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![Western Blot: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Analysis of DYKDDDDK tagged protein demonstrating that the rat monoclonal DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] is 10-15 fold more sensitive than Sigma's M2 mouse ANTI-FLAG M2 antibody.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-L5-Western-Blot-NBP1-06712-img0010.jpg "Western Blot: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Analysis of DYKDDDDK tagged protein demonstrating that the rat monoclonal DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] is 10-15 fold more sensitive than Sigma's M2 mouse ANTI-FLAG M2 antibody.")
![Western Blot: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Analysis of DYKDDDDK tagged SFO-p41 protein with DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712]. Observed molecular weight ~46.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-L5-Western-Blot-NBP1-06712-img0005.jpg)
![Western Blot: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Analysis of DYKDDDDK tagged AIFm2 protein (A), DYKDDDDK tagged IREB2 protein (B), and vector control (C) using DYKDDDDK epitope tag antibody (L5) [NBP1-06712] at 2 ug/ml.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-L5-Western-Blot-NBP1-06712-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Analysis of HeLa cells transfected with a FLAG encoded construct (top) or untransfected (bottom), using DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712]. Image from verified customer review.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-L5-Immunocytochemistry-Immunofluorescence-NBP1-06712-img0009.jpg)
![Immunohistochemistry: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Third instar larva of the genotype [hs-Flp; nrv2-Gal4/MCFO-2]. flp expression of the multicolor FlpOut construct was induced by 1 h 37C heat shock during first instar stage. Larvae were stained for HA (green), V5 (red), and FLAG (blue). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32901033/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-L5-Immunohistochemistry-NBP1-06712-img0011.jpg)
![Western Blot: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Detection of DDK in SUSD3 overexpression lysate using [NBP1-06712] followed by goat anti-rat IgG HRP conjugated secondary antibody (cat.# NB7115). Image from verified customer review.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-L5-Western-Blot-NBP1-06712-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Rat Monoclonal DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Analysis using DyLight 488 conjugated antibody (NBP1-06712G). Normal mouse prostate cells overexpressed with Flag-tagged construct (Green: Flag). Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-06712_rat-monoclonal-dykddddk-epitope-tag-antibody-l5-immunocytochemistry-immunofluorescence-792023163813..jpg)
![Immunohistochemistry: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Generation of a wrapping glia driver.Confocal projection of larval filet preparations of the genotypes indicated. Representative images are shown taken from >10 animals analyzed for each genotype. a Third instar larva with the genotype [nrv2-stGFP]. Broad GFP expression is detected in the CNS. Note the restricted expression in the peripheral nervous system which corresponds to the wrapping glia (arrowheads). b Third instar larva of the genotype [hs-Flp; nrv2-Gal4/MCFO-2]. flp expression of the multicolor FlpOut construct was induced by 1 h 37 °C heat shock during first instar stage. Larvae were stained for HA (green), V5 (red), & FLAG (blue). c Same animal as in (a). Expression of stRed is observed only in the CNS & no expression is found in the wrapping glia. d–f Overlay of nrv2-GFP (green) & 90C03 > dsRed (red) expression. Note the complete overlap of dsRed (e) & GFP expression in the CNS (f). g Young third instar larva with the genotype [hs-Flp; nrv2-Gal4/MCFO-2; 90C03-Gal80]. flp expression was induced by 1 h 37 °C heat shock during first instar stage. Larvae were stained for HA (green), V5 (red), & FLAG (blue). h Living third instar larva of the genotype [nrv2-Gal4, UAS-CD8GFP; 90C03-Gal80/90C03-Gal80]. Note strong expression at the anterior tip of the larva. Scale bars are 250 µm (a–c, g, h) & 100 µm (d–f). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32901033), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06712_rat-monoclonal-dykddddk-epitope-tag-antibody-l5-310202416182637.jpg)
![Western Blot: E-Tag Epitope Tag Antibody [NB600-518] - Detection of E-Tag-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-E-Tag antibody NB600-518 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/E-Tag-Epitope-Tag-Antibody-Western-Blot-NB600-518-img0004.jpg "Western Blot: E-Tag Epitope Tag Antibody [NB600-518] - Detection of E-Tag-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-E-Tag antibody NB600-518 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: S-Tag Epitope Tag Antibody [NB600-511] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).](http://images.novusbio.com/fullsize/S-Tag-Epitope-Tag-Antibody-Western-Blot-NB600-511-img0002.jpg "Western Blot: S-Tag Epitope Tag Antibody [NB600-511] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).")
![Western Blot: S-Tag Epitope Tag Antibody [NB600-529] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-S-Tag antibody NB600-529 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/S-Tag-Epitope-Tag-Antibody-Western-Blot-NB600-529-img0004.jpg "Western Blot: S-Tag Epitope Tag Antibody [NB600-529] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-S-Tag antibody NB600-529 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: S-Tag Epitope Tag Antibody [NB600-529] - Analysis using the HRP conjugate of NB600-529. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).](http://images.novusbio.com/fullsize/S-Tag-Epitope-Tag-Antibody-Western-Blot-NB600-529-img0003.jpg)
![Western Blot: E-Tag Epitope Tag Antibody [NBP3-05892] - A: Etag-GFERh(C) transfected 293 cell lysate at 2ugLane B: his-Etag-GFER(C) transfected 293 cell lysate at 2ugLane C: his-GFER-Etag(C) transfected 293 cell lysate at 2ugLane D: Etag-GSTT2h(C) transfected 293 cell lysate at 10ugLane E: his-GSTT2-Etag(C) transfected 293 cell lysate at 10ugLane F: his-Etag-GSTT2(C) transfected 293 cell lysate at 20ugLane G: Nagetive control at 10ugSecondaryDylight 800-labeled Antibody To Rabbit IgG (H+L) at 1/5000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.](http://images.novusbio.com/fullsize/E-Tag-Epitope-Tag-Antibody-Western-Blot-NBP3-05892-img0003.jpg "Western Blot: E-Tag Epitope Tag Antibody [NBP3-05892] - A: Etag-GFERh(C) transfected 293 cell lysate at 2ugLane B: his-Etag-GFER(C) transfected 293 cell lysate at 2ugLane C: his-GFER-Etag(C) transfected 293 cell lysate at 2ugLane D: Etag-GSTT2h(C) transfected 293 cell lysate at 10ugLane E: his-GSTT2-Etag(C) transfected 293 cell lysate at 10ugLane F: his-Etag-GSTT2(C) transfected 293 cell lysate at 20ugLane G: Nagetive control at 10ugSecondaryDylight 800-labeled Antibody To Rabbit IgG (H+L) at 1/5000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.")
![Immunocytochemistry/Immunofluorescence: E-Tag Epitope Tag Antibody [NBP3-05892] - Staining of E-Tag in Hela cells, transfected with pSTEP2-Etag-GFERh (Figure A), pSTEP2-his-Etag-GFER (Figure B), pSTEP2-his-GFER-Etag (Figure C), pSTEP2-Etag-GSTT2h (Figure D),pSTEP2-his-Etag-GSTT2 (Figure E) and pSTEP2-his-GSTT2-Etag (Figure F). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-E-Tag polyclonal antibody at 37? 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/E-Tag-Epitope-Tag-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05892-img0001.jpg)
![Immunoprecipitation: E-Tag Epitope Tag Antibody [NBP3-05892] - Lane A:0.5 mg E tag-1M-125D-His Whole Cell LysateLane B:0.5 mg His-E tag-1M-125D Whole Cell LysateLane C:0.5 mg His-1M-125D-E tag Whole Cell LysateLane D:0.5 mg E tag-1M-244P-His Whole Cell LysateLane E:0.5 mg Psetp2 Whole Cell Lysate2 uL anti-E-tag rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-E-tag rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.](http://images.novusbio.com/fullsize/E-Tag-Epitope-Tag-Antibody-Immunoprecipitation-NBP3-05892-img0002.jpg)
![Western Blot: E-Tag Epitope Tag Antibody [NB600-527] - Analysis using the HRP conjugate of NB600-527. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.04 ug/ml (1:25,000).](http://images.novusbio.com/fullsize/E-Tag-Epitope-Tag-Antibody-Western-Blot-NB600-527-img0004.jpg "Western Blot: E-Tag Epitope Tag Antibody [NB600-527] - Analysis using the HRP conjugate of NB600-527. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.04 ug/ml (1:25,000).")
![Western Blot: E-Tag Epitope Tag Antibody [NB600-527] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.04 ug/ml (1:25,000).](http://images.novusbio.com/fullsize/E-Tag-Epitope-Tag-Antibody-Western-Blot-NB600-527-img0002.jpg)
![Western Blot: S-Tag Epitope Tag Antibody (727) [NBP2-43817] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of S-tagged protein expressing 293T cells 12 % SDS-PAGE S Tag antibody [727] dilution: 1:5000.](http://images.novusbio.com/fullsize/S-Tag-Epitope-Tag-Antibody-727-Western-Blot-NBP2-43817-img0001.jpg "Western Blot: S-Tag Epitope Tag Antibody (727) [NBP2-43817] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of S-tagged protein expressing 293T cells 12 % SDS-PAGE S Tag antibody [727] dilution: 1:5000.")
![Immunocytochemistry/Immunofluorescence: S-Tag Epitope Tag Antibody (727) [NBP2-43817] - S Tag antibody detects S Tag protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: S Tag stained by S Tag antibody diluted at 1:2000. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/S-Tag-Epitope-Tag-Antibody-727-Immunocytochemistry-Immunofluorescence-NBP2-43817-img0002.jpg)
![Western Blot: E-Tag Epitope Tag Antibody (B2-E2) [NBP2-67081] - Western blot analysis of E-Tag Epitope Tag on E-tagged recombinant protein using anti-E-Tag Epitope Tag antibody at 1/20000 dilution.](http://images.novusbio.com/fullsize/E-Tag-Epitope-Tag-Antibody-B2-E2-Western-Blot-NBP2-67081-img0002.jpg "Western Blot: E-Tag Epitope Tag Antibody (B2-E2) [NBP2-67081] - Western blot analysis of E-Tag Epitope Tag on E-tagged recombinant protein using anti-E-Tag Epitope Tag antibody at 1/20000 dilution.")
![Western Blot: E-Tag Epitope Tag Antibody (B2-E2) [NBP2-67081] - Analysis of E tag on E-tagged recombinant protein using anti-E tag antibody at 1/20000 dilution.](http://images.novusbio.com/fullsize/E-Tag-Epitope-Tag-Antibody-B2-E2-Western-Blot-NBP2-67081-img0001.jpg)
![Western Blot: S-Tag Epitope Tag Antibody (247) [NBP2-43726] - Analysis of A. 30 ug 293T whole cell lysate/eztract B. 30 ug whole cell lysate/extract of S-tagged protein expressing 293T cells 12 % SDS-PAGE S Tag antibody dilution: 1:5000.](http://images.novusbio.com/fullsize/S-Tag-Epitope-Tag-Antibody-247-Western-Blot-NBP2-43726-img0002.jpg "Western Blot: S-Tag Epitope Tag Antibody (247) [NBP2-43726] - Analysis of A. 30 ug 293T whole cell lysate/eztract B. 30 ug whole cell lysate/extract of S-tagged protein expressing 293T cells 12 % SDS-PAGE S Tag antibody dilution: 1:5000.")
![Western Blot: S-Tag Epitope Tag Antibody (247) [NBP2-43726] - Analysis of 400 ug cell lysate/extract of S-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of S-fused protein by 2 ug of S tag antibody 12% SDS-PAGE The immunoprecipitated S-fused protein was detected by S tag antibody diluted at 1:1000. EasyBlot anti-mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/S-Tag-Epitope-Tag-Antibody-247-Western-Blot-NBP2-43726-img0001.jpg)
![Western Blot: S-Tag Epitope Tag Antibody (G10-C8-R) [NBP3-32923] - Western blot analysis of S-Tag Epitope Tag on S tag recombinant protein with Mouse anti-S-Tag Epitope Tag antibody (NBP3-32923) at 1/1,000 dilution. Lysates/proteins at 50 ng/Lane. Exposure time: 25 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32923) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32923_mouse-s-tag-epitope-tag-mab-g10-c8-r-288202412442986.jpg "Western Blot: S-Tag Epitope Tag Antibody (G10-C8-R) [NBP3-32923] - Western blot analysis of S-Tag Epitope Tag on S tag recombinant protein with Mouse anti-S-Tag Epitope Tag antibody (NBP3-32923) at 1/1,000 dilution. Lysates/proteins at 50 ng/Lane. Exposure time: 25 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32923) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")