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![Western Blot: nSMase Antibody [NBP1-87810] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/nSMase-Antibody-Western-Blot-NBP1-87810-img0004.jpg "Western Blot: nSMase Antibody [NBP1-87810] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![Immunocytochemistry/Immunofluorescence: nSMase Antibody [NBP1-87810] - Staining of human cell line U-2 OS shows localization to plasma membrane & vesicles. Antibody staining shown in green.](http://images.novusbio.com/fullsize/nSMase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87810-img0006.jpg)
![Immunohistochemistry-Paraffin: nSMase Antibody [NBP1-87810] - Staining of human tonsil shows strong membranous positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/nSMase-Antibody-Immunohistochemistry-Paraffin-NBP1-87810-img0011.jpg)
![Immunohistochemistry-Paraffin: nSMase Antibody [NBP1-87810] - Staining of human Fallopian tube shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/nSMase-Antibody-Immunohistochemistry-Paraffin-NBP1-87810-img0008.jpg)
![Immunohistochemistry-Paraffin: nSMase Antibody [NBP1-87810] - Staining of human skeletal muscle shows very weak positivity in myocytes.](http://images.novusbio.com/fullsize/nSMase-Antibody-Immunohistochemistry-Paraffin-NBP1-87810-img0009.jpg)
![Immunohistochemistry-Paraffin: nSMase Antibody [NBP1-87810] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/nSMase-Antibody-Immunohistochemistry-Paraffin-NBP1-87810-img0010.jpg)
![Immunohistochemistry-Paraffin: nSMase Antibody (2C9) [H00006610-M01] - Analysis of monoclonal antibody to SMPD2 on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/nSMase-Antibody-2C9-Immunohistochemistry-Paraffin-H00006610-M01-img0004.jpg "Immunohistochemistry-Paraffin: nSMase Antibody (2C9) [H00006610-M01] - Analysis of monoclonal antibody to SMPD2 on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml.")
![Sandwich ELISA: nSMase Antibody (2C9) [H00006610-M01] - Detection limit for recombinant GST tagged SMPD2 is approximately 10ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/nSMase-Antibody-2C9-Sandwich-ELISA-H00006610-M01-img0005.jpg)
![Western Blot: nSMase Antibody [NBP3-35179] - Western blot analysis of various lysates using nSMase Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35179_rabbit-nsmase-pab-912202415143643.jpg "Western Blot: nSMase Antibody [NBP3-35179] - Western blot analysis of various lysates using nSMase Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Western Blot: nSMase Antibody [NBP1-59937] - HepG2 cell lysate, concentration 1.25ug/ml.](http://images.novusbio.com/fullsize/nSMase-Antibody-Western-Blot-NBP1-59937-img0003.jpg "Western Blot: nSMase Antibody [NBP1-59937] - HepG2 cell lysate, concentration 1.25ug/ml.")
![Western Blot: nSMase Antibody [NBP1-59936] - Titration: 0.2-1 ug/ml, Positive Control: COLO205 cell lysate.](http://images.novusbio.com/fullsize/nSMase-Antibody-Western-Blot-NBP1-59936-img0003.jpg "Western Blot: nSMase Antibody [NBP1-59936] - Titration: 0.2-1 ug/ml, Positive Control: COLO205 cell lysate.")
![Western Blot: nSMase Overexpression Lysate (Adult Normal) [NBP2-10865] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for nSMase.](http://images.novusbio.com/fullsize/nSMase-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10865-img0001.jpg "Western Blot: nSMase Overexpression Lysate (Adult Normal) [NBP2-10865] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for nSMase.")
![Immunohistochemistry-Paraffin: nSMase Antibody [NBP2-97998] - Immunochemical staining of human nSMase in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nSMase-Antibody-Immunohistochemistry-Paraffin-NBP2-97998-img0001.jpg "Immunohistochemistry-Paraffin: nSMase Antibody [NBP2-97998] - Immunochemical staining of human nSMase in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![ELISA: Human nSMase - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39157] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39157_human-nsmase-ready-to-use-elisa-kit-colorimetric-2110202415384010.png "ELISA: Human nSMase - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39157] - Standard Curve Reference")
![ELISA: Human nSMase ELISA Kit (Colorimetric) [NBP3-39156] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39156_human-nsmase-elisa-kit-colorimetric-2110202415371310.png "ELISA: Human nSMase ELISA Kit (Colorimetric) [NBP3-39156] - Standard Curve Reference")