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Recombinant Human UBE2K/E2-25K His (N-Term) Protein

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SDS-Page: Human UBE2K/E2-25K His Protein [NBP2-35096]
SDS-Page: Human UBE2K/E2-25K His Protein [NBP2-35096]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Format
Carrier-Free

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Catalog# & Formulation Size Price
Carrier-Free
NBP2-35096-1mg
Carrier-Free
NBP2-35096-100ug
Carrier-Free
NBP2-35096-500ug
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Recombinant Human UBE2K/E2-25K His (N-Term) Protein Summary

Description
A single non-glycosylated polypeptide chain containing 200 amino acids corresponding to UBE2K/E2-25K and 8 amino acid vector sequence including 6x His tag at N-terminus Source: E. coli

Uniprot ID: P61086

Amino Acid Sequence: MHHHHHHAMA NIAVQRIKRE FKEVLKSEET SKNQIKVDLV DENFTELRGE IAGPPDTPYE GGRYQLEIKI PETYPFNPPK VRFITKIWHP NISSVTGAIC LDILKDQWAA AMTLRTVLLS LQALLAAAEP DDPQDAVVAN QYKQNPEMFK QTARLWAHVY AGAPVSSPEY TKKIENLCAM GFDRNAVIVA LSSKSWDVET ATELLLSN

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
UBE2K
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of UBE2K/E2-25K as determined by LAL method.

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
23.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 8.0, with 100 mM NaCl, 10 % Glycerol, 1 mM DTT.
Preservative
5% Trehalose
Purity
>95%, by SDS-PAGE and HPLC
Reconstitution Instructions
Please contact technical support for detailed reconstitution instructions.

Alternate Names for Recombinant Human UBE2K/E2-25K His (N-Term) Protein

  • E2(25K)
  • E225K
  • E2-25K
  • EC 6.3.2.19
  • Hip2
  • HIP-2
  • huntingtin interacting protein 2
  • Huntingtin-interacting protein 2
  • HYPG
  • LIG
  • UbcH1
  • UBE2K
  • UBE2K-25K
  • ubiquitin carrier protein
  • ubiquitin-conjugating enzyme E2 K
  • Ubiquitin-conjugating enzyme E2(25K)
  • Ubiquitin-conjugating enzyme E2-25 kDa
  • Ubiquitin-conjugating enzyme E2-25K
  • ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast)
  • ubiquitin-protein ligase

Background

Ubiquitin-conjugating enzyme E2 K (UBE2K) is a protein that in humans is encoded by the UBE2K gene. The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. It binds selectively to a large region at the N terminus of huntingtin. This interaction is not influenced by the length of the huntingtin polyglutamine tract. This protein has been implicated in the degradation of huntingtin and suppression of apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol UBE2K
Uniprot