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UbcH5b/UBE2D2 Antibody (OTI2C2)

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Western Blot: UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2D2.
Western Blot: UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2D2.
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (OTI2C2) [NBP2-46270] - UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120 ...read more
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI2C2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74732AF350
Alexa Fluor 405
NBP2-74732AF405
Alexa Fluor 488
NBP2-74732AF488
Alexa Fluor 532
NBP2-74732AF532
Alexa Fluor 594
NBP2-74732AF594
Alexa Fluor 647
NBP2-74732AF647
Alexa Fluor 700
NBP2-74732AF700
Alexa Fluor 750
NBP2-74732AF750
Biotin
NBP2-74732B
CoraFluor 1
NBP2-74732CL1
DyLight 350
NBP2-74732UV
DyLight 405
NBP2-74732V
DyLight 488
NBP2-74732G
DyLight 550
NBP2-74732R
DyLight 594
NBP2-74732DL594
DyLight 650
NBP2-74732C
DyLight 680
NBP2-74732FR
DyLight 755
NBP2-74732IR
FITC
NBP2-74732F
HRP
NBP2-74732H
Janelia Fluor 525
NBP2-74732JF525
Janelia Fluor 549
NBP2-74732JF549
Janelia Fluor 585
NBP2-74732JF585
Janelia Fluor 635
NBP2-74732JF635
Janelia Fluor 646
NBP2-74732JF646
Janelia Fluor 669
NBP2-74732JF669
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-74732
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UbcH5b/UBE2D2 Antibody (OTI2C2) Summary

Immunogen
Full length human recombinant protein of human UBE2D2 (NP_003330) produced in E.coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
UBE2D2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
16.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UbcH5b/UBE2D2 Antibody (OTI2C2)

  • E2(17)KB2
  • EC 6.3.2.19
  • PUBC1
  • UBC4/5
  • UBC4ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5)
  • UBC5B
  • UBCH4
  • UbcH5b
  • UBE2D2
  • Ubiquitin carrier protein D2
  • ubiquitin-conjugating enzyme E2 D2
  • Ubiquitin-conjugating enzyme E2(17)KB 2
  • Ubiquitin-conjugating enzyme E2-17 kDa 2
  • ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast)
  • Ubiquitin-protein ligase D2

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol UBE2D2