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Claudin-5 Antibody (OTI1G4) - Azide and BSA Free

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Immunocytochemistry/Immunofluorescence: Claudin-5 Antibody (OTI1G4) - Azide and BSA Free [NBP2-71329] - Claudin-5 labeled pig SC cells visualized by alexa 594. This image was submitted via customer Review.
Immunocytochemistry/ Immunofluorescence: Claudin-5 Antibody (OTI1G4) - Azide and BSA Free [NBP2-71329] - Claudin-5 labeled pig SC cells visualized by alexa 594. This image was submitted via customer Review.
Immunocytochemistry/ Immunofluorescence: Claudin-5 Antibody (OTI1G4) - Azide and BSA Free [NBP2-71329] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Claudin 5.

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications ICC/IF
Clone
OTI1G4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71329AF350
Alexa Fluor 405
NBP2-71329AF405
Alexa Fluor 488
NBP2-71329AF488
Alexa Fluor 532
NBP2-71329AF532
Alexa Fluor 594
NBP2-71329AF594
Alexa Fluor 647
NBP2-71329AF647
Alexa Fluor 700
NBP2-71329AF700
Alexa Fluor 750
NBP2-71329AF750
Allophycocyanin
NBP2-71329APC
Allophycocyanin/Cy7
NBP2-71329APCCY7
Biotin
NBP2-71329B
CoraFluor 1
NBP2-71329CL1
DyLight 350
NBP2-71329UV
DyLight 405
NBP2-71329V
DyLight 488
NBP2-71329G
DyLight 550
NBP2-71329R
DyLight 594
NBP2-71329DL594
DyLight 650
NBP2-71329C
DyLight 680
NBP2-71329FR
DyLight 755
NBP2-71329IR
FITC
NBP2-71329F
HRP
NBP2-71329H
Janelia Fluor 525
NBP2-71329JF525
Janelia Fluor 549
NBP2-71329JF549
Janelia Fluor 646
NBP2-71329JF646
Janelia Fluor 669
NBP2-71329JF669
PE
NBP2-71329PE
PE/Atto594
NBP2-71329PEATT594
PE/Cy5.5
NBP2-71329PECY55
PE/Cy7
NBP2-71329PECY7
PerCP
NBP2-71329PCP
Unconjugated
NBP2-02259
mFluor Violet 450 SE
NBP2-71329MFV450
mFluor Violet 500 SE
NBP2-71329MFV500
mFluor Violet 610 SE
NBP2-71329MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-02259
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Claudin-5 Antibody (OTI1G4) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human CLDN5(NP_003268) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CLDN5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Claudin-5 Antibody (OTI1G4) - Azide and BSA Free

  • AWAL
  • AWALTransmembrane protein deleted in VCFS
  • BEC1
  • claudin 5
  • Claudin5
  • Claudin-5
  • CLDN5
  • CPETRL1
  • TMDVCF
  • TMVCF1
  • TMVCFTMDVCF
  • transmembrane protein deleted in velocardiofacial syndrome

Background

The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specific regions of different tissues and may have an important role in transcellular transport through tight junctions. Claudin-5 is expressed in the endothelial junctions of the rat liver and in junctions of acinar cells of the pancreas. Human claudin-5 is abundantly expressed in adult lung, heart and skeletal muscle and is deleted in patients with velocardiofacial syndrome, which is characterized by cleft palate, facial dysmorphology and conotruncal heart defects.The human claudin-5 gene maps to chromosome 22q11.21.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CLDN5