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![Immunohistochemistry: MAD2L1 Antibody [NB100-563] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- MAD2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-NB100-563-img0006.jpg "Immunohistochemistry: MAD2L1 Antibody [NB100-563] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- MAD2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: MAD2L1 Antibody [NB100-563] - Detection of human MAD2L1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MAD2L1 antibody NB100-563 (lot 3) used for IP at 3 ug/mg lysate. MAD2L1 was also immunoprecipitated by a previous lot (lot 2) and by rabbit anti-MAD2L1 antibody NB100-564. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunoprecipitation-NB100-563-img0007.jpg)
![Western Blot: MAD2L1 Antibody [NBP1-31311] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Western-Blot-NBP1-31311-img0021.jpg "Western Blot: MAD2L1 Antibody [NBP1-31311] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: MAD2L1 Antibody [NBP1-31311] - Analysis of human osteosarcoma cell line U2OS, using MAD2L1 antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31311-img0008.jpg)
![Immunohistochemistry-Paraffin: MAD2L1 Antibody [NBP1-31311] - Human A549 xenograft . MAD2L1 antibody [C2C3], C-term diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-Paraffin-NBP1-31311-img0013.jpg)
![Western Blot: MAD2L1 Antibody [NBP1-31311] - A:Hep G2 B:MOLT4 C:Raji 12% SDS PAGE NBP1-31311 diluted at 1:1000](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Western-Blot-NBP1-31311-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MAD2L1 Antibody [NBP1-31311] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Western-Blot-NBP1-31311-img0019.jpg)
![Immunohistochemistry-Paraffin: MAD2L1 Antibody [NBP1-31311] - DLD-1 xenograft, using Mad2L1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-Paraffin-NBP1-31311-img0009.jpg)
![Immunohistochemistry-Paraffin: MAD2L1 Antibody [NBP1-31311] - Rat fore brain. MAD2L1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-Paraffin-NBP1-31311-img0012.jpg)
![Immunoprecipitation: MAD2L1 Antibody [NBP1-31311] - MAD2L1 protein from 293T whole cell extracts using 5 ug of MAD2L1 antibody [C2C3], C-term (NBP1-31311). Western blot analysis was performed using MAD2L1 antibody [C2C3], C-term (NBP1-31311). EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunoprecipitation-NBP1-31311-img0017.jpg)
![Western Blot: MAD2L1 Antibody [NBP1-83185] - Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Western-Blot-NBP1-83185-img0004.jpg "Western Blot: MAD2L1 Antibody [NBP1-83185] - Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: MAD2L1 Antibody [NBP1-83185] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83185-img0005.jpg)
![Immunohistochemistry-Paraffin: MAD2L1 Antibody [NBP1-83185] - Staining of human kidney shows strong cytoplasmic positivity in distal tubules.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-Paraffin-NBP1-83185-img0006.jpg)
![Western Blot: MAD2L1 Antibody (5W5U1) [NBP3-15384] - Western blot analysis of extracts of various cell lines, using MAD2/MAD2L1 Rabbit mAb (NBP3-15384) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-5W5U1-Western-Blot-NBP3-15384-img0003.jpg "Western Blot: MAD2L1 Antibody (5W5U1) [NBP3-15384] - Western blot analysis of extracts of various cell lines, using MAD2/MAD2L1 Rabbit mAb (NBP3-15384) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: MAD2L1 Antibody (5W5U1) [NBP3-15384] - Immunohistochemistry of paraffin-embedded mouse kidney using MAD2/MAD2L1 Rabbit mAb (NBP3-15384) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-5W5U1-Immunohistochemistry-Paraffin-NBP3-15384-img0002.jpg)
![Immunohistochemistry-Paraffin: MAD2L1 Antibody (5W5U1) [NBP3-15384] - Immunohistochemistry of paraffin-embedded rat spleen using MAD2/MAD2L1 Rabbit mAb (NBP3-15384) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-5W5U1-Immunohistochemistry-Paraffin-NBP3-15384-img0001.jpg)
![Western Blot: Mad2L1 Antibody (4D2) [NBP2-02103] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Mad2L1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Mad2L1.](http://images.novusbio.com/fullsize/Mad2L1-Antibody-4D2-Western-Blot-NBP2-02103-img0001.jpg "Western Blot: Mad2L1 Antibody (4D2) [NBP2-02103] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Mad2L1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Mad2L1.")
![Immunocytochemistry/Immunofluorescence: Mad2L1 Antibody (4D2) [NBP2-02103] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Mad2L1.](http://images.novusbio.com/fullsize/Mad2L1-Antibody-4D2-Immunocytochemistry-Immunofluorescence-NBP2-02103-img0002.jpg)
![Western Blot: Mad2L1 Antibody [NB300-880] - Analysis of Mad2L1 in HEK293 lysate (35ug protein in RIPA buffer) using NB300-880 at 2ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Mad2L1-Antibody-Western-Blot-NB300-880-img0002.jpg "Western Blot: Mad2L1 Antibody [NB300-880] - Analysis of Mad2L1 in HEK293 lysate (35ug protein in RIPA buffer) using NB300-880 at 2ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: MAD2L1 Antibody (1761) [NBP2-43820] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [1761].](http://images.novusbio.com/fullsize/MAD2L1-Antibody-1761-Western-Blot-NBP2-43820-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: MAD2L1 Antibody (1761) [NBP2-43820] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [1761].")
![<b>Genetic Strategies Validation. </b>Western Blot: MAD2L1 Antibody (1761) [NBP2-43820] - Analysis of A. 30 ug Jurkat whole cell lysate/extract B. 30 ug Raji whole cell lysate/extract 12 % SDS-PAGE MAD2L1 antibody [1761] dilution: 1:250.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-1761-Western-Blot-NBP2-43820-img0001.jpg)
![Western Blot: MAD2L1 Antibody [NBP1-33529] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [N1C3].](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Western-Blot-NBP1-33529-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: MAD2L1 Antibody [NBP1-33529] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [N1C3].")
![<b>Genetic Strategies Validation. </b>Western Blot: MAD2L1 Antibody [NBP1-33529] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Western-Blot-NBP1-33529-img0004.jpg)
![Western Blot: Mad2L1 Overexpression Lysate (Adult Normal) [NBL1-12786] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Mad2L1.](http://images.novusbio.com/fullsize/Mad2L1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12786-img0002.jpg "Western Blot: Mad2L1 Overexpression Lysate (Adult Normal) [NBL1-12786] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Mad2L1.")
![Recombinant Human MAD2L1 Protein [NBP1-72458]](http://images.novusbio.com/fullsize/Recombinant-Human-MAD2L1-Protein-NBP1-72458-img0001.jpg "Recombinant Human MAD2L1 Protein [NBP1-72458]")