![Western Blot: XLF Antibody (3D6) [NBP2-03275] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XLF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XLF.](http://images.novusbio.com/fullsize/XLF-Antibody-3D6-Western-Blot-NBP2-03275-img0027.jpg "Western Blot: XLF Antibody (3D6) [NBP2-03275] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XLF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XLF.")
| Reactivity | Hu, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI3D6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.52 mg/ml |
| Immunogen | Full length human recombinant protein of human NHEJ1 (NP_079058) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NHEJ1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 33.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.52 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for XLF Antibody (NBP2-03275)Find related products by research area.
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Ku70: The DNA's Mr. Fix-it Ku70, known by several synonyms including X-ray repair cross-complementing, 5'-deoxyribose-5-phosphate lyase Ku70 protein 6, 70 kDa subunit of Ku antigen, XRCC6, and G22P1, is a 70 kDa protein that was shown to be involved in multiple cellular pathway... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: XLF Antibody (3D6) [NBP2-03275] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY XLF.](http://images.novusbio.com/fullsize/XLF-Antibody-3D6-Immunocytochemistry-Immunofluorescence-NBP2-03275-img0016.jpg "Immunocytochemistry/Immunofluorescence: XLF Antibody (3D6) [NBP2-03275] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY XLF.")
![Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Human prostate tissue using anti-XLF mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XLF-Antibody-3D6-Immunohistochemistry-Paraffin-NBP2-03275-img0026.jpg "Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Human prostate tissue using anti-XLF mouse monoclonal antibody.")
![Flow Cytometry: XLF Antibody (3D6) [NBP2-03275] - Analysis of Hela cells, using anti-XLF antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/XLF-Antibody-3D6-Flow-Cytometry-NBP2-03275-img0015.jpg "Flow Cytometry: XLF Antibody (3D6) [NBP2-03275] - Analysis of Hela cells, using anti-XLF antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Western Blot: XLF Antibody (3D6) [NBP2-03275] Analysis of extracts (35ug) from 9 different cell lines by using anti-XLF monoclonal antibody.](http://images.novusbio.com/fullsize/XLF-Antibody-3D6-Western-Blot-NBP2-03275-img0014.jpg "Western Blot: XLF Antibody (3D6) [NBP2-03275] Analysis of extracts (35ug) from 9 different cell lines by using anti-XLF monoclonal antibody.")
![Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-XLF mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XLF-Antibody-3D6-Immunohistochemistry-Paraffin-NBP2-03275-img0017.jpg "Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-XLF mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-XLF mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XLF-Antibody-3D6-Immunohistochemistry-Paraffin-NBP2-03275-img0018.jpg "Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-XLF mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-XLF mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XLF-Antibody-3D6-Immunohistochemistry-Paraffin-NBP2-03275-img0019.jpg "Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-XLF mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-XLF mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XLF-Antibody-3D6-Immunohistochemistry-Paraffin-NBP2-03275-img0020.jpg "Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-XLF mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Human endometrium tissue using anti-XLF mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XLF-Antibody-3D6-Immunohistochemistry-Paraffin-NBP2-03275-img0021.jpg "Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Human endometrium tissue using anti-XLF mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Human Kidney tissue using anti-XLF mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XLF-Antibody-3D6-Immunohistochemistry-Paraffin-NBP2-03275-img0022.jpg "Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Human Kidney tissue using anti-XLF mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Human liver tissue using anti-XLF mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XLF-Antibody-3D6-Immunohistochemistry-Paraffin-NBP2-03275-img0023.jpg "Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Human liver tissue using anti-XLF mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Human lymph node tissue using anti-XLF mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XLF-Antibody-3D6-Immunohistochemistry-Paraffin-NBP2-03275-img0024.jpg "Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Human lymph node tissue using anti-XLF mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Human lymphoma tissue using anti-XLF mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XLF-Antibody-3D6-Immunohistochemistry-Paraffin-NBP2-03275-img0025.jpg "Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Human lymphoma tissue using anti-XLF mouse monoclonal antibody.")
![Western Blot Histone H2AX [p Ser139] Antibody [Unconjugated]](https://images.novusbio.com/images2/Histone_H2AX_AF2288_Western_Blot_5182.jpg)
![Simple Western Histone H2AX [p Ser139] Antibody [Unconjugated]](https://images.novusbio.com/images2/16395.jpg)
![Immunocytochemistry Histone H2AX [p Ser139] Antibody [Unconjugated]](https://images.novusbio.com/images2/Histone_H2AX_AF2288_Immunocytochemistry__Immunofluorescence_16920.jpg)
![Western Blot Chk2 [p Thr68] Antibody [Unconjugated]](https://images.novusbio.com/images2/Chk2_AF1626_Western_Blot_5130.jpg)
![Immunocytochemistry Chk2 [p Thr68] Antibody [Unconjugated]](https://images.novusbio.com/images2/Chk2_AF1626_Immunocytochemistry__Immunofluorescence_18847.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")