![Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Tubulin alpha-1B Antibody (114) detects Tubulin alpha-1B protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin stained by Tubulin alpha-1B Antibody (114) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Immunocytochemistry-Immunofluorescence-NBP2-43837-img0020.jpg "Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Tubulin alpha-1B Antibody (114) detects Tubulin alpha-1B protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin stained by Tubulin alpha-1B Antibody (114) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.")
| Reactivity | Hu, Mu, Rt, Dr, Fi, Gt, Ze, Po, Bv, Pm, Rb, RM, XpSpecies Glossary |
| Applications | WB, ICC/IF, IHC, Func |
| Clone | 114 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Tubulin alpha-1B. The exact sequence is proprietary. |
| Predicted Species | Porcine (100%), Bovine (100%), Rabbit (100%), Rhesus Macaque (100%), Xenopus (95%), Primate (99%). Backed by our 100% Guarantee. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TUBA1B |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol (pH7) |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Tubulin alpha-1B Antibody (NBP2-43837)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | TUBA1B |
![Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Paraffin-embedded mouse colon. Red: alpha Tubulin antibody [114] diluted at 1:500. Green: beta Catenin antibody [N1N2-2] (NBP1-32239) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Immunohistochemistry-Paraffin-NBP2-43837-img0011.jpg "Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Paraffin-embedded mouse colon. Red: alpha Tubulin antibody [114] diluted at 1:500. Green: beta Catenin antibody [N1N2-2] (NBP1-32239) diluted at 1:500. Blue: Hoechst 33342 staining.")
![Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug NIH-3T3 whole cell lysate/extract C. 30 ug mouse brain lysate/extract D. 30 ug PC-12 whole cell lysate/extract E. 30 ug rat brain lysate/extract F. 30 ug drosophila lysae/extract 10 % SDS-PAGE alpha Tubulin antibody [114] dilution: 1:5000.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Western-Blot-NBP2-43837-img0004.jpg "Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug NIH-3T3 whole cell lysate/extract C. 30 ug mouse brain lysate/extract D. 30 ug PC-12 whole cell lysate/extract E. 30 ug rat brain lysate/extract F. 30 ug drosophila lysae/extract 10 % SDS-PAGE alpha Tubulin antibody [114] dilution: 1:5000.")
![Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of A. 30 ug zebrafish eye lysate/extract 10 % SDS-PAGE alpha Tubulin antibody [114] dilution: 1:1000.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Western-Blot-NBP2-43837-img0005.jpg "Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of A. 30 ug zebrafish eye lysate/extract 10 % SDS-PAGE alpha Tubulin antibody [114] dilution: 1:1000.")
![Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of varied amount of 293T Whole cell extracts was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody at a dilution of 1:10000.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Western-Blot-NBP2-43837-img0006.jpg "Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of varied amount of 293T Whole cell extracts was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody at a dilution of 1:10000.")
![Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [114] diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Western-Blot-NBP2-43837-img0013.jpg "Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [114] diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [114] diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Western-Blot-NBP2-43837-img0015.jpg "Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [114] diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of HeLa cells were fixed in -20? 100% methanol for 5 min. Green: TUBA1B protein stained by TUBA1B antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Immunocytochemistry-Immunofluorescence-NBP2-43837-img0001.jpg "Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of HeLa cells were fixed in -20? 100% methanol for 5 min. Green: TUBA1B protein stained by TUBA1B antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of Green: alpha Tubulin antibody [114] diluted at 1:200. The signal was developed using goat anti-mouse IgG antibody (Dylight488). Blue: Nuclear staining with Hoechst 33342.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Immunocytochemistry-Immunofluorescence-NBP2-43837-img0009.jpg "Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of Green: alpha Tubulin antibody [114] diluted at 1:200. The signal was developed using goat anti-mouse IgG antibody (Dylight488). Blue: Nuclear staining with Hoechst 33342.")
![Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin protein stained by alpha Tubulin antibody [114] diluted at 1:250. Red: NeuN, stained by NeuN antibody diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Immunocytochemistry-Immunofluorescence-NBP2-43837-img0017.jpg "Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin protein stained by alpha Tubulin antibody [114] diluted at 1:250. Red: NeuN, stained by NeuN antibody diluted at 1:250. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of human cervix. alpha Tubulin antibody [114] diluted at 1:500.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Immunohistochemistry-Paraffin-NBP2-43837-img0002.jpg "Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of human cervix. alpha Tubulin antibody [114] diluted at 1:500.")
![Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of human lung cancer. alpha Tubulin antibody [114] diluted at 1:500.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Immunohistochemistry-Paraffin-NBP2-43837-img0003.jpg "Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of human lung cancer. alpha Tubulin antibody [114] diluted at 1:500.")
![Tumorsphere: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B Antibody (114) diluted at 1:10000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Tumorsphere-NBP2-43837-img0019.jpg "Tumorsphere: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B Antibody (114) diluted at 1:10000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B antibody [GT114] (NBP2-43837) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43837_mouse-monoclonal-tubulin-alpha-1b-antibody-114-910202420131278.jpg "Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B antibody [GT114] (NBP2-43837) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
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