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![Knockdown Validated: DDIT4 Antibody [NBP1-77321] - siRNA knockdown of the DDIT4 antiproliferative responses to 1,25(OH)2 D3RMCs were transfected with negative control siRNA orDDIT4-specific siRNA in the presence or absence of 1,25(OH) 2 D3 . (C) DDIT4 protein expression levels were detected by Western blot. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.23063) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DDIT4-Antibody-Knockdown-Validated-NBP1-77321-img0009.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: DDIT4 Antibody [NBP1-77321] - siRNA knockdown of the DDIT4 antiproliferative responses to 1,25(OH)2 D3RMCs were transfected with negative control siRNA orDDIT4-specific siRNA in the presence or absence of 1,25(OH) 2 D3 . (C) DDIT4 protein expression levels were detected by Western blot. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.23063) licensed under a CC-BY license.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: DDIT4 Antibody [NBP1-77321] - 1,25(OH)2 D3 modulates RMCs proliferation induced by high glucose via the DDIT4/mTOR signaling pathwayRMCs were cultured for 48 h in the presence or absence of 1,25(OH)2 D3. Cell lysates were subjected to Western blot analysis to measure the levels of DDIT4. LG: 5.5 mM glucose; Man: 5.5 mM glucose + 24.5 mM mannitol; HG: 30 mM glucose. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.23063) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DDIT4-Antibody-Western-Blot-NBP1-77321-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: DDIT4 Antibody [NBP1-77321] - Mouse kidney tissue with NBP1-77321 antibody at 20 ug/mL. Red: Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/DDIT4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77321-img0008.jpg)
![Immunohistochemistry-Paraffin: DDIT4 Antibody [NBP1-77321] - Mouse kidney tissue with DDIT4 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/DDIT4-Antibody-Immunohistochemistry-Paraffin-NBP1-77321-img0003.jpg)
![Western Blot: DDIT4 Antibody [NBP1-77321] - Human kidney tissue lysate with DDIT4 antibody at (A) 0.5 and (B) 1 ug/mL.](http://images.novusbio.com/fullsize/DDIT4-Antibody-Western-Blot-NBP1-77321-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: DDIT4 Antibody - BSA Free [NBP1-77321] - Immunofluorescence of DDIT4 in mouse kidney tissue with DDIT4 antibody at 20 u/mL.Red: DDIT4 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-77321_rabbit-polyclonal-ddit4-antibody-2711202419592030.gif)
![Immunohistochemistry: DDIT4 Antibody - BSA Free [NBP1-77321] - Immunohistochemistry of DDIT4 in mouse kidney tissue with DDIT4 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77321_rabbit-polyclonal-ddit4-antibody-2711202420432384.jpg)
![Immunohistochemistry: DDIT4 Antibody - BSA Free [NBP1-77321] - Immunohistochemistry of DDIT4 in mouse kidney tissue with DDIT4 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77321_rabbit-polyclonal-ddit4-antibody-271120242016958.jpg)
![Immunocytochemistry/ Immunofluorescence: DDIT4 Antibody - BSA Free [NBP1-77321] - Immunofluorescence of DDIT4 in mouse kidney tissue with DDIT4 antibody at 20 u/mL.Red: DDIT4 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-77321_rabbit-polyclonal-ddit4-antibody-2711202420243345.gif)
![Western Blot: DDIT4 Antibody [NBP1-22966] - Influenza virus down-regulates REDD1 to promote mTORC1 activity. A549 cells were infected with WSN at MOI of 2 PFU/cell for the indicated times. A549 cells were transfected with siRNAs (pool of three each) targeting viral mRNAs and then infected for 7 h at MOI of 2 PFU/cell. Immunoblot analysis was performed to detect the depicted proteins, n = 3. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1006635), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DDIT4-Antibody-Western-Blot-NBP1-22966-img0005.jpg "Western Blot: DDIT4 Antibody [NBP1-22966] - Influenza virus down-regulates REDD1 to promote mTORC1 activity. A549 cells were infected with WSN at MOI of 2 PFU/cell for the indicated times. A549 cells were transfected with siRNAs (pool of three each) targeting viral mRNAs and then infected for 7 h at MOI of 2 PFU/cell. Immunoblot analysis was performed to detect the depicted proteins, n = 3. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1006635), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: DDIT4 Antibody [NBP1-22966] - HeLa cells stained with NBP1-22966 after 0.2% hypoxia or CoCl2 overnight. Image from verified customer review.](http://images.novusbio.com/fullsize/DDIT4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-22966-img0004.jpg)
![Western Blot: DDIT4 Antibody [NBP1-22966] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Lysate was prepared from cells that had been treated with cobalt chloride (A and B) or mock treated (A). NBP1-22966 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate.](http://images.novusbio.com/fullsize/DDIT4-Antibody-Western-Blot-NBP1-22966-img0002.jpg)
![Western Blot: DDIT4 Antibody [NBP1-22966] - Influenza virus down-regulates REDD1 to promote mTORC1 activity.(A-C) A549 cells were infected with WSN at MOI of 2 PFU/cell for the indicated times. A549 cells were transfected with siRNAs (pool of three each) targeting viral mRNAs and then infected for 7 h at MOI of 2 PFU/cell. Immunoblot analysis was performed to detect the depicted proteins, n = 3. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1006635), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DDIT4-Antibody-Western-Blot-NBP1-22966-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: DDIT4 Antibody [NBP1-22966] - NBF-fixed asynchronous, cobalt-treated HeLa cells. Antibody: Affinity purified rabbit anti-REDD1 used at a dilution of 1:500 (2 ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG H&L crossadsorbed Antibody DyLight®594 used at 1:100.](http://images.novusbio.com/fullsize/DDIT4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-22966-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: DDIT4 Antibody [NBP1-85211] - Staining of human cell line hTCEpi shows localization to cytosol.](http://images.novusbio.com/fullsize/DDIT4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85211-img0004.jpg "Immunocytochemistry/Immunofluorescence: DDIT4 Antibody [NBP1-85211] - Staining of human cell line hTCEpi shows localization to cytosol.")
![Immunohistochemistry-Paraffin: DDIT4 Antibody [NBP1-85211] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/DDIT4-Antibody-Immunohistochemistry-Paraffin-NBP1-85211-img0002.jpg)
![Western Blot: DDIT4 Antibody [NBP2-92942] - Western blot analysis of extracts of various cell lines, using DDIT4 antibody (NBP2-92942) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/DDIT4 Antibody-Western Blot-NBP2-92942-img0004.jpg "Western Blot: DDIT4 Antibody [NBP2-92942] - Western blot analysis of extracts of various cell lines, using DDIT4 antibody (NBP2-92942) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunocytochemistry/Immunofluorescence: DDIT4 Antibody [NBP2-92942] - Immunofluorescence analysis of PC-12 cells using [KO Validated] DDIT4 Rabbit pAb (NBP2-92942) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DDIT4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92942-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: DDIT4 Antibody [NBP2-92942] - Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] DDIT4 Rabbit pAb (NBP2-92942) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DDIT4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92942-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: DDIT4 Antibody [NBP2-92942] - Analysis of extracts from normal (control) and DDIT4 knockout (KO) HeLa cells, using DDIT4 .Exposure time: 90s.](http://images.novusbio.com/fullsize/DDIT4-Antibody-Knockout-Validated-NBP2-92942-img0001.jpg)
![Western Blot: DDIT4 Antibody [NBP2-38208] - Analysis in control (vector only transfected HEK293T lysate) and DDIT4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/DDIT4-Antibody-Western-Blot-NBP2-38208-img0003.jpg "Western Blot: DDIT4 Antibody [NBP2-38208] - Analysis in control (vector only transfected HEK293T lysate) and DDIT4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: DDIT4 Antibody [NBP2-38208] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/DDIT4-Antibody-Immunohistochemistry-Paraffin-NBP2-38208-img0001.jpg)
![Western Blot: DDIT4 [p Thr23, p Thr25] Antibody [NBP2-29521] - Western blot of human jurkat lysate showing specific immunolabeling of the ~34k REDD1 protein phosphorylated at Thr23/25 (control). Immunolabeling is blocked by the phospho-peptide used as antigen (peptide) but not by the corresponding dephospho-peptide (not shown).](http://images.novusbio.com/fullsize/DDIT4-[p-Thr23-p-Thr25]-Antibody-Western-Blot-NBP2-29521-img0001.jpg "Western Blot: DDIT4 [p Thr23, p Thr25] Antibody [NBP2-29521] - Western blot of human jurkat lysate showing specific immunolabeling of the ~34k REDD1 protein phosphorylated at Thr23/25 (control). Immunolabeling is blocked by the phospho-peptide used as antigen (peptide) but not by the corresponding dephospho-peptide (not shown).")
![Western Blot: DDIT4 Overexpression Lysate (Adult Normal) [NBL1-09773] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DDIT4.](http://images.novusbio.com/fullsize/DDIT4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09773-img0002.jpg "Western Blot: DDIT4 Overexpression Lysate (Adult Normal) [NBL1-09773] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DDIT4.")
![Western Blot: DDIT4 Antibody (PS00-10) [NBP3-32259] - Western blot analysis of DDIT4 on different lysates with Rabbit anti-DDIT4 antibody (NBP3-32259) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 0.5mM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 25 kDa Observed band size: 30 kDa Exposure time: 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32259) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32259_rabbit-ddit4-mab-ps00-10-278202416285225.jpg "Western Blot: DDIT4 Antibody (PS00-10) [NBP3-32259] - Western blot analysis of DDIT4 on different lysates with Rabbit anti-DDIT4 antibody (NBP3-32259) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 0.5mM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 25 kDa Observed band size: 30 kDa Exposure time: 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32259) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![SDS-Page: Recombinant Human DDIT4 Protein [NBP1-50976] - 15 % SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-DDIT4-Protein-SDS-Page-NBP1-50976-img0001.jpg "SDS-Page: Recombinant Human DDIT4 Protein [NBP1-50976] - 15 % SDS-PAGE (3ug)")