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UbcH2/UBE2H Antibody [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-00048AF350
Alexa Fluor 405
NBP3-00048AF405
Alexa Fluor 488
NBP3-00048AF488
Alexa Fluor 532
NBP3-00048AF532
Alexa Fluor 594
NBP3-00048AF594
Alexa Fluor 647
NBP3-00048AF647
Alexa Fluor 700
NBP3-00048AF700
Alexa Fluor 750
NBP3-00048AF750
Allophycocyanin
NBP3-00048APC
Biotin
NBP3-00048B
DyLight 350
NBP3-00048UV
DyLight 405
NBP3-00048V
DyLight 488
NBP3-00048G
DyLight 550
NBP3-00048R
DyLight 594
NBP3-00048DL594
DyLight 650
NBP3-00048C
DyLight 680
NBP3-00048FR
DyLight 755
NBP3-00048IR
FITC
NBP3-00048F
HRP
NBP3-00048H
Janelia Fluor 525
NBP3-00048JF525
Janelia Fluor 549
NBP3-00048JF549
Janelia Fluor 585
NBP3-00048JF585
Janelia Fluor 635
NBP3-00048JF635
Janelia Fluor 646
NBP3-00048JF646
Janelia Fluor 669
NBP3-00048JF669
PE
NBP3-00048PE
PerCP
NBP3-00048PCP
Unconjugated
NBP3-00048-100ul
mFluor Violet 450 SE
NBP3-00048MFV450
mFluor Violet 500 SE
NBP3-00048MFV500
mFluor Violet 610 SE
NBP3-00048MFV610
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UbcH2/UBE2H Antibody [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Produced in rabbits immunized with purified, recombinant Human UbcH2/UBE2H (Accession#: P62256; Met1-Leu183)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBE2H
Purity
Antigen and protein A Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Antigen and protein A Affinity-purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for UbcH2/UBE2H Antibody [CoraFluor™ 1]

  • E2-14K
  • E2-20K
  • EC 6.3.2.19
  • UBC8
  • UBC8E2-20K
  • UBCH
  • UbcH2
  • UBE2H
  • Ubiquitin carrier protein H
  • ubiquitin-conjugating enzyme E2 H
  • Ubiquitin-conjugating enzyme E2-20K
  • ubiquitin-conjugating enzyme E2H (homologous to yeast UBC8)
  • ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast)
  • Ubiquitin-protein ligase H

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol UBE2H