Reactivity | MuSpecies Glossary |
Applications | Flow |
Clone | 237104 |
Clonality | Monoclonal |
Host | Rat |
Conjugate | Alexa Fluor 405 |
Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
Immunogen | BaF3 mouse pro-B cell line transfected with mouse ULBP‑1 /MULT-1 |
Specificity | Detects mouse ULBP‑1 /MULT-1. |
Source | N/A |
Isotype | IgG2a |
Clonality | Monoclonal |
Host | Rat |
Gene | ULBP1 |
Purity Statement | Protein A or G purified from hybridoma culture supernatant |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells. |
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Publications |
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Storage | Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
Buffer | Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide. |
Preservative | 0.09% Sodium Azide |
Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
ULBP-1, also known as MULT-1 (mouse UL16-binding protein-like Transcript 1), is a 53 kDa, MHC Class I-like molecule that belongs to the mouse family of NKG2D ligands (1‑4). It is a type I transmembrane glycoprotein that is synthesized as a 334 amino acid (aa) precursor. It contains a 25 aa signal sequence, a 186 aa extracellular region, a 19 aa transmembrane segment and a 104 aa cytoplasmic tail (2). The extracellular region contains an alpha 1 and alpha 2 like domain with two intrachain disulfide bonds. ULBP-1 is distantly related to other human and mouse NKG2D ligands, and more distantly related to the MHC class I proteins (3). Unlike most NKG2D ligands, transcripts for ULBP-1 have been detected in a wide variety of mouse tissues and tumor cells lines (3). The receptor for ULBP-1 is NKG2D, a 35 kDa C-type lectin that is found on mouse NK cells, activated CD8+ T cells, epidermal gamma δ T cells, and activated macrophages (1, 5, 6, 7). Recombinant ULBP-1 protein binds to NKG2D with high affinity (KD = 6 nM) (2). Although an activating receptor, general cellular responses to NKG2D ligation depend upon the isoform of NKG2D and the cell type (5). Exposure to immobilized ULBP-1 or ULBP-1-transfected cells elicits IFN-gamma production by NK cells (3). Ectopic expression of ULBP-1 on the RMA mouse tumor cell line leads to tumor rejection in syngeneic mice (3).
Publication using FAB2588V | Applications | Species |
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WN Jin, K Shi, W He, JH Sun, L Van Kaer, FD Shi, Q Liu Neuroblast senescence in the aged brain augments natural killer cell cytotoxicity leading to impaired neurogenesis and cognition Nature Neuroscience, 2020-11-30;0(0):. 2020-11-30 [PMID: 33257875] (Flow Cytometry, Mouse) | Flow Cytometry | Mouse |
Secondary Antibodies |
Isotype Controls |
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Gene Symbol | ULBP1 |