Immunocytochemistry

Kristy R. Howell, PhD

Why: To identify a specific organelle or another cellular structure and to mark individual cells, it is necessary to counterstain them in immunocytochemistry/immunofluorescence (ICC/IF) assays.

The human form of microtubule-associated protein light chain 3 (LC3) is expressed as 3 splice variants LC3A, LC3B, and LC3C.¹ LC3B is a subunit of the MAP1A and MAP1B microtubule-binding proteins and plays a central role in autophagosome membrane structure.

Immunocytochemistry (ICC) is a frequently used technique that allows researchers to determine if an antigen is expressed by specific cellular or sub-cellular compartments via the use of antibodies. Many research-grade antibodies are suitable for ICC analysis, however many are not or have not been tested.