Antibody News

Tumor Necrosis Factor alpha (TNFa) is a cytokine protein that plays an essential role in inducing the systemic inflammatory response, being expressed in tissues throughout the body. In autoimmune diseases such as rheumatoid arthritis and psoriasis, TNF alpha causes negative clinical effects. These can be controlled by inhibitory TNF antibody drugs, a number of which are now on the market. We at Novus Biologicals have an extensive range of TNF alpha antibodies.

TNF alpha is expressed by a number of cell types, primarily macrophages. TNF antibody research has shown the protein to have a number of effects, both positive and negative. In mice, it causes tumor necrosis when injected into tumor sites, and studies have shown it can inhibit tumor growth, induce apoptosis, and inhibit...

The Hypoxia-inducible Factor 1 (HIF1) protein is a heterodimeric transcription factor which plays an important role in mammalian oxygen homeostasis in conditions of hypoxia, or low oxygen concentration. HIF-1 alpha antibody reagents are widely used in disease research, as hypoxia is known to contribute to many diseases including cancer, pulmonary hypertension and congenital heart disease. We at Novus Biologicals are one of the leading antibody suppliers of HIF-1 products.

HIF-1 alpha is one of two subunits forming the HIF-1 heterodimer. Under normal conditions it undergoes continual translation, ubiquitination and degradation, but in hypoxic conditions degradation ceases while transcription increases. Expression of HIF-1 alpha is regulated by several enzymes, including the E3 ubiquitin-protein ligase Siah2.

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Nestin is a large class Vl intermediate filament protein predominantly expressed in the neuroepithelial stem cells of the embryonic central nervous system, although recent nestin antibody studies have shown it expressed in other cell types, including vascular endothelial cells. The first monoclonal nestin antibody was developed by Hockfield and McKay in 1985.

Nestin is widely used as a marker for stem, progenitor, glioma and tumor endothelial cells, and has proven to be a valuable angiogenic marker in tumor studies. It is widely expressed in glioblasta multiformes and primary and metastatic melanomas, though not metastatic carcinomas.

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We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid residues 95-108 of the simian virus 5 RNA polymerase alpha subunit.

Epitope tagging is widely used in proteomics and genetic studies. The epitope is, of course, the portion of the antigen protein to which the antibody binds. An epitope tag is an artificial epitope that is recognized by a common antibody. The genetic sequence of the tag is engineered using recombinant DNA techniques, and grafted into the protein of interest by placing the encoded sequence of the epitope into a matching open reading frame. The antibody raised...