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E-Tag Epitope Tag Antibody [CoraFluor™ 1]

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Product Details

Summary
Reactivity ETSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clonality
Polyclonal
Host
Goat
Conjugate
CoraFluor 1

Order Details

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E-Tag Epitope Tag Antibody [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Goats were immunized with E-Tag (GAPVPYPDPLEPR) conjugated to KLH.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for E-Tag Epitope Tag Antibody [CoraFluor™ 1]

  • E epitope tag
  • GAPVPYPDPLEPR epitope tag
  • GAPVPYPDPLEPR tag

Background

The E-Tag is a popular epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized E-tag epitope represents the peptide sequence GAPVPYPDPLEPR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional E-Tag Epitope Tag Products

Array NB600-518CL1

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Contact Information

Product PDFs

Calculators

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