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Wnt-4 Antibody [Biotin]

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Product Details

Summary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

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Wnt-4 Antibody [Biotin] Summary

Specificity
Detects mouse Wnt-4 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
WNT4
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Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Wnt-4 is a 38‑42 kDa member of the Wnt family of secreted glycoproteins, which act as short-range signaling molecules via Frizzled receptors and a cascade of intracellular signals in vertebrate embryogenesis (1‑2). Mouse Wnt-4 is synthesized as a 351 amino acid (aa) precursor with a 22 aa signal sequence and a 329 aa mature chain. The mature chain contains two potential sites for N-linked glycosylation. Relative to other members of the Wnt family, Wnt-4 contains 83 conserved aa, including 21 cysteines (1). Mature mouse Wnt-4 shares 99% aa sequence identity with mature human and rat Wnt-4. Wnt-4 has been shown to play a critical role in the development of the reproductive system and in the formation of the kidneys, adrenals, pituitary gland, and mammary tissues (3‑6). In the development of the reproductive system, Wnt-4 expression is down-regulated in the developing gonad after E11.5, although it persists in the developing ovary (2, 6). Targeted deletion of Wnt-4 results in masculinization of XX mice, with rudimentary development of the masculine internal (Wolffian) ducts and degeneration of the female (Mullerian) reproductive tract (2, 6). In addition to its involvement in urogenital development, Wnt-4 is also expressed in the perichondrium of the long bones (7), and promotes osteoblast differentiation (8). Wnt-4 may also be associated with abnormal proliferation in human breast tissue (9). In humans, mutations in Wnt-4 are the cause of SERKAL syndrome, a syndrome consisting of female to male sex reversal, renal, adrenal, and lung dysgenesis, and developmental defects (3), and Rokitansky-Kuster-Hauser syndrome, which is characterized by utero-vaginal atresia in otherwise phenotypically normal females with normal 46, XX karyotype (10).

  1. Gavin, B.J. et al. (1990) Genes Dev. 4:2319.
  2. Jordan, B.K. et al. (2001) Am. J. Hum. Genet. 68:1102.
  3. Mandel, H. et al. (2008) Am. J. Hum. Genet. 82:39.
  4. Bernard, P. and V.R. Harley (2007) Int. J. Biochem. Cell Biol. 39:31.
  5. Kuulasmaa, T. et al. (2008) Horm. Metab. Res. 40:668.
  6. Vainio, S. et al. (1999) Nature 397:405.
  7. Gao, X. (2004) Genes Dev. 18:2404.
  8. Chang, J. et al. (2007) J. Biol. Chem. 282:30938.
  9. Huguet, E.L. et al. (1994) Cancer Res. 54:2615.
  10. Philibert, P. et al. (2008) J. Clin. Endocrinol. Metab. 93:895.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol WNT4