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S-Tag Epitope Tag Antibody [HRP]

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Western Blot: S-Tag Epitope Tag Antibody [HRP] [NB600-510] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).

Product Details

Summary
Reactivity ETSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC (-)
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Concentration
1.0 mg/ml

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S-Tag Epitope Tag Antibody [HRP] Summary

Immunogen
Goats were immunized with S-Tag (KETAAAKFERQHMDS) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • ELISA 1:10000 -1:100000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:500
  • Western Blot 1:1000-1:30000
Application Notes
Immunohistochemistry Not applicable
Readout System

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS and 0.2% BSA
Preservative
0.05% Pro-Clean 400
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for S-Tag Epitope Tag Antibody [HRP]

  • KETAAAKFERQHMDS
  • S 7 Epitope tag

Background

The S-Tag epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized S-Tag epitope represents the amino acid sequence KETAAAKFERQHMDS derived from pancreatic ribonuclease A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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