HSP60 antibody

I began using the HSP60 antibody (NB110-57063) in June of 2010 and it worked well. I do not like to buy antibodies that have not been tested in the species for which I will use them, so I picked this antibody because it had already been tested in rat tissue. I split the antibody into 20ul aliquots and stored it at -20C. I first ran a Western blot with 15ug of a RIPA whole cell lystate from WKPT cells a rat kidney immortalized cell line derived from the S1 proximal tubule segment.

Heat Shock Proteins (HSPs) are a ubiquitous group of molecular chaperone proteins that have evolved unique mechanisms, within their host cells, to facilitate survival in hostile environments such as heat, oxidative (hypoxia), pH and cold.

Novus Biologicals offers an extensive antibody catalog targeting heat shock proteins (HSPs). A large protein group covering a number of families, the HSPs are functionally related by their dramatic upregulation in response to stress. Stress triggers may include a rise in temperature or a similar environmental cause. Transcription is controlled by the heat shock factor, or HSF, protein family.