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MAD2L1 Antibody

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Western Blot: Mad2L1 Antibody [NB300-880] - Analysis of Mad2L1 in HEK293 lysate (35ug protein in RIPA buffer) using NB300-880 at 2ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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MAD2L1 Antibody Summary

Immunogen
Peptide with sequence C-KVNSMVAYKIPVND corresponding to C-Terminus according to NP_002349.1.
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MAD2L1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 2 - 4 ug/ml
Application Notes
WB: Approx. 24 kDa band observed in HEK293 lysates (calculated MW of 23.5 kDa band according to NP_002349.1). An additional band of unknown identity was also consistently observed at approx. 29 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Control
MAD2L1 Overexpression Lysate
Publications
Read Publication using NB300-880.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MAD2L1 Antibody

  • HSMAD2
  • MAD2 (mitotic arrest deficient, yeast, homolog)-like 1
  • MAD2 mitotic arrest deficient-like 1 (yeast)
  • MAD2L1
  • MAD2-like protein 1
  • MAD2mitotic arrest deficient, yeast, homolog-like 1
  • Mitotic arrest deficient 2-like protein 1
  • mitotic spindle assembly checkpoint protein MAD2A

Background

MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MAD2L1 Antibody (NB300-880)(1)


Showing Publication 1 - 1 of 1.
Publication using NB300-880 Applications Species
Li Y, Benezra R. Identification of a human mitotic checkpoint gene: hsMAD2. Science 1996-10-11 [PMID: 8824189]

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAD2L1
Entrez
Uniprot