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![Western Blot: SNF5 Antibody (3E10) [H00006598-M01] - SMARCB1 monoclonal antibody (M01), clone 3E10. Analysis of SMARCB1 expression in Raw 264.7.](http://images.novusbio.com/fullsize/SNF5-Antibody-3E10-Western-Blot-H00006598-M01-img0012.jpg "Western Blot: SNF5 Antibody (3E10) [H00006598-M01] - SMARCB1 monoclonal antibody (M01), clone 3E10. Analysis of SMARCB1 expression in Raw 264.7.")
![Immunohistochemistry-Paraffin: SNF5 Antibody (3E10) [H00006598-M01] - Analysis of monoclonal antibody to SMARCB1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/SNF5-Antibody-3E10-Immunohistochemistry-Paraffin-H00006598-M01-img0008.jpg)
![Western Blot: SNF5 Antibody (3E10) [H00006598-M01] - SMARCB1 monoclonal antibody (M01), clone 3E10 Analysis of SMARCB1 expression in PC-12.](http://images.novusbio.com/fullsize/SNF5-Antibody-3E10-Western-Blot-H00006598-M01-img0010.jpg)
![Western Blot: SNF5 Antibody (3E10) [H00006598-M01] - SMARCB1 monoclonal antibody (M01), clone 3E10. Analysis of SMARCB1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/SNF5-Antibody-3E10-Western-Blot-H00006598-M01-img0011.jpg)
![Western Blot: SNF5 Antibody (3E10) [H00006598-M01] - SMARCB1 monoclonal antibody (M01), clone 3E10. Analysis of SMARCB1 expression in NIH/3T3.](http://images.novusbio.com/fullsize/SNF5-Antibody-3E10-Western-Blot-H00006598-M01-img0013.jpg)
![ELISA: SNF5 Antibody (3E10) [H00006598-M01] - Detection limit for recombinant GST tagged SMARCB1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SNF5-Antibody-3E10-ELISA-H00006598-M01-img0014.jpg)
![Western Blot: SNF5 Antibody [NBP1-90013] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/SNF5-Antibody-Western-Blot-NBP1-90013-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: SNF5 Antibody [NBP1-90013] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: SNF5 Antibody [NBP1-90013] - Analysis using Anti-SMARCB1 antibody NBP1-90013 (A) shows similar pattern to independent antibody NBP1-90014 (B).](http://images.novusbio.com/fullsize/SNF5-Antibody-Western-Blot-NBP1-90013-img0009.jpg)
![Immunohistochemistry-Paraffin: SNF5 Antibody [NBP1-90013] - Staining of human liver shows very weak positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/SNF5-Antibody-Immunohistochemistry-Paraffin-NBP1-90013-img0013.jpg)
![Western Blot: SNF5 Antibody [NBP1-90013] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/SNF5-Antibody-Western-Blot-NBP1-90013-img0008.jpg)
![Immunohistochemistry-Paraffin: SNF5 Antibody [NBP1-90013] - Staining of human tonsil shows nuclear positivity.](http://images.novusbio.com/fullsize/SNF5-Antibody-Immunohistochemistry-Paraffin-NBP1-90013-img0007.jpg)
![Immunohistochemistry-Paraffin: SNF5 Antibody [NBP1-90013] - Staining of human cerebellum shows strong nuclear positivity in Purkinje cells and in cells in granular and molecular layer.](http://images.novusbio.com/fullsize/SNF5-Antibody-Immunohistochemistry-Paraffin-NBP1-90013-img0011.jpg)
![Immunohistochemistry-Paraffin: SNF5 Antibody [NBP1-90013] - Staining of human duodenum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SNF5-Antibody-Immunohistochemistry-Paraffin-NBP1-90013-img0012.jpg)
![Western Blot: SNF5 Antibody [NBP1-90014] - Analysis using Anti-SMARCB1 antibody NBP1-90014 (A) shows similar pattern to independent antibody NBP1-90013 (B).](http://images.novusbio.com/fullsize/SNF5-Antibody-Western-Blot-NBP1-90014-img0012.jpg "<b>Independent Antibodies Validation. </b>Western Blot: SNF5 Antibody [NBP1-90014] - Analysis using Anti-SMARCB1 antibody NBP1-90014 (A) shows similar pattern to independent antibody NBP1-90013 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: SNF5 Antibody [NBP1-90014] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SNF5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90014-img0006.jpg)
![Immunohistochemistry-Paraffin: SNF5 Antibody [NBP1-90014] - Staining of human tonsil shows nuclear positivity.](http://images.novusbio.com/fullsize/SNF5-Antibody-Immunohistochemistry-Paraffin-NBP1-90014-img0011.jpg)
![Western Blot: SNF5 Antibody [NBP1-90014] - Analysis in human cell line HEK 293.](http://images.novusbio.com/fullsize/SNF5-Antibody-Western-Blot-NBP1-90014-img0008.jpg)
![Western Blot: SNF5 Antibody [NBP1-90014] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/SNF5-Antibody-Western-Blot-NBP1-90014-img0009.jpg)
![Western Blot: SNF5 Antibody [NB100-57525] - Detection of Human and Mouse SMARCB1/SNF5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCB1/SNF5 antibody NB100-57525 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SNF5-Antibody-Western-Blot-NB100-57525-img0013.jpg "<b>Biological Strategies Validation. </b>Western Blot: SNF5 Antibody [NB100-57525] - Detection of Human and Mouse SMARCB1/SNF5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCB1/SNF5 antibody NB100-57525 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: SNF5 Antibody [NB100-57525] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- SMARCB1/SNF5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SNF5-Antibody-Immunohistochemistry-NB100-57525-img0011.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: SNF5 Antibody [NB100-57525] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. NB100-57525 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SMARCB1/SNF5 was also immunoprecipitated by another rabbit anti-SMARCB1/SNF5 antibody, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/SNF5-Antibody-Western-Blot-NB100-57525-img0002.jpg)
![Immunohistochemistry: SNF5 Antibody [NB100-57525] - Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti- SMARCB1/SNF5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SNF5-Antibody-Immunohistochemistry-NB100-57525-img0010.jpg)
![Immunoprecipitation: SNF5 Antibody [NB100-57525] - Detection of human SMARCB1/SNF5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCB1/SNF5 antibody NB100-57525 (lot NB100-57525-2) used for IP at 6 ug per reaction. SMARCB1/SNF5 was also immunoprecipitated by a previous lot of this antibody NB100-57525-1. For blotting immunoprecipitated SMARCB1/SNF5, NB100-57525 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SNF5-Antibody-Immunoprecipitation-NB100-57525-img0014.jpg)
![Western Blot: SNF5 Antibody (6X6T8) [NBP3-16159] - Western blot analysis of extracts of various cell lines, using SMARCB1/SNF5 Rabbit mAb (NBP3-16159) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/SNF5-Antibody-6X6T8-Western-Blot-NBP3-16159-img0004.jpg "Western Blot: SNF5 Antibody (6X6T8) [NBP3-16159] - Western blot analysis of extracts of various cell lines, using SMARCB1/SNF5 Rabbit mAb (NBP3-16159) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunocytochemistry/Immunofluorescence: SNF5 Antibody (6X6T8) [NBP3-16159] - Immunofluorescence analysis of U-2 OS cells using SMARCB1/SNF5 Rabbit mAb (NBP3-16159) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SNF5-Antibody-6X6T8-Immunocytochemistry-Immunofluorescence-NBP3-16159-img0003.jpg)
![Western Blot: SNF5 Antibody (6X6T8) [NBP3-16159] - Western blot analysis of extracts of various cell lines, using SMARCB1/SNF5 Rabbit mAb (NBP3-16159) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/SNF5-Antibody-6X6T8-Western-Blot-NBP3-16159-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SNF5 Antibody (6X6T8) [NBP3-16159] - Immunofluorescence analysis of NIH-3T3 cells using SMARCB1/SNF5 Rabbit mAb (NBP3-16159) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SNF5-Antibody-6X6T8-Immunocytochemistry-Immunofluorescence-NBP3-16159-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: SNF5 Antibody (6X6T8) [NBP3-16159] - Western blot analysis of extracts of 293T cells, using SMARCB1/SNF5 antibody (NBP3-16159) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/SNF5-Antibody-6X6T8-Knockout-Validated-NBP3-16159-img0001.jpg)
![Western Blot: SNF5 Antibody (3E3) [H00006598-M02] - SMARCB1 monoclonal antibody (M02), clone 3E3. Analysis of SMARCB1 expression in Raw 264.7.](http://images.novusbio.com/fullsize/SNF5-Antibody-3E3-Western-Blot-H00006598-M02-img0004.jpg "Western Blot: SNF5 Antibody (3E3) [H00006598-M02] - SMARCB1 monoclonal antibody (M02), clone 3E3. Analysis of SMARCB1 expression in Raw 264.7.")
![Western Blot: SNF5 Antibody (3E3) [H00006598-M02] - SMARCB1 monoclonal antibody (M02), clone 3E3. Analysis of SMARCB1 expression in PC-12.](http://images.novusbio.com/fullsize/SNF5-Antibody-3E3-Western-Blot-H00006598-M02-img0002.jpg)
![Western Blot: SNF5 Antibody (3E3) [H00006598-M02] - SMARCB1 monoclonal antibody (M02), clone 3E3 Analysis of SMARCB1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/SNF5-Antibody-3E3-Western-Blot-H00006598-M02-img0003.jpg)
![Western Blot: SNF5 Antibody (3E3) [H00006598-M02] - SMARCB1 monoclonal antibody (M02), clone 3E3. Analysis of SMARCB1 expression in NIH/3T3.](http://images.novusbio.com/fullsize/SNF5-Antibody-3E3-Western-Blot-H00006598-M02-img0005.jpg)
![Immunohistochemistry-Paraffin: SNF5 Antibody (SMARCB1/3984) [NBP3-13774] - Formalin-fixed, paraffin-embedded human epithelioid sarcoma stained with SNF5 antibody (SMARCB1/3984).](http://images.novusbio.com/fullsize/SNF5-Antibody-SMARCB1-3984-Immunohistochemistry-Paraffin-NBP3-13774-img0003.jpg "Immunohistochemistry-Paraffin: SNF5 Antibody (SMARCB1/3984) [NBP3-13774] - Formalin-fixed, paraffin-embedded human epithelioid sarcoma stained with SNF5 antibody (SMARCB1/3984).")
![Immunohistochemistry-Paraffin: SNF5 Antibody (SMARCB1/3984) [NBP3-13774] - Formalin-fixed, paraffin-embedded human testis stained with SNF5 antibody (SMARCB1/3984) at 2ug/ml. Inset: PBS instead of primary antibody, secondary negative control.](http://images.novusbio.com/fullsize/SNF5-Antibody-SMARCB1-3984-Immunohistochemistry-Paraffin-NBP3-13774-img0001.jpg)
![Immunohistochemistry-Paraffin: SNF5 Antibody (SMARCB1/3984) [NBP3-13774] - Formalin-fixed, paraffin-embedded human epithelioid sarcoma stained with SNF5 antibody (SMARCB1/3984).](http://images.novusbio.com/fullsize/SNF5-Antibody-SMARCB1-3984-Immunohistochemistry-Paraffin-NBP3-13774-img0002.jpg)
![Protein Array: SNF5 Antibody (SMARCB1/3984) [NBP3-13774] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SNF5 Antibody (SMARCB1/3984).](http://images.novusbio.com/fullsize/SNF5-Antibody-SMARCB1-3984-Protein-Array-NBP3-13774-img0005.jpg)
