VPS26A Antibody (CL2287)

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: VPS26A Antibody (CL2287) [NBP2-36754] - Staining in human testis and skeletal muscle tissues. Corresponding VPS26A RNA-seq data are presented for the same ...read more
Genetic Strategies: Western Blot: VPS26A Antibody (CL2287) [NBP2-36754] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, ...read more
Immunocytochemistry/ Immunofluorescence: VPS26A Antibody (CL2287) [NBP2-36754] - Staining in U251 cell line showing distinct staining of endosomes in green. Microtubule-staining and nuclear probes are visualized in red ...read more
Western Blot: VPS26A Antibody (CL2287) [NBP2-36754] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: VPS26A Antibody (CL2287) [NBP2-36754] - Staining in HeLa cell line showing distinct staining of endosomes in green. Microtubule-staining and nuclear probes are visualized in red ...read more
Immunocytochemistry/ Immunofluorescence: VPS26A Antibody (CL2287) [NBP2-36754] - Staining of human cell line A431 showing distinct staining of endosomes in green. Microtubule-staining and nuclear probes are visualized ...read more
Immunocytochemistry/ Immunofluorescence: VPS26A Antibody (CL2287) [NBP2-36754] - Staining in MCF7 cell line showing distinct staining of endosomes in green. Microtubule-staining and nuclear probes are visualized in red ...read more
Immunocytochemistry/ Immunofluorescence: VPS26A Antibody (CL2287) [NBP2-36754] - Staining in U2OS cell line with Anti-VSP26A monoclonal antibody, showing distinct staining of endosomes in green. Microtubule- and nuclear ...read more
Immunohistochemistry-Paraffin: VPS26A Antibody (CL2287) [NBP2-36754] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: VPS26A Antibody (CL2287) [NBP2-36754] - Staining of human fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: VPS26A Antibody (CL2287) [NBP2-36754] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: VPS26A Antibody (CL2287) [NBP2-36754] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clone
CL2287
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

VPS26A Antibody (CL2287) Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:LRKQRTNFHQRFESPESQASAEQPEM
Predicted Species
Mouse (96%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
VPS26A
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
VPS26A Recombinant Protein Antigen (NBP2-36754PEP)
Publications
Read Publications using
NBP2-36754 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for VPS26A Antibody (CL2287)

  • FLJ12930
  • HB58
  • Hbeta58
  • hVPS26
  • PEP8A
  • vacuolar protein sorting 26 (yeast homolog)
  • vacuolar protein sorting 26 homolog A (S. pombe)
  • vacuolar protein sorting 26 homolog A (yeast)
  • vacuolar protein sorting-associated protein 26A
  • Vesicle protein sorting 26A
  • VPS26vacuolar protein sorting 26 (yeast)

Background

VPS26 belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VPS26A Antibody (NBP2-36754)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol VPS26A
Uniprot