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MAD2L1 Antibody (OTI4D2) - Azide and BSA Free

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Western Blot: MAD2L1 Antibody (OTI4D2) - Azide and BSA Free [NBP2-72565] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Mad2L1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Mad2L1.
Western Blot: MAD2L1 Antibody (OTI4D2) - Azide and BSA Free [NBP2-72565] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Mad2L1 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: MAD2L1 Antibody (OTI4D2) - Azide and BSA Free [NBP2-72565] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Mad2L1.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clone
OTI4D2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-72565AF350
Alexa Fluor 405
NBP2-72565AF405
Alexa Fluor 488
NBP2-72565AF488
Alexa Fluor 532
NBP2-72565AF532
Alexa Fluor 594
NBP2-72565AF594
Alexa Fluor 647
NBP2-72565AF647
Alexa Fluor 700
NBP2-72565AF700
Alexa Fluor 750
NBP2-72565AF750
Allophycocyanin
NBP2-72565APC
Biotin
NBP2-72565B
CoraFluor 1
NBP2-72565CL1
DyLight 350
NBP2-72565UV
DyLight 405
NBP2-72565V
DyLight 488
NBP2-72565G
DyLight 550
NBP2-72565R
DyLight 594
NBP2-72565DL594
DyLight 650
NBP2-72565C
DyLight 680
NBP2-72565FR
DyLight 755
NBP2-72565IR
FITC
NBP2-72565F
HRP
NBP2-72565H
Janelia Fluor 525
NBP2-72565JF525
Janelia Fluor 549
NBP2-72565JF549
Janelia Fluor 646
NBP2-72565JF646
Janelia Fluor 669
NBP2-72565JF669
PE
NBP2-72565PE
PerCP
NBP2-72565PCP
Unconjugated
NBP2-02103
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-02103
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MAD2L1 Antibody (OTI4D2) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human MAD2L1 (NP_002349) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
MAD2L1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
Theoretical MW
23.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for MAD2L1 Antibody (OTI4D2) - Azide and BSA Free

  • HSMAD2
  • MAD2 (mitotic arrest deficient, yeast, homolog)-like 1
  • MAD2 mitotic arrest deficient-like 1 (yeast)
  • MAD2L1
  • MAD2-like protein 1
  • MAD2mitotic arrest deficient, yeast, homolog-like 1
  • Mitotic arrest deficient 2-like protein 1
  • mitotic spindle assembly checkpoint protein MAD2A

Background

The spindle checkpoint ensures the fidelity of chromosome segregation in mitosis and meiosis. In response to defects in the mitotic apparatus, it blocks the activity of the anaphase-promoting complex, a large ubiquitin ligase required for chromosome segregation. Recent studies indicate that the spindle checkpoint monitors both the attachment of chromosomes to the mitotic spindle and the tension across the sister chromatid generated by microtubules. Upon checkpoint activation, checkpoint protein complexes containing BubR1(Mad3), Bub3, Mad2 and Cdc20 directly bind to the anaphase-promoting complex and inhibit its ligase activity. Therefore, the checkpoint proteins form a complex intracellular signalling network to inhibit the anaphase-promoting complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MAD2L1