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![Western Blot: csl/RBPJK Antibody [NBP1-33427] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with csl/RBPJK antibody [C1C3] (NBP1-33427) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody,](http://images.novusbio.com/fullsize/nbp1-33427_rabbit-polyclonal-csl-rbpjk-antibody-91020241959356.jpg "Western Blot: csl/RBPJK Antibody [NBP1-33427] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with csl/RBPJK antibody [C1C3] (NBP1-33427) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody,")
![Immunocytochemistry/Immunofluorescence: csl/RBPJK Antibody [NBP1-33427] - Analysis of paraformaldehyde-fixed HeLa at 1:100 dilution.](http://images.novusbio.com/fullsize/csl-RBPJK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33427-img0004.jpg)
![Chromatin Immunoprecipitation (ChIP): csl/RBPJK Antibody [NBP1-33427] - Sheared, cross-linked chromatin was prepared from Jurkat T-ALL cells using the Covaris TruChIP kit and shearing with the LE220 sonicator. The chromatin buffer was adjusted to 150 mM NaCl, 20 mM Tris-Cl pH 8, 2 mM EDTA, and 1% Triton X-100. Three antibodies, as well as pure pre-immune rabbit IgG control, were added to each IP reaction, consisting of 7 million cells, and incubated overnight with mixing at 4 C. The antibodies were captured with Magna ChIP Protein A+G beads (Millipore), washed with three wash buffers, and the DNA eluted and purified with the iPure v2 kit (Diagenode). The purified DNA was normalized to the input control with qPCR quantification of the insulin exon 1 gene (INS) and an RBPJ binding site in the promoter of HES1. Image from verified customer review.](http://images.novusbio.com/fullsize/csl-RBPJK-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP1-33427-img0006.jpg)
![Western Blot: csl/RBPJK Antibody (9B3L6) [NBP3-16326] - Western blot analysis of extracts of various cell lines, using csl/RBPJK Rabbit mAb (NBP3-16326) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/csl-RBPJK-Antibody-9B3L6-Western-Blot-NBP3-16326-img0003.jpg "Western Blot: csl/RBPJK Antibody (9B3L6) [NBP3-16326] - Western blot analysis of extracts of various cell lines, using csl/RBPJK Rabbit mAb (NBP3-16326) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: csl/RBPJK Antibody (9B3L6) [NBP3-16326] - Immunohistochemistry of paraffin-embedded human lung squamous carcinoma tissue using csl/RBPJK Rabbit mAb (NBP3-16326) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/csl-RBPJK-Antibody-9B3L6-Immunohistochemistry-Paraffin-NBP3-16326-img0002.jpg)
![Immunohistochemistry-Paraffin: csl/RBPJK Antibody (9B3L6) [NBP3-16326] - Immunohistochemistry of paraffin-embedded human colon carcinoma using csl/RBPJK Rabbit mAb (NBP3-16326) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/csl-RBPJK-Antibody-9B3L6-Immunohistochemistry-Paraffin-NBP3-16326-img0001.jpg)
![Western Blot: csl/RBPJK Antibody [H00003516-D01P] - Analysis of RBPJ expression in mouse testis.](http://images.novusbio.com/fullsize/csl-RBPJK-Antibody-Western-Blot-H00003516-D01P-img0005.jpg "Western Blot: csl/RBPJK Antibody [H00003516-D01P] - Analysis of RBPJ expression in mouse testis.")
![Western Blot: csl/RBPJK Antibody [H00003516-D01P] - Analysis of RBPJ expression in human placenta.](http://images.novusbio.com/fullsize/csl-RBPJK-Antibody-Western-Blot-H00003516-D01P-img0004.jpg)
![Western Blot: csl/RBPJK Antibody [H00003516-D01P] - Analysis of RBPJ expression in transfected 293T cell line by RBPJ polyclonal antibody.Lane 1: RBPJ transfected lysate(54.30 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/csl-RBPJK-Antibody-Western-Blot-H00003516-D01P-img0006.jpg)
![Immunohistochemistry: csl/RBPJK Antibody [NBP3-10894] - Immunohistochemical analysis of human liver.](http://images.novusbio.com/fullsize/csl-RBPJK-Antibody-Immunohistochemistry-NBP3-10894-img0001.jpg "Immunohistochemistry: csl/RBPJK Antibody [NBP3-10894] - Immunohistochemical analysis of human liver.")
![Western Blot: csl/RBPJK Antibody (S05-6G4) [NBP3-19759] - Western blot detection of csl/RBPJK in Rat Brain, C6 cell lysates using NBP3-19759 (1:1000 diluted). Predicted band size: 56kDa. Observed band size: 61kDa.](http://images.novusbio.com/fullsize/csl-RBPJK-Antibody-S05-6G4-Western-Blot-NBP3-19759-img0001.jpg "Western Blot: csl/RBPJK Antibody (S05-6G4) [NBP3-19759] - Western blot detection of csl/RBPJK in Rat Brain, C6 cell lysates using NBP3-19759 (1:1000 diluted). Predicted band size: 56kDa. Observed band size: 61kDa.")
![Western Blot: csl/RBPJK Antibody (4E12) [H00003516-M01] - Analysis of RBPJ expression in transfected 293T cell line by RBPJ monoclonal antibody (M01), clone 4E12.Lane 1: RBPJ transfected lysate(54.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/csl-RBPJK-Antibody-4E12-Western-Blot-H00003516-M01-img0003.jpg "Western Blot: csl/RBPJK Antibody (4E12) [H00003516-M01] - Analysis of RBPJ expression in transfected 293T cell line by RBPJ monoclonal antibody (M01), clone 4E12.Lane 1: RBPJ transfected lysate(54.3 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: csl/RBPJK Antibody (4E12) [H00003516-M01] - RBPJ monoclonal antibody (M01), clone 4E12 Analysis of RBPJ expression in Jurkat.](http://images.novusbio.com/fullsize/csl-RBPJK-Antibody-4E12-Western-Blot-H00003516-M01-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: csl/RBPJK Antibody [NBP2-56071] - Staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/csl-RBPJK-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56071-img0001.jpg "Immunocytochemistry/Immunofluorescence: csl/RBPJK Antibody [NBP2-56071] - Staining of human cell line A-431 shows localization to nucleoplasm.")
![Western Blot: RBPJK Overexpression Lysate (Adult Normal) [NBP2-04570] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RBPJK.](http://images.novusbio.com/fullsize/RBPJK-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04570-img0001.jpg "Western Blot: RBPJK Overexpression Lysate (Adult Normal) [NBP2-04570] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RBPJK.")
![Western Blot: RBPJK Overexpression Lysate (Adult Normal) [NBL1-15233] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RBPJK.](http://images.novusbio.com/fullsize/RBPJK-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15233-img0002.jpg "Western Blot: RBPJK Overexpression Lysate (Adult Normal) [NBL1-15233] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RBPJK.")
![Western Blot: RBPJK Overexpression Lysate (Adult Normal) [NBL1-15231] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RBPJK.](http://images.novusbio.com/fullsize/RBPJK-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15231-img0002.jpg "Western Blot: RBPJK Overexpression Lysate (Adult Normal) [NBL1-15231] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RBPJK.")