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: JMJD2B Antibody [NB100-74605] - Detection of Human JMJD2B by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-JMJD2B antibody NB100-74605 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/JMJD2B-Antibody-Western-Blot-NB100-74605-img0006.jpg)
![Immunoprecipitation: JMJD2B Antibody [NB100-74605] - Detection of human JMJD2B by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-JMJD2B antibody NB100-74605 (lot NB100-74605-2) used for IP at 6 ug/mg lysate. JMJD2B was also immunoprecipitated by a previous lot (lot NB100-74605-1) of this antibody For blotting immunoprecipitated JMJD2B, NB100-74605 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/JMJD2B-Antibody-Immunoprecipitation-NB100-74605-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: JMJD2B Antibody [NB100-74605] - Knockdown of JMJD2B reduces the intracellular TG level and represses PPARgamma2 expression in HepG2 cells. HepG2 cells in 6-well plates were transfected with scramble RNA or JMJD2B siRNA. The levels of JMJD2B, H3K9me, H3K9me2, and H3K9me3 were measured by western blotting. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S3A. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-31953-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/JMJD2B-Antibody-Knockdown-Validated-NB100-74605-img0008.jpg)
![Western Blot: JMJD2B Antibody [NB100-74605] - Gomisin N (GN) represses the expression of JMJD2B, PPAR gamma 2, & PPAR gamma 2 steatosis target genes in HepG2 cells. HepG2 cells were treated with GN (100 μM) for 12 h. (A) JMJD2B expression was assessed by qPCR & western blotting. The full-length western blots corresponding to truncated blots are given in Supplementary Figure S4. (B) PPAR gamma 2 expression was measured by qPCR. (C,D) The expression of PPAR gamma 2 steatosis target genes was assessed by qPCR. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05 vs. no treatment. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30214048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74605_rabbit-polyclonal-jmjd2b-antibody-310202415395913.jpg)
![Western Blot: JMJD2B Antibody [NB100-74605] - Overexpression of JMJD2B increases intracellular TG levels & stimulates PPAR gamma 2 expression in HepG2 cells. HepG2 cells in 6-well plates were infected with Ad-GFP or Ad-JMJD2B. (A) The levels of JMJD2B, H3K9me, H3K9me2, & H3K9me3 were measured by western blotting. The full-length western blots corresponding to truncated blots are provided in Supplementary Figure S2A. (B) Intracellular TG levels were measured by TG assay kit. (C) PPAR gamma 2 expression was assessed by qPCR & western blotting. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S2B. (D,E) The expression of PPAR gamma 2 steatosis target genes was evaluated by qPCR. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05, **p < 0.01 vs. Ad-GFP. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30214048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74605_rabbit-polyclonal-jmjd2b-antibody-310202415525238.jpg)
![Western Blot: JMJD2B Antibody [NB100-74605] - JMJD2B expression increases in hepatic steatotic cell & animal models. (A) HepG2 cells were incubated with a mixture of palmitic acid (PA) & oleic acid (OA) (1:2 ratio) at 800 μM concentrations for 24 h, & intracellular triglyceride (TG) levels were analyzed by a TG assay kit. JMJD2B mRNA & protein levels were examined by qPCR & western blotting, respectively. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05 vs. no treatment. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S1A. (B) HepG2 cells were treated with T0901317 (10 μM) for 24 h, & intracellular triglyceride (TG) levels were measured by a TG assay kit. JMJD2B mRNA & protein levels were examined by qPCR & western blotting, respectively. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05 vs. no treatment. The full-length western blots are presented in Supplementary Figure S1B. (C) Total RNAs were isolated from the livers of HFD-induced obese mice. The JMJD2B mRNA levels were assessed by qPCR. Data represent means ± SEM of 5 mice. *p < 0.05 vs. ND mice. ND: normal diet. HFD: high fat diet. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30214048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74605_rabbit-polyclonal-jmjd2b-antibody-31020241612623.jpg)
![Western Blot: JMJD2B Antibody [NB100-74605] - Knockdown of JMJD2B reduces the intracellular TG level & represses PPAR gamma 2 expression in HepG2 cells. HepG2 cells in 6-well plates were transfected with scramble RNA or JMJD2B siRNA. (A) The levels of JMJD2B, H3K9me, H3K9me2, & H3K9me3 were measured by western blotting. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S3A. (B) Intracellular TG levels were measured by a TG assay kit. (C) PPAR gamma 2 expression was assessed by qPCR & western blotting. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S3B. (D,E) The expression of PPAR gamma 2 steatosis target genes was evaluated by qPCR. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05 vs. scramble RNA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30214048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74605_rabbit-polyclonal-jmjd2b-antibody-310202415525267.jpg)
![Western Blot: JMJD2B Antibody [NB100-74605] - JMJD2B expression increases in hepatic steatotic cell & animal models. (A) HepG2 cells were incubated with a mixture of palmitic acid (PA) & oleic acid (OA) (1:2 ratio) at 800 μM concentrations for 24 h, & intracellular triglyceride (TG) levels were analyzed by a TG assay kit. JMJD2B mRNA & protein levels were examined by qPCR & western blotting, respectively. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05 vs. no treatment. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S1A. (B) HepG2 cells were treated with T0901317 (10 μM) for 24 h, & intracellular triglyceride (TG) levels were measured by a TG assay kit. JMJD2B mRNA & protein levels were examined by qPCR & western blotting, respectively. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05 vs. no treatment. The full-length western blots are presented in Supplementary Figure S1B. (C) Total RNAs were isolated from the livers of HFD-induced obese mice. The JMJD2B mRNA levels were assessed by qPCR. Data represent means ± SEM of 5 mice. *p < 0.05 vs. ND mice. ND: normal diet. HFD: high fat diet. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30214048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74605_rabbit-polyclonal-jmjd2b-antibody-31020241612639.jpg)
![Western Blot: JMJD2B Antibody [NB100-74605] - Overexpression of JMJD2B increases intracellular TG levels & stimulates PPAR gamma 2 expression in HepG2 cells. HepG2 cells in 6-well plates were infected with Ad-GFP or Ad-JMJD2B. (A) The levels of JMJD2B, H3K9me, H3K9me2, & H3K9me3 were measured by western blotting. The full-length western blots corresponding to truncated blots are provided in Supplementary Figure S2A. (B) Intracellular TG levels were measured by TG assay kit. (C) PPAR gamma 2 expression was assessed by qPCR & western blotting. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S2B. (D,E) The expression of PPAR gamma 2 steatosis target genes was evaluated by qPCR. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05, **p < 0.01 vs. Ad-GFP. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30214048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74605_rabbit-polyclonal-jmjd2b-antibody-310202415535110.jpg)
![Immunohistochemistry: JMJD2B Antibody [NB100-74605] - Adenovirus-mediated JMJD2B overexpression stimulated hepatic steatosis in vivo. C57BL/6 mice (8 weeks old) were injected with adenovirus Ad-GFP or Ad-JMJD2B. After injection, Ad-injected mice were fed a HFD for 2 weeks. (A) JMJD2B expression was determined by qPCR (B) Immunostaining of JMJD2B (scale bar = 50 μm). Representative photographs are shown. JMJD2B positive cells were counted in ten random areas at 400× magnification & analyzed by using image J. Quantification of immunostaining assay is represented as percentage of JMJD2B positive cells. (C) Hepatic triglyceride (TG) levels were measured by a TG assay kit. (D) Liver morphology, ORO & H&E staining (scale bar = 100 μm). Representative images are presented. (E) Serum total TG levels. (F) Serum total cholesterol levels. Data represent means ± SEM from 5 mice. *p < 0.05 vs. Ad-GFP-infected mice. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30214048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74605_rabbit-polyclonal-jmjd2b-antibody-31020241555239.jpg)
![Western Blot: SUV39H2 Antibody [NB100-1140] - staining of Rat Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/SUV39H2-Antibody-Western-Blot-NB100-1140-img0003.jpg "Western Blot: SUV39H2 Antibody [NB100-1140] - staining of Rat Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry: SUV39H2 Antibody [NB100-1140] - Dermal Microvascular Endothelial Cells were fixed with 4% Paraformaldehyde followed by immunofluorescence staining using 1:100 dilution of pAb. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/SUV39H2-Antibody-Immunocytochemistry-NB100-1140-img0005.jpg)
![Western Blot: JMJD2B Antibody (8I0I1) [NBP3-16639] - Western blot analysis of extracts of various cell lines, using JMJD2B Rabbit mAb (NBP3-16639) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/JMJD2B-Antibody-8I0I1-Western-Blot-NBP3-16639-img0003.jpg "Western Blot: JMJD2B Antibody (8I0I1) [NBP3-16639] - Western blot analysis of extracts of various cell lines, using JMJD2B Rabbit mAb (NBP3-16639) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: JMJD2B Antibody (8I0I1) [NBP3-16639] - Immunofluorescence analysis of U-2 OS cells using JMJD2B Rabbit mAb (NBP3-16639) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/JMJD2B-Antibody-8I0I1-Immunocytochemistry-Immunofluorescence-NBP3-16639-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: JMJD2B Antibody (8I0I1) [NBP3-16639] - Immunofluorescence analysis of C6 cells using JMJD2B Rabbit mAb (NBP3-16639) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/JMJD2B-Antibody-8I0I1-Immunocytochemistry-Immunofluorescence-NBP3-16639-img0001.jpg)
![Immunoprecipitation: JMJD2B Antibody (8I0I1) [NBP3-16639] - Immunoprecipitation analysis of 300ug extracts of HCT116 cells using 3ug JMJD2B antibody (NBP3-16639). Western blot was performed from the immunoprecipitate using JMJD2B antibody (NBP3-16639) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/JMJD2B-Antibody-8I0I1-Immunoprecipitation-NBP3-16639-img0004.jpg)
![Chromatin Immunoprecipitation- JMJD2B Antibody (8I0I1) [NBP3-16639] - Analysis of extracts of 5637; cells, using KDM4B/JMJD2B antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16639_rabbit-jmjd2b-mab-8i0i1-chromatin-immunoprecipitation-276202384446..jpg)
![Immunocytochemistry/ Immunofluorescence: JMJD2B Antibody (8I0I1) [NBP3-16639] - Immunofluorescence analysis of C6 cells using JMJD2B Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16639_rabbit-jmjd2b-mab-8i0i1-52202511411487.jpg)
![Immunocytochemistry/ Immunofluorescence: JMJD2B Antibody (8I0I1) [NBP3-16639] - Immunofluorescence analysis of U-2 OS cells using JMJD2B Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16639_rabbit-jmjd2b-mab-8i0i1-5220251136516.jpg)
![Chromatin Immunoprecipitation: JMJD2B Antibody (8I0I1) [NBP3-16639] - Chromatin immunoprecipitation analysis of extracts of 5637; cells, using JMJD2B antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16639_rabbit-jmjd2b-mab-8i0i1-52202511374118.jpg)
![Chromatin Immunoprecipitation: JMJD2B Antibody (8I0I1) [JMJD2B] - Chromatin immunoprecipitation analysis of extracts of 5637; cells, using JMJD2B antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16639_rabbit-jmjd2b-mab-8i0i1-52202517311039.jpg)
![Immunocytochemistry/ Immunofluorescence: JMJD2B Antibody (8I0I1) [JMJD2B] - Immunofluorescence analysis of C6 cells using JMJD2B Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16639_rabbit-jmjd2b-mab-8i0i1-52202517333899.jpg)