Newly Validated Antibodies for Immunocytochemistry

Immunocytochemistry (ICC) is a frequently used technique that allows researchers to determine if an antigen is expressed by specific cellular or sub-cellular compartments via the use of antibodies. Many research-grade antibodies are suitable for ICC analysis, however many are not or have not been tested.

Our antibody catalog includes over 1,800 antibodies that have been tested for use in ICC analysis. We at Novus Biologicals recently validated 25 of our antibodies for use in ICC. This ICC testing was conducted on either HeLa cell extracts or NTERA-2 cells. HeLa is an immortal, cervical cancer cell line that is frequently used in scientific research, and NTERA-2 is a human neural cell line that is commonly used as well.

One of our Endoplasmic Reticulum Marker antibodies, an anti-PDI antibody (catalog number NB100-1921), was validated for use in ICC by staining in HeLa cells. One of our Embryonic Stem Cell Marker antibodies, an anti-Cripto 1 antibody (catalog number NB100-1598), was validated for use in ICC by staining both HeLa cells and NTERA-2 cells, both of which were detected with a DyLight 488 labeled secondary antibody and can be viewed here.

Immunocytochemistry/Immunofluorescence: PDI Antibody [NB100-1921

In addition to the 25 newly characterized antibodies, all ICC tested antibodies can be viewed on our Immunocytochemical Antibodies page. We at Novus Biologicals are dedicated to providing high quality and well validated antibodies, therefore our Antibody Lab Team will continue to test our antibodies for use in a variety of applications including ICC.