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![Western Blot: Ataxin 1 Antibody (2F5) [NBP1-51689] - Analysis using ATXN1 mab against HEK293 (1) and ATXN1 higGFC transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Ataxin-1-Antibody-2F5-Western-Blot-NBP1-51689-img0011.jpg "Western Blot: Ataxin 1 Antibody (2F5) [NBP1-51689] - Analysis using ATXN1 mab against HEK293 (1) and ATXN1 higGFC transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Ataxin 1 Antibody (2F5) [NBP1-51689] - Immunofluorescence analysis of NTERA-2 cells using ATXN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Ataxin-1-Antibody-2F5-Immunocytochemistry-Immunofluorescence-NBP1-51689-img0009.jpg)
![Immunohistochemistry-Paraffin: Ataxin 1 Antibody (2F5) [NBP1-51689] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues (left) and lung cancer tissues (right) using ATXN1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Ataxin-1-Antibody-2F5-Immunohistochemistry-Paraffin-NBP1-51689-img0004.jpg)
![Flow Cytometry: Ataxin 1 Antibody (2F5) [NBP1-51689] - Flow cytometric analysis of Jurket cells using ATXN1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Ataxin-1-Antibody-2F5-Flow-Cytometry-NBP1-51689-img0008.jpg)
![Western Blot: Ataxin 1 Antibody (S76-8) [NBP2-42186] - Western Blot analysis of Monkey COS-1 cells transfected with Ataxin- 1 showing detection of ~85 kDa Ataxin 1 protein using Mouse Anti-Ataxin 1 Monoclonal Antibody, Clone S76-8 (NBP2-42186). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS-1 cells transfected with Ataxin- 1. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Ataxin 1 Monoclonal Antibody (NBP2-42186) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~85 kDa.](http://images.novusbio.com/fullsize/Ataxin-1-Antibody-S76-8-Western-Blot-NBP2-42186-img0002.jpg "Western Blot: Ataxin 1 Antibody (S76-8) [NBP2-42186] - Western Blot analysis of Monkey COS-1 cells transfected with Ataxin- 1 showing detection of ~85 kDa Ataxin 1 protein using Mouse Anti-Ataxin 1 Monoclonal Antibody, Clone S76-8 (NBP2-42186). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS-1 cells transfected with Ataxin- 1. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Ataxin 1 Monoclonal Antibody (NBP2-42186) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~85 kDa.")
![Immunocytochemistry/Immunofluorescence: Ataxin 1 Antibody (S76-8) [NBP2-42186] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ataxin 1 Monoclonal Antibody, Clone S76-8 (NBP2-42186). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ataxin 1 Monoclonal Antibody (NBP2-42186) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ataxin 1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Ataxin-1-Antibody-S76-8-Immunocytochemistry-Immunofluorescence-NBP2-42186-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Ataxin 1 Antibody (S76-8) [NBP2-42186] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ataxin 1 Monoclonal Antibody, Clone S76-8 (NBP2-42186). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ataxin 1 Monoclonal Antibody (NBP2-42186) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Ataxin 1 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/Ataxin-1-Antibody-S76-8-Immunocytochemistry-Immunofluorescence-NBP2-42186-img0004.jpg)
![Western Blot: Ataxin 1 Antibody (S65-37) [NBP2-42204] - Western Blot analysis of Monkey COS-1 cells transfected with Ataxin- 1 showing detection of ~85 kDa Ataxin 1 protein using Mouse Anti-Ataxin 1 Monoclonal Antibody, Clone S65-37 (NBP2-42204). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS-1 cells transfected with Ataxin- 1. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Ataxin 1 Monoclonal Antibody (NBP2-42204) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~85 kDa.](http://images.novusbio.com/fullsize/Ataxin-1-Antibody-S65-37-Western-Blot-NBP2-42204-img0003.jpg "Western Blot: Ataxin 1 Antibody (S65-37) [NBP2-42204] - Western Blot analysis of Monkey COS-1 cells transfected with Ataxin- 1 showing detection of ~85 kDa Ataxin 1 protein using Mouse Anti-Ataxin 1 Monoclonal Antibody, Clone S65-37 (NBP2-42204). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS-1 cells transfected with Ataxin- 1. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Ataxin 1 Monoclonal Antibody (NBP2-42204) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~85 kDa.")
![Immunocytochemistry/Immunofluorescence: Ataxin 1 Antibody (S65-37) [NBP2-42204] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ataxin 1 Monoclonal Antibody, Clone S65-37 (NBP2-42204). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ataxin 1 Monoclonal Antibody (NBP2-42204) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ataxin 1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Ataxin-1-Antibody-S65-37-Immunocytochemistry-Immunofluorescence-NBP2-42204-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Ataxin 1 Antibody (S65-37) [NBP2-42204] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ataxin 1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/Ataxin-1-Antibody-S65-37-Immunocytochemistry-Immunofluorescence-NBP2-42204-img0002.jpg)
![Immunohistochemistry-Paraffin: Ataxin 1 Antibody [NBP1-90065] - Staining of human prostate shows weak to moderate nuclear positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Ataxin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-90065-img0009.jpg "Immunohistochemistry-Paraffin: Ataxin 1 Antibody [NBP1-90065] - Staining of human prostate shows weak to moderate nuclear positivity in smooth muscle cells.")
![Immunohistochemistry-Paraffin: Ataxin 1 Antibody [NBP1-90065] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/Ataxin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-90065-img0008.jpg)
![Immunohistochemistry-Paraffin: Ataxin 1 Antibody [NBP1-90065] - Staining of human lymph node shows moderate nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/Ataxin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-90065-img0007.jpg)
![SDS-Page: Recombinant Human Ataxin 1 Protein [H00006310-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Ataxin-1-Protein-SDS-Page-H00006310-Q01-img0002.jpg "SDS-Page: Recombinant Human Ataxin 1 Protein [H00006310-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Ataxin 1 Antibody (4C5) [H00006310-M02] - Detection against Immunogen (36.74 KDa) .](http://images.novusbio.com/fullsize/Ataxin-1-Antibody-4C5-Western-Blot-H00006310-M02-img0001.jpg "Western Blot: Ataxin 1 Antibody (4C5) [H00006310-M02] - Detection against Immunogen (36.74 KDa) .")
![Immunocytochemistry/Immunofluorescence: Ataxin 1 Antibody [NBP2-57735] - Staining of human cell line RT4 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/Ataxin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57735-img0001.jpg "Immunocytochemistry/Immunofluorescence: Ataxin 1 Antibody [NBP2-57735] - Staining of human cell line RT4 shows localization to nucleoplasm & cytosol.")
![ELISA: Mouse Ataxin 1 ELISA Kit (Colorimetric) [NBP2-69895] - Samples were spiked with high concentrations of Mouse Ataxin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69895_mouse-ataxin-1-elisa-kit-colorimetric-13220241620474.jpg "ELISA: Mouse Ataxin 1 ELISA Kit (Colorimetric) [NBP2-69895] - Samples were spiked with high concentrations of Mouse Ataxin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Ataxin 1 ELISA Kit (Colorimetric) [NBP2-69895] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69895_mouse-ataxin-1-elisa-kit-colorimetric-432024932670.jpg)
![ELISA: Human Ataxin 1 ELISA Kit (Colorimetric) [NBP2-69894] - Samples were spiked with high concentrations of Human Ataxin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69894_human-ataxin-1-elisa-kit-colorimetric-132202416225291.jpg "ELISA: Human Ataxin 1 ELISA Kit (Colorimetric) [NBP2-69894] - Samples were spiked with high concentrations of Human Ataxin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Ataxin 1 ELISA Kit (Colorimetric) [NBP2-69894] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69894_human-ataxin-1-elisa-kit-colorimetric-4320249173459.jpg)
![ELISA: Rat Ataxin 1 ELISA Kit (Colorimetric) [NBP2-69896] - Samples were spiked with high concentrations of Rat Ataxin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69896_rat-ataxin-1-elisa-kit-colorimetric-132202416143025.jpg "ELISA: Rat Ataxin 1 ELISA Kit (Colorimetric) [NBP2-69896] - Samples were spiked with high concentrations of Rat Ataxin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Ataxin 1 ELISA Kit (Colorimetric) [NBP2-69896] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69896_rat-ataxin-1-elisa-kit-colorimetric-432024973622.jpg)
![Western Blot: Ataxin 1 Overexpression Lysate (Adult Normal) [NBP2-11144] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Ataxin 1.](http://images.novusbio.com/fullsize/Ataxin-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11144-img0001.jpg "Western Blot: Ataxin 1 Overexpression Lysate (Adult Normal) [NBP2-11144] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Ataxin 1.")