| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6219-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB62191-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6219-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03809-20ul | |||
![Western Blot: TRIM21/SSA1 Antibody [NBP1-33548] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SSA1 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TRIM21-SSA1-Antibody-Western-Blot-NBP1-33548-img0015.jpg "Western Blot: TRIM21/SSA1 Antibody [NBP1-33548] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SSA1 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: TRIM21/SSA1 Antibody [NBP1-33548] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/TRIM21-SSA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33548-img0007.jpg)
![Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-33548] - DLD-1 xenograft , using SSA1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/TRIM21-SSA1-Antibody-Immunohistochemistry-Paraffin-NBP1-33548-img0008.jpg)
![Immunoprecipitation: TRIM21/SSA1 Antibody [NBP1-33548] - Immunoprecipitation of SSA1 protein from 293T whole cell extracts using 5 ug of SSA1 antibody [C1C3]. Western blot analysis was performed using SSA1 antibody [C1C3] EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/TRIM21-SSA1-Antibody-Immunoprecipitation-NBP1-33548-img0011.jpg)
![Western Blot: TRIM21/SSA1 Antibody [NBP1-87122] - Analysis in control (vector only transfected HEK293T lysate) and TRIM21 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/TRIM21-SSA1-Antibody-Western-Blot-NBP1-87122-img0015.jpg "Western Blot: TRIM21/SSA1 Antibody [NBP1-87122] - Analysis in control (vector only transfected HEK293T lysate) and TRIM21 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: TRIM21/SSA1 Antibody [NBP1-87122] - Staining of human cell line HUVEC TERT2 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TRIM21-SSA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87122-img0006.jpg)
![Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-87122] - Staining of human tonsil shows moderate to strong cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/TRIM21-SSA1-Antibody-Immunohistochemistry-Paraffin-NBP1-87122-img0012.jpg)
![Western Blot: TRIM21/SSA1 Antibody [NBP1-87122] - Analysis in human cell line CAPAN-2.](http://images.novusbio.com/fullsize/TRIM21-SSA1-Antibody-Western-Blot-NBP1-87122-img0014.jpg)
![Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-87122] - Staining of human kidney shows moderate cytoplasmic and stronger nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/TRIM21-SSA1-Antibody-Immunohistochemistry-Paraffin-NBP1-87122-img0009.jpg)
![Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-87122] - Staining of human placenta shows strong cytoplasmic immunoreactivity in monocytes, as well as moderate positivity in trophoblast.](http://images.novusbio.com/fullsize/TRIM21-SSA1-Antibody-Immunohistochemistry-Paraffin-NBP1-87122-img0010.jpg)
![Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-87122] - Staining of human skeletal muscle shows only weak positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/TRIM21-SSA1-Antibody-Immunohistochemistry-Paraffin-NBP1-87122-img0011.jpg)
![Immunohistochemistry: TRIM21/SSA1 Antibody (PSH02-56) [NBP3-33051] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-TRIM21/SSA1 antibody (NBP3-33051) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33051) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33051_rabbit-trim21-ssa1-mab-psh02-56-298202413585512.jpg "Immunohistochemistry: TRIM21/SSA1 Antibody (PSH02-56) [NBP3-33051] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-TRIM21/SSA1 antibody (NBP3-33051) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33051) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: TRIM21/SSA1 Antibody (PSH02-56) [NBP3-33051] - Immunocytochemistry analysis of U-2 OS cells labeling TRIM21/SSA1 with Rabbit anti-TRIM21/SSA1 antibody (NBP3-33051) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TRIM21/SSA1 antibody (NBP3-33051) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33051_rabbit-trim21-ssa1-mab-psh02-56-29202410484849.jpg)
![Flow Cytometry: TRIM21/SSA1 Antibody (PSH02-56) [NBP3-33051] - Flow cytometric analysis of U-2 OS cells labeling TRIM21/SSA1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33051, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33051_rabbit-trim21-ssa1-mab-psh02-56-392024884618.jpg)
![Western Blot: TRIM21/SSA1 Antibody (PSH02-56) [NBP3-33051] - Western blot analysis of TRIM21/SSA1 on different lysates with Rabbit anti-TRIM21/SSA1 antibody (NBP3-33051) at 1/10,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 10ng/mL IFN-alpha for 16 hours cell lysate Lane 3: A549 cell lysate Lane 4: 293T cell lysate Lane 5: U-2 OS cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 54 kDa Observed band size: 50 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33051_rabbit-trim21-ssa1-mab-psh02-56-3920248485026.jpg)
![Knockout Validated: TRIM21/SSA1 Antibody [NBP3-03809] - Analysis of extracts from normal (control) and TRIM21 knockout (KO) A-549 cells, using TRIM21/SSA1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/TRIM21-SSA1-Antibody-Knockout-Validated-NBP3-03809-img0001.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: TRIM21/SSA1 Antibody [NBP3-03809] - Analysis of extracts from normal (control) and TRIM21 knockout (KO) A-549 cells, using TRIM21/SSA1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody (2C9) [H00006737-M01] - Analysis of monoclonal antibody to TRIM21 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/TRIM21-SSA1-Antibody-2C9-Immunohistochemistry-Paraffin-H00006737-M01-img0002.jpg "Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody (2C9) [H00006737-M01] - Analysis of monoclonal antibody to TRIM21 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.")
![ELISA: TRIM21/SSA1 Antibody (2C9) [H00006737-M01] - Detection limit for recombinant GST tagged TRIM21 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TRIM21-SSA1-Antibody-2C9-ELISA-H00006737-M01-img0004.jpg)
![Western Blot: TRIM21/SSA1 Antibody [NB100-1209] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human TRIM21 with C-terminal MYC tag probed (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B.](http://images.novusbio.com/fullsize/TRIM21-SSA1-Antibody-Western-Blot-NB100-1209-img0002.jpg "Western Blot: TRIM21/SSA1 Antibody [NB100-1209] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human TRIM21 with C-terminal MYC tag probed (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B.")
![SDS-Page: Recombinant Human TRIM21/SSA1 Protein [H00006737-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00006737-Q01-1.jpg "SDS-Page: Recombinant Human TRIM21/SSA1 Protein [H00006737-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Recombinant Human TRIM21/SSA1 Protein [H00006737-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00006737-P01-1.jpg "Recombinant Human TRIM21/SSA1 Protein [H00006737-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: TRIM21/SSA1 Antibody [NBP1-40338] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-SSA1used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. SSA1 was also immunoprecipitated by rabbit anti-SSA1 antibody NBP1-37009, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/TRIM21-SSA1-Antibody-Western-Blot-NBP1-40338-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: TRIM21/SSA1 Antibody [NBP1-40338] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-SSA1used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. SSA1 was also immunoprecipitated by rabbit anti-SSA1 antibody NBP1-37009, which recognizes an upstream epitope.")