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Laminin Biologically Active Protein

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    • Catalog Number
      NBP1-78301
    • Availability
      Product Discontinued

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Laminin Biologically Active Protein Summary

Description
A biologically active protein corresponding to LAMA1.
Localization
Secreted > extracellular space > extracellular matrix > basement membrane. Note: Major component.
Protein/Peptide Type
Biologically Active Protein
Gene
LAMA1
Purity
Protein A or G purified

Applications/Dilutions

Dilutions
  • Functional
  • Immunocytochemistry/ Immunofluorescence
  • Tissue Culture Substratum
Application Notes
Laminin promotes and may be required for cell adhesion, growth and motility of numerous cell types, especially embryonic and neural cells. For cell adhesion apply to tissue culture surfaces at 2-10 ug/ml. For neuritic growth apply to tissue culture surfaces (best when pretreated with polylysine or polyornithine) at 200-1000 ng/ml. Thaw product slowly at 2 to 8 degrees C. Do not refreeze. Once thawed, retain sterility and may be stored at 2 to 8 degrees C for up to 3 months.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-78301 in the following applications:
Publications
Read Publications using
NBP1-78301 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26801212)

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sterile liquid solution in phosphate buffered saline, pH 7.2 containing 0.1 mM EDTA. Found negative for bacteria, fungi, and mycoplasma
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Notes

Preperation: Laminin-1 was purified from mouse EHS sarcoma by combining methods of Paulsson (Eur J Biochem, 166: 11-19, 1987) and Engvall (Arch Biochem Biophys, 222: 649-656, 1983). The goal was to obtain a laminin preparation with high specific activity (for cell attachment and neuritic growth) and minimal growth factor contamination. All laminin fractions were selected based on immunoreactivity (ELISA) and bioassays for neurite-promoting and neurotrophic activities. Briefly, mouse tumor tissue was homogenized in the presence of a protease inhibitor cocktail (without metal chelators). The insoluble fraction was collected. Laminin-nidogen complex was then extracted by 10 mM EDTA chelation. The EDTA extract was fractionated by CL6B gel filtration. The highly enriched laminin fraction was further purified by heparin-affinity. The high specific activity laminin prep was concentrated and further purified by 100 kD ultrafiltration and flushing in the presence of EDTA. The prep was sterilized by the addition of 0.55% chloroform and then dialyzed exhaustively with PBS containing 0.1 EDTA using aseptic conditions. The resulting laminin-1 preparation is >95% pure showing the characteristic 200 and 400 kD bands by reducing SDS-PAGE. The major contaminant is the laminin-binding protein, nidogen. The preparation has high specific activity for promoting neurite growth in vitro (e.g., half-maximal=50 ng/ml and maximal=200 ng/ml for chick E8 dorsal root ganglionic neurons). Maximal cell attachment of Schwann cells occurs at less than or equal to 2 ug/ml. Applied to a tissue culture substratum the laminin preparation shows negligible neuron survival promoting activity.

Alternate Names for Laminin Biologically Active Protein

  • LAMA
  • Laminin A chain
  • laminin subunit alpha-1
  • laminin, alpha 1
  • Laminin-1 subunit alpha
  • Laminin-3 subunit alpha
  • PTBHS
  • S-LAM alpha
  • S-laminin subunit alpha

Background

Laminin, the most abundant structural and biologically active component in basement membranes, is a complex extracellular glycoprotein with high molecular weight (900 kDa). Laminin is composed of a laminin alpha (400 kDa), beta (215 kDa) and gamma chain (205 kDa), whose multidomain proteins are encoded by distinct genes (LAMA, LAMB, LAMC respectively) and have several isoforms of each chain. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, reflecting diverse functions in vivo. Laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Laminins are an important and biologically active part of the basal lamina, influencing cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis, where anti-laminin antibodies can be widely used to label blood vessels and basement membranes (1). Significant quantities of laminin are found in basement membranes, the thin extracellular matrices that surround epithelial tissue, nerve, fat cells and smooth, striated and cardiac muscle. Excessive serum laminin levels have been associated with fibrosis, cirrhosis and hepatitis, serious and frequent complications of chronic active liver disease characterized by excessive deposition of various normal components of connective tissue in liver (2). Epithelial mesenchymal transition (EMT) biomarkers include fibronectin, laminin, N-cadherin, and Slug (3).

References

1. Yang, M. Y., Chiao, M. T., Lee, H. T., Chen, C. M., Yang, Y. C., Shen, C. C., & Ma, H. I. (2015). An innovative three-dimensional gelatin foam culture system for improved study of glioblastoma stem cell behavior. J Biomed Mater Res B Appl Biomater, 103(3), 618-628. doi:10.1002/jbm.b.33214

2. Mak, K. M., & Mei, R. (2017). Basement Membrane Type IV Collagen and Laminin: An Overview of Their Biology and Value as Fibrosis Biomarkers of Liver Disease. Anat Rec (Hoboken), 300(8), 1371-1390. doi:10.1002/ar.23567

3. Choi, S., Yu, J., Park, A., Dubon, M. J., Do, J., Kim, Y., . . . Park, K. S. (2019). BMP-4 enhances epithelial mesenchymal transition and cancer stem cell properties of breast cancer cells via Notch signaling. Sci Rep, 9(1), 11724. doi:10.1038/s41598-019-48190-5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Laminin
Biologically Active P
NBP1-78301
Laminin Biologically Active Protein
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Applications: Func, ICC/IF, NULL

Publications for Laminin Biologically Active Protein (NBP1-78301)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: ICC/IF.


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Review for Laminin Biologically Active Protein (NBP1-78301) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-78301:
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IF
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Images Ratings Applications Species Date Details
Immunofluorescence Laminin NBP1-78301
Enlarge 		5
reviewed by:
Verified Customer
 IF Human 11/04/2014
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Summary

ApplicationImmunofluorescence
Sample TestedAdhesion of stem cell and HUVEC cells on single cell arrays
SpeciesHuman
LotNBP1-78301 Lot A

Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Laminin Biologically Active Protein (NBP1-78301). (Showing 1 - 5 of 5 FAQs).

  1. What research areas can this product be used in?
    • All Laminin products can be used in: Angiogenesis, Apoptosis, Cancer, Cell Biology, Cellular Markers, Extracellular Matrix, Neuroscience, Signal Transduction, Tumor Suppressors.
  2. What is the theoretical molecular weight for your Laminin antibodies?
    • The theoretical molecular weight for Laminin antibodies is 337 kDa.
  3. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
    • If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.
  4. I'm looking for a basement membrane marker, can you suggest a Laminin antibody that can be used for this purpose?
    • The following Laminin antibodies are Basement Membrane markers: NB120-17107, NB300-144, NB600-883, and NBP1-51759.
  5. I have seen the product sheet of NBP1-78301 and specifications of this product does not have the sequence information. My purpose of this protein to use it for phage display tech to select the antibodies and for other function assay like western etc., I also would like to know 1) Is this is full length Laminin protein 2) Is this has sequence for Laminin 2 alpha G ?
    • NBP1-78301 is the whole Laminin protein and is a highly pure form of laminin-1 from mouse tumor. Please note that this product can be used for functional assay as well as IF and it is not recommended to use it for western blot.

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Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.
Could Laminin be Used to Treat Duchenne Muscular Dystrophy?
Duchenne muscular dystrophy (DMD) is a severe muscle wasting condition, causing disability and early death. There is currently no cure or adequate treatment for DMD, but pioneering research indicates that injection of a laminin protein may prevent (or...  Read full blog post.
Using the Laminin Antibody in Angiogenesis Research
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Recent Reviews

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Immunofluorescence Laminin NBP1-78301
Enlarge
 Verified Customer
11/04/2014
Application: IF
Species: Human
read this review

Bioinformatics

Gene Symbol LAMA1