Recombinant Human Uteroglobin/SCGB1A1 Protein Summary
Description |
A disulfide-linked homodimeric protein containing two 70 amino acid polypeptide chains corresponding to Uteroglobin/SCGB1A1 Source: E. coli Uniprot ID: P11684 Amino Acid Sequence: EICPSFQRVI ETLLMDTPSS YEAAMELFSP DQDMREAGAQ LKKLVDTLPQ KPRESIIKLM EKIAQSSLCN |
Details of Functionality |
Uteroglobin/SCGB1A1 protein is fully biologically active when compared to standard. The ED50 as determined by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cells is less than 5.0 ug/ml, corresponding to a specific activity of > 200 IU/mg |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
SCGB1A1 |
Purity |
> 98 % pure by SDS-PAGE and HPLC |
Endotoxin Note |
Less than 0.1 EU/ug of Uteroglobin/SCGB1A1 as determined by LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
15.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Buffer |
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
Preservative |
No Preservative |
Concentration |
LYOPH |
Purity |
> 98 % pure by SDS-PAGE and HPLC |
Reconstitution Instructions |
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
Notes
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Alternate Names for Recombinant Human Uteroglobin/SCGB1A1 Protein
Background
Uteroglobin, also known as secretoglobin family 1A member 1 (SCGB1A1), is encoded by thek SCGB1A1 gene. It is the founding protein of the secretoglobin family, whose members are small, secreted, disulfide-bridged dimeric proteins found in mammals only. Uteroglobin is specifically expressed in clara cells in the lungs. It is multifunctional and found in various tissues in various names such as: uteroglobin (UG, UGB), uteroglobin-like antigen (UGL), blastokinin, clara cell secretory protein (CCSP), clara cell 16 kD protein (17 in rat/mice), clara Cell-Specific 10 kD protein (CC10), human protein 1, urine protein 1 (UP-1), polychlorinated biphenyl-binding protein (PCB-BP), human clara cell phospholipid-binding protein (hCCPBP), secretoglobin 1A member 1 (SCGB1A1). Its putative functions include immunomodulation, progesterone binding (weak in some animals, especially weak in humans), phospholipase A2 inhibitor etc. Human uteroglobin shares 50 % ~ 60 % amino acid sequence identity with murine, rat, equine and bovine uteroglobins.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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