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YY1 Products By Gene Name

18 Results for "YY1"

NBP2-20932

YY1 Antibody

(1 Publication)

0.1 ml

NBP2-20932

Western Blot: YY1 Antibody [NBP2-20932] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with YY1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: YY1 Antibody [NBP2-20932] - YY1 antibody detects YY1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: YY1 stained by YY1 antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.

Immunohistochemistry-Paraffin: YY1 Antibody [NBP2-20932] - C2C12 xenograft, using YY1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: YY1 Antibody [NBP2-20932] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE gel, diluted at 1:2000.

Western Blot: YY1 Antibody [NBP2-20932] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 10 % SDS-PAGE YY1 antibody dilution: 1:1000

Immunohistochemistry-Paraffin: YY1 Antibody [NBP2-20932] - 59T xenograft, using YY1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP1-46218

YY1 Antibody

(4 Publications)

0.1 ml

NBP1-46218

Western Blot: YY1 Antibody [NBP1-46218] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-YY1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. YY1 was also immunoprecipitated by rabbit anti-YY1 antibody NBP1-46217, which recognizes an upstream epitope.

<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: YY1 Antibody [NBP1-46218] - Section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-YY1 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-85260

YY1 Antibody

(2 Publications)

0.1 ml

NBP1-85260

Western Blot: YY1 Antibody [NBP1-85260] - Analysis in human cell line HL-60.

Immunohistochemistry-Paraffin: YY1 Antibody [NBP1-85260] - Staining of human small intestine shows moderate nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: YY1 Antibody [NBP1-85260] - Staining of human lymph node shows strong nuclear positivity.

Immunohistochemistry-Paraffin: YY1 Antibody [NBP1-85260] - Staining of human pancreas moderate nuclear positivity in exocrine glandular cells.

Immunohistochemistry-Paraffin: YY1 Antibody [NBP1-85260] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP3-15779

YY1 Antibody (6J6L1)

100 ul

NBP3-15779

Western Blot: YY1 Antibody (6J6L1) [NBP3-15779] - Western blot analysis of extracts of various cell lines, using YY1 antibody (NBP3-15779) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunocytochemistry/Immunofluorescence: YY1 Antibody (6J6L1) [NBP3-15779] - Immunofluorescence analysis of C6 cells using YY1 antibody (NBP3-15779). Blue: DAPI for nuclear staining.

Chromatin Immunoprecipitation: YY1 Antibody (6J6L1) [NBP3-15779] - Chromatin immunoprecipitation analysis of extracts of 293T cells, using YY1 antibody (NBP3-15779) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Western Blot: YY1 Antibody (6J6L1) [NBP3-15779] - Western blot analysis of extracts of various cell lines, using YY1 antibody (NBP3-15779) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunoprecipitation: YY1 Antibody (6J6L1) [NBP3-15779] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug YY1 antibody (NBP3-15779). Western blot was performed from the immunoprecipitate using YY1 antibody (NBP3-15779) at a dilition of 1:2000.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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AF3784

YY1 Antibody

100 μg

AF3784

Western blot shows nuclear extracts of Daudi human Burkitt's lymphoma cell line, U937 human histiocytic lymphoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human YY1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3784) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for YY1 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

YY1 was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human YY1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3784) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3784-SP

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H00007528-M01

YY1 Antibody (2C4)

0.1 mg

H00007528-M01

Western Blot: YY1 Antibody (2C4) [H00007528-M01] - YY1 monoclonal antibody (M01), clone 2C4 Analysis of YY1 expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: YY1 Antibody (2C4) [H00007528-M01] - Analysis of monoclonal antibody to YY1 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: YY1 Antibody (2C4) [H00007528-M01] - Analysis of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.

Western Blot: YY1 Antibody (2C4) [H00007528-M01] - Analysis of YY1 expression in transfected 293T cell line by YY1 monoclonal antibody (M01), clone 2C4.Lane 1: YY1 transfected lysate(44.7 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: YY1 Antibody (2C4) [H00007528-M01] - Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00007528-M02

YY1 Antibody (4A5)

(1 Publication)

0.1 mg

H00007528-M02

Western Blot: YY1 Antibody (4A5) [H00007528-M02] - YY1 monoclonal antibody (M02), clone 4A5 Analysis of YY1 expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: YY1 Antibody (4A5) [H00007528-M02] - Analysis of monoclonal antibody to YY1 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: YY1 Antibody (4A5) [H00007528-M02] - Analysis of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.

Sandwich ELISA: YY1 Antibody (4A5) [H00007528-M02] - Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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MAB3784

YY1 Antibody (854847)

(1 Publication)

100 μg

MAB3784

Western blot shows lysates of U937 human histiocytic lymphoma cell line and C2C12 mouse myoblast cell line. Gels were loaded with 30 µg of U937 and 40 µg of C2C12 cytoplasmic (Cyto) extracts and 15 µg of U937 and 20 µg of C2C12 nuclear (Nuc) extracts. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse YY1 Monoclonal Antibody (Catalog # MAB3784) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for YY1 at approximately 60-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB3784-SP

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H00007528-M04

YY1 Antibody (2C5)

0.1 mg

H00007528-M04

Western Blot: YY1 Antibody (2C5) [H00007528-M04] - Analysis of YY1 expression in Hela S3 NE (Cat # L013V3).

Immunocytochemistry/Immunofluorescence: YY1 Antibody (2C5) [H00007528-M04] - Analysis of monoclonal antibody to YY1 on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: YY1 Antibody (2C5) [H00007528-M04] - Analysis of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 7 ug/ml

ELISA: YY1 Antibody (2C5) [H00007528-M04] - Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00007528-M03

YY1 Antibody (4D2)

0.1 mg

H00007528-M03

Western Blot: YY1 Antibody (4D2) [H00007528-M03] - Analysis of YY1 expression in Hela S3 NE (Cat # L013V3).

Immunocytochemistry/Immunofluorescence: YY1 Antibody (4D2) [H00007528-M03] - Analysis of monoclonal antibody to YY1 on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: YY1 Antibody (4D2) [H00007528-M03] - Analysis of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human endometrium. Antibody concentration 3 ug/ml

ELISA: YY1 Antibody (4D2) [H00007528-M03] - Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP2-20933

YY1 Antibody

0.1 ml

NBP2-20933

Western Blot: YY1 Antibody [NBP2-20933] - 30 ug 293T whole cell lysate/extract10 % SDS-PAGE YY1 antibody dilution: 1:1000.

Immunoprecipitation: YY1 Antibody [NBP2-20933] - Sample: 1000 ug HeLa whole cell lysate/extract A. 50 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of YY1protein by 2 ug of YY1 antibody 10% SDS-PAGE The immunoprecipitated YY1 protein was detected by YY1 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.

Reactivity:

Hu, Mu, Rt, Bv

Species Glossary

Reactivity: Hu, Mu, Rt, Bv

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP3-14987

YY1 Antibody (S02-8A3)

100 ul

NBP3-14987

Western Blot: YY1 Antibody (S02-8A3) [NBP3-14987] - Western blot detection of YY1 in K562, Rat Brain, C6, 3T3, Hela cell lysates using YY1 Rabbit mAb (1:1000 diluted). Predicted band size: 45kDa. Observed band size: 65kDa.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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