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XPF Antibody (OTI4E11) [DyLight 755]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI4E11
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 755

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74883AF350
Alexa Fluor 405
NBP2-74883AF405
Alexa Fluor 488
NBP2-74883AF488
Alexa Fluor 532
NBP2-74883AF532
Alexa Fluor 594
NBP2-74883AF594
Alexa Fluor 647
NBP2-74883AF647
Alexa Fluor 700
NBP2-74883AF700
Alexa Fluor 750
NBP2-74883AF750
Allophycocyanin
NBP2-74883APC
Allophycocyanin/Cy7
NBP2-74883APCCY7
Biotin
NBP2-74883B
CoraFluor 1
NBP2-74883CL1
DyLight 350
NBP2-74883UV
DyLight 405
NBP2-74883V
DyLight 488
NBP2-74883G
DyLight 550
NBP2-74883R
DyLight 594
NBP2-74883DL594
DyLight 650
NBP2-74883C
DyLight 680
NBP2-74883FR
FITC
NBP2-74883F
HRP
NBP2-74883H
Janelia Fluor 525
NBP2-74883JF525
Janelia Fluor 549
NBP2-74883JF549
Janelia Fluor 585
NBP2-74883JF585
Janelia Fluor 635
NBP2-74883JF635
Janelia Fluor 646
NBP2-74883JF646
Janelia Fluor 669
NBP2-74883JF669
PE
NBP2-74883PE
PE/Atto594
NBP2-74883PEATT594
PE/Cy5.5
NBP2-74883PECY55
PE/Cy7
NBP2-74883PECY7
PerCP
NBP2-74883PCP
Unconjugated
NBP2-01020
mFluor Violet 450 SE
NBP2-74883MFV450
mFluor Violet 500 SE
NBP2-74883MFV500
mFluor Violet 610 SE
NBP2-74883MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-01020
Azide and BSA Free
NBP2-74883
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XPF Antibody (OTI4E11) [DyLight 755] Summary

Immunogen
Full length recombinant protein of human XPF (NP_005227) produced in HEK293 cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ERCC4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for XPF Antibody (OTI4E11) [DyLight 755]

  • DNA excision repair protein ERCC-4
  • DNA repair protein complementing XP-F cells
  • EC 3.1
  • ERCC11
  • ERCC4
  • excision repair cross-complementing rodent repair deficiency, complementationgroup 4
  • xeroderma pigmentosum, complementation group F
  • XPF
  • XPFcomplementing defective, in Chinese hamster

Background

XPF/ERCC4 is suggested to play a role in the repair of DNA double-strand breaks (DSB), homologous recombination, and gene conversion via single-strand annealing (SSA). XPF/ERCC4 is an endonuclease that incises 5-prime DNA. Defects in XPF/ERCC4 cause xeroderma pigmentosum VI (XP6) an autosomal recessive disease characterized by hypersensitivity to sunlight and a predisposition to skin cancer as well as neurological abnormalities. Defects in XPF/ERCC4 are also responsible for XFE progeroid syndrome, a syndrome characterized by dwarfism, cachexia, and microcephaly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ERCC4