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![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP1-87679] - Analysis in human cerebral cortex and liver tissues. Corresponding AQP4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87679-img0027.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP1-87679] - Analysis in human cerebral cortex and liver tissues. Corresponding AQP4 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Aquaporin-4 Antibody [NBP1-87679] - Analysis in human brain tissue.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Western-Blot-NBP1-87679-img0017.jpg)
![Immunohistochemistry: Aquaporin-4 Antibody [NBP1-87679] - Staining of mouse cerebellum shows positivity in astrocytes, mainly in the molecular layer.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87679-img0029.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP1-87679] - Staining of human cerebellum shows moderate to strong membranous positivity in astrocytes, mainly in the molecular layer.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87679-img0030.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP1-87679] - Staining of human cerebral cortex shows moderate to strong membranous positivity in astrocytes.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87679-img0031.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP1-87679] - Staining of human liver shows no positivity in hepatocytes, as expected.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87679-img0032.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP1-87679] - Staining of human stomach shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87679-img0033.jpg)
![Immunohistochemistry-Frozen: Aquaporin-4 Antibody [NBP1-87679] - Mouse trigeminal ganglion stained with Aquaporin-4 antibody at 1:5000. Image submitted by a verified customer review](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Frozen-NBP1-87679-img0036.jpg)
![Western Blot: Aquaporin-4 Antibody [NBP3-12902] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Aquaporin-4 antibody (NBP3-12902) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Western-Blot-NBP3-12902-img0004.jpg "Western Blot: Aquaporin-4 Antibody [NBP3-12902] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Aquaporin-4 antibody (NBP3-12902) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Aquaporin-4 Antibody [NBP3-12902] - Aquaporin-4 antibody detects Aquaporin-4 protein at astrocyte cells by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons and glia cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Aquaporin-4 protein stained by Aquaporin-4 antibody (NBP3-12902) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (NBP2-43561) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12902-img0002.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP3-12902] - Aquaporin-4 antibody detects Aquaporin-4 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Aquaporin-4 stained by Aquaporin-4 antibody (NBP3-12902) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP3-12902-img0005.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP3-12902] - Aquaporin-4 antibody detects Aquaporin-4 protein at cell membrane in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Aquaporin-4 antibody (NBP3-12902) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP3-12902-img0001.jpg)
![Immunohistochemistry-Frozen: Aquaporin-4 Antibody [NBP3-12902] - Aquaporin-4 antibody detects Aquaporin-4 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: Aquaporin-4 protein stained by Aquaporin-4 antibody (NBP3-12902) diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Frozen-NBP3-12902-img0003.jpg)
![Western Blot: Aquaporin-4 Antibody [NBP2-92886] - Western blot analysis of extracts of SKOV3 cells, using Aquaporin-4 antibody (NBP2-92886) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Aquaporin-4 Antibody-Western Blot-NBP2-92886-img0003.jpg "Western Blot: Aquaporin-4 Antibody [NBP2-92886] - Western blot analysis of extracts of SKOV3 cells, using Aquaporin-4 antibody (NBP2-92886) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP2-92886] - Immunohistochemistry of paraffin-embedded rat brain using Aquaporin-4 Rabbit pAb (NBP2-92886) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP2-92886-img0002.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP2-92886] - Immunohistochemistry of paraffin-embedded mouse kidney using Aquaporin-4 Rabbit pAb (NBP2-92886) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP2-92886-img0001.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining in human cerebral cortex and liver tissues. Corresponding AQP4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-CL0178-Immunohistochemistry-Paraffin-NBP2-52872-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining in human cerebral cortex and liver tissues. Corresponding AQP4 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-CL0178-Western-Blot-NBP2-52872-img0013.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining of human cerebral cortex shows moderate to strong membranous positivity in astrocytes.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-CL0178-Immunohistochemistry-Paraffin-NBP2-52872-img0008.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining of human cerebellum shows strong positivity in neuropil, mainly in the molecular layer.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-CL0178-Immunohistochemistry-Paraffin-NBP2-52872-img0009.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining of human stomach shows moderate to strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-CL0178-Immunohistochemistry-Paraffin-NBP2-52872-img0010.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-CL0178-Immunohistochemistry-Paraffin-NBP2-52872-img0011.jpg)
![Western Blot: Aquaporin-4 Antibody [NBP1-97928] - Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin-4 protein using Rabbit Anti-Aquaporin-4 Polyclonal Antibody (NBP1-97928). Primary Antibody: Rabbit Anti-Aquaporin-4 Polyclonal Antibody (NBP1-97928) at 1:2000.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Western-Blot-NBP1-97928-img0007.jpg "Western Blot: Aquaporin-4 Antibody [NBP1-97928] - Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin-4 protein using Rabbit Anti-Aquaporin-4 Polyclonal Antibody (NBP1-97928). Primary Antibody: Rabbit Anti-Aquaporin-4 Polyclonal Antibody (NBP1-97928) at 1:2000.")
![Immunohistochemistry: Aquaporin-4 Antibody [NBP1-97928] - Immunohistochemistry analysis using Rabbit Anti-Aquaporin-4 Polyclonal Antibody (NBP1-97928). Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin-4 Polyclonal Antibody (NBP1-97928) at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 m cryostat sections.](http://images.novusbio.com/fullsize/Aquaporin-4 Antibody-Immunohistochemistry-NBP1-97928-img0008.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (AQP4/3324) [NBP3-13952] - Formalin-fixed, paraffin-embedded human small intestine stained with Aquaporin-4 antibody (AQP4/3324). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-AQP4-3324-Immunohistochemistry-Paraffin-NBP3-13952-img0003.jpg "Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (AQP4/3324) [NBP3-13952] - Formalin-fixed, paraffin-embedded human small intestine stained with Aquaporin-4 antibody (AQP4/3324). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.")
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (AQP4/3324) [NBP3-13952] - Formalin-fixed, paraffin-embedded human brain stained with Aquaporin-4 antibody (AQP4/3324). Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-AQP4-3324-Immunohistochemistry-Paraffin-NBP3-13952-img0001.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (AQP4/3324) [NBP3-13952] - Formalin-fixed, paraffin-embedded human small intestine stained with Aquaporin-4 antibody (AQP4/3324). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-AQP4-3324-Immunohistochemistry-Paraffin-NBP3-13952-img0002.jpg)
![Protein Array: Aquaporin-4 Antibody (AQP4/3324) [NBP3-13952] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Aquaporin-4 Antibody (AQP4/3324).](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-AQP4-3324-Protein-Array-NBP3-13952-img0005.jpg)
![Western Blot: Aquaporin-4 Antibody (JE59-76) [NBP3-32046] - Western blot analysis of Aquaporin-4 on different lysates with Rabbit anti-Aquaporin-4 antibody (NBP3-32046) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 35 kDa Observed band size: 28/52 kDa Exposure time: Lane 1: 31 seconds; Lane 2-3: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32046) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32046_rabbit-aquaporin-4-mab-je59-76-278202416331236.jpg "Western Blot: Aquaporin-4 Antibody (JE59-76) [NBP3-32046] - Western blot analysis of Aquaporin-4 on different lysates with Rabbit anti-Aquaporin-4 antibody (NBP3-32046) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 35 kDa Observed band size: 28/52 kDa Exposure time: Lane 1: 31 seconds; Lane 2-3: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32046) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Aquaporin-4 Antibody (JE59-76) [NBP3-32046] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Aquaporin-4 antibody (NBP3-32046) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32046) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32046_rabbit-aquaporin-4-mab-je59-76-29820241492659.jpg)
![Western Blot: Aquaporin-4 Antibody [NBP2-87018] - WB Suggested Anti-AQP4 Antibody. Titration: 1.0 ug/ml. Positive Control: MDA-MB-435S Whole CellAQP4 is strongly supported by BioGPS gene expression data to be expressed in Human MDA-MB435 cells](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Western-Blot-NBP2-87018-img0001.jpg "Western Blot: Aquaporin-4 Antibody [NBP2-87018] - WB Suggested Anti-AQP4 Antibody. Titration: 1.0 ug/ml. Positive Control: MDA-MB-435S Whole CellAQP4 is strongly supported by BioGPS gene expression data to be expressed in Human MDA-MB435 cells")
![Western Blot: Aquaporin-4 Antibody [NBP2-87018] - Host: Mouse. Target Name: AQP4. Sample Tissue: Mouse Heart. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Western-Blot-NBP2-87018-img0002.jpg)
![Western Blot: Aquaporin-4 Antibody [NBP2-92730] - Analysis of extracts of various cell lines, using Aquaporin-4 .Exposure time: 90s.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Western-Blot-NBP2-92730-img0004.jpg "Western Blot: Aquaporin-4 Antibody [NBP2-92730] - Analysis of extracts of various cell lines, using Aquaporin-4 .Exposure time: 90s.")
![ELISA: Mouse Aquaporin-4 ELISA Kit (Colorimetric) [NBP2-69852] - Samples were spiked with high concentrations of Mouse Aquaporin-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69852_mouse-aquaporin-4-elisa-kit-colorimetric-132202416254011.jpg "ELISA: Mouse Aquaporin-4 ELISA Kit (Colorimetric) [NBP2-69852] - Samples were spiked with high concentrations of Mouse Aquaporin-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Aquaporin-4 ELISA Kit (Colorimetric) [NBP2-69852] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69852_mouse-aquaporin-4-elisa-kit-colorimetric-432024859750.jpg)
![ELISA: Human Aquaporin-4 ELISA Kit (Colorimetric) [NBP2-69851] - Samples were spiked with high concentrations of Human Aquaporin-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69851_human-aquaporin-4-elisa-kit-colorimetric-13220241620498.jpg "ELISA: Human Aquaporin-4 ELISA Kit (Colorimetric) [NBP2-69851] - Samples were spiked with high concentrations of Human Aquaporin-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Aquaporin-4 ELISA Kit (Colorimetric) [NBP2-69851] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69851_human-aquaporin-4-elisa-kit-colorimetric-4320249173498.jpg)