![SDS-Page: Recombinant Human Troponin I type 2 (fast skeletal) Protein [NBP2-22893]](http://images.novusbio.com/fullsize/Troponin-I-type-2-fast-skeletal-Protein-SDS-Page-NBP2-22893-img0001.jpg "SDS-Page: Recombinant Human Troponin I type 2 (fast skeletal) Protein [NBP2-22893]")
| Reactivity | HuSpecies Glossary |
| Applications | PAGE |
| Concentration | 0.25 mg/ml |
| Description | A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-182 of Human Troponin I type 2 (fast skeletal) Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGDEEKRNRA ITARRQHLKS VMLQIAATEL EKEESRREAE KQNYLAEHCP PLHIPGSMSE VQELCKQLHA KIDAAEEEKY DMEVRVQKTS KELEDMNQKL FDLRGKFKRP PLRRVRMSAD AMLKALLGSK HKVCMDLRAN LKQVKKEDTE KERDLRDVGD WRKNIEEKSG MEGRKKMFES ES |
| Source | E. coli |
| Protein/Peptide Type | Recombinant Protein |
| Gene | TNNI2 |
| Purity | >90%, by SDS-PAGE |
| Dilutions |
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| Application Notes | Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays. |
| Theoretical MW | 23.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 20 mM Tris-HCl buffer (pH 8.0), 2M UREA, 20% glycerol, 0.2 M NaCl |
| Preservative | No Preservative |
| Concentration | 0.25 mg/ml |
| Purity | >90%, by SDS-PAGE |
Research Areas for Troponin I type 2 (fast skeletal) Recombinant Protein (NBP2-22893)Find related products by research area.
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Troponin I Type 2 - I stay with fast-twitch skeletal muscles only The protein Troponin I is a component of the heteromeric protein complex responsible for regulating both skeletal and cardiac muscle contraction. Troponin complex is made up of three parts: troponin I (TnI), troponin T (TnT), and troponin C (TnC). Eac... Read full blog post. |
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| Gene Symbol | TNNI2 |
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![Western Blot: Troponin I type 2 (fast skeletal) Antibody [NBP2-26200] - Porcine muscle RIPA extract, 15 ug per well. Primary antibody 1:1000. Blocker 5% rabbit serum. Samples: 1) longissimus dorsi (fast twitch); 2) flexor digitorum (slow twitch); 3) vastus lateralis (fast twitch); diaphragm (mixed); 5) cardiac. This image was submitted via customer review.](https://images.novusbio.com/images/Troponin-I-type-2-fast-skeletal-Antibody-Western-Blot-NBP2-26200-img0004.jpg "Western Blot: Troponin I type 2 (fast skeletal) Antibody [NBP2-26200] - Porcine muscle RIPA extract, 15 ug per well. Primary antibody 1:1000. Blocker 5% rabbit serum. Samples: 1) longissimus dorsi (fast twitch); 2) flexor digitorum (slow twitch); 3) vastus lateralis (fast twitch); diaphragm (mixed); 5) cardiac. This image was submitted via customer review.")