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![Western Blot: SIRT4 Antibody [NB100-1406] - Immunoblots reveal the levels of sirtuins (SIRT4-7) in the OSCC cell lines HSC-3, UM-SCC-1, and UM-SCC-17B and in normal human oral keratinocytes (K). B-Actin served as loading control. Antibodies include SIRT4 (NB100-1406), SIRT5 (NB100-56372), and SIRT7 (NB100-56364). Image collected and cropped by CiteAb from the following publication (doi.org/10.1002/cncr.25676) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SIRT4-Antibody-Western-Blot-NB100-1406-img0004.jpg "Western Blot: SIRT4 Antibody [NB100-1406] - Immunoblots reveal the levels of sirtuins (SIRT4-7) in the OSCC cell lines HSC-3, UM-SCC-1, and UM-SCC-17B and in normal human oral keratinocytes (K). B-Actin served as loading control. Antibodies include SIRT4 (NB100-1406), SIRT5 (NB100-56372), and SIRT7 (NB100-56364). Image collected and cropped by CiteAb from the following publication (doi.org/10.1002/cncr.25676) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: SIRT4 Antibody [NB100-1406] - Staining of paraffin embedded Human Skeletal Muscle. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/SIRT4-Antibody-Immunohistochemistry-Paraffin-NB100-1406-img0001.jpg)
![Western Blot: SIRT4 Antibody [NB100-1406] - Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/SIRT4-Antibody-Western-Blot-NB100-1406-img0003.jpg)
![Western Blot: SIRT4 Antibody [NBP1-80746] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/SIRT4-Antibody-Western-Blot-NBP1-80746-img0004.jpg "Western Blot: SIRT4 Antibody [NBP1-80746] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SIRT4 Antibody [NBP1-80746] - Staining in human testis and pancreas tissues using anti-SIRT4 antibody. Corresponding SIRT4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SIRT4-Antibody-Immunohistochemistry-Paraffin-NBP1-80746-img0007.jpg)
![Immunohistochemistry-Paraffin: SIRT4 Antibody [NBP1-80746] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/SIRT4-Antibody-Immunohistochemistry-Paraffin-NBP1-80746-img0005.jpg)
![Immunohistochemistry-Paraffin: SIRT4 Antibody [NBP1-80746] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/SIRT4-Antibody-Immunohistochemistry-Paraffin-NBP1-80746-img0006.jpg)
![Western Blot: SIRT4 Antibody [NBP1-80002] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:62500 Positive Control: MCF7 cell lysate](http://images.novusbio.com/fullsize/SIRT4-Antibody-Western-Blot-NBP1-80002-img0010.jpg "Western Blot: SIRT4 Antibody [NBP1-80002] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:62500 Positive Control: MCF7 cell lysate")
![Immunohistochemistry: SIRT4 Antibody [NBP1-80002] - Staining of a pancreas crossection.](http://images.novusbio.com/fullsize/SIRT4-Antibody-Immunohistochemistry-NBP1-80002-img0008.jpg)
![Western Blot: SIRT4 Antibody [NBP1-80002] - Antibody Titration: 1 ug/ml Human Liver.](http://images.novusbio.com/fullsize/SIRT4-Antibody-Western-Blot-NBP1-80002-img0005.jpg)
![Western Blot: SIRT4 Antibody [NBP1-80002] - MCF7 Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1 ug/ml Peptide Concentration: 5 ug/ml lysate Quantity: 25ug/ Lane/ Lane Gel Concentration: 0.12.](http://images.novusbio.com/fullsize/SIRT4-Antibody-Western-Blot-NBP1-80002-img0007.jpg)
![Western Blot: SIRT4 Antibody [NBP1-80002] - Antibody Titration: 1 ug/ml Human Raji.](http://images.novusbio.com/fullsize/SIRT4-Antibody-Western-Blot-NBP1-80002-img0006.jpg)
![Western Blot: SIRT4 Antibody [NBP1-80002] - Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/SIRT4-Antibody-Western-Blot-NBP1-80002-img0009.jpg)
![Western Blot: SIRT4 Antibody [NBP1-80747] - Analysis in human cell line HEK 293.](http://images.novusbio.com/fullsize/SIRT4-Antibody-Western-Blot-NBP1-80747-img0005.jpg "Western Blot: SIRT4 Antibody [NBP1-80747] - Analysis in human cell line HEK 293.")
![Immunohistochemistry-Paraffin: SIRT4 Antibody [NBP1-80747] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/SIRT4-Antibody-Immunohistochemistry-Paraffin-NBP1-80747-img0003.jpg)
![Western Blot: SIRT4 Antibody [NBP1-80747] - Analysis in control (vector only transfected HEK293T lysate) and SIRT4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/SIRT4-Antibody-Western-Blot-NBP1-80747-img0006.jpg)
![Western Blot: SIRT4 Antibody (1C8) [H00023409-M03] - Analysis of SIRT4 expression in Raw 264.7.](http://images.novusbio.com/fullsize/SIRT4-Antibody-1C8-Western-Blot-H00023409-M03-img0003.jpg "Western Blot: SIRT4 Antibody (1C8) [H00023409-M03] - Analysis of SIRT4 expression in Raw 264.7.")
![ELISA: SIRT4 Antibody (1C8) [H00023409-M03] - Detection limit for recombinant GST tagged SIRT4 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SIRT4-Antibody-1C8-ELISA-H00023409-M03-img0004.jpg)
![Western Blot: SIRT4 Antibody [NBP2-98856] - Lane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDaObserved band size:40 kDa](http://images.novusbio.com/fullsize/SIRT4-Antibody-Western-Blot-NBP2-98856-img0002.jpg "Western Blot: SIRT4 Antibody [NBP2-98856] - Lane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDaObserved band size:40 kDa")
![Immunoprecipitation: SIRT4 Antibody [NBP2-98856] - Lane A:0.5 mg Jurkat Whole Cell Lysate0.5 uL anti-SIRT4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-SIRT4 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 35 kDa](http://images.novusbio.com/fullsize/SIRT4-Antibody-Immunoprecipitation-NBP2-98856-img0001.jpg)
![Western Blot: SIRT4 Overexpression Lysate (Adult Normal) [NBL1-15977] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SIRT4.](http://images.novusbio.com/fullsize/SIRT4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15977-img0002.jpg "Western Blot: SIRT4 Overexpression Lysate (Adult Normal) [NBL1-15977] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SIRT4.")
![ELISA: Human SIRT4 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40533] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40533_human-sirt4-ready-to-use-elisa-kit-colorimetric-211020241526432.png "ELISA: Human SIRT4 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40533] - Standard Curve Reference")
![ELISA: Human SIRT4 ELISA Kit (Colorimetric) [NBP3-40534] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40534_human-sirt4-elisa-kit-colorimetric-2110202415351211.png "ELISA: Human SIRT4 ELISA Kit (Colorimetric) [NBP3-40534] - Standard Curve Reference")