![Western Blot: Troponin I type 2 (fast skeletal) Antibody [NBP2-26200] - Porcine muscle RIPA extract, 15 ug per well. Primary antibody 1:1000. Blocker 5% rabbit serum. Samples: 1) longissimus dorsi (fast twitch); 2) flexor digitorum (slow twitch); 3) vastus lateralis (fast twitch); diaphragm (mixed); 5) cardiac. This image was submitted via customer review.](http://images.novusbio.com/fullsize/Troponin-I-type-2-fast-skeletal-Antibody-Western-Blot-NBP2-26200-img0004.jpg "Western Blot: Troponin I type 2 (fast skeletal) Antibody [NBP2-26200] - Porcine muscle RIPA extract, 15 ug per well. Primary antibody 1:1000. Blocker 5% rabbit serum. Samples: 1) longissimus dorsi (fast twitch); 2) flexor digitorum (slow twitch); 3) vastus lateralis (fast twitch); diaphragm (mixed); 5) cardiac. This image was submitted via customer review.")
| Reactivity | Hu, Mu, Rt, Po, CaSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence KRNRAITARRQHLKS-C corresponding to N-Terminus according to NP_001139313.1. |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_003273.1; NP_001139313.1). Reported variants represent identical protein: NP_001139301.1, NP_003273.1. |
| Predicted Species | Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | TNNI2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | WB: Approx. 25 kDa band observed in human, mouse and rat skeletal muscle lysates (calculated MW of 21.3 kDa band according to NP_003273.1). |
|
| Reviewed Applications |
|
|
| Publications |
|
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP2-26200 | Applications | Species |
|---|---|---|
| Moran CM, Garriock RJ, Miller MK et al. Expression of the fast twitch troponin complex, fTnT, fTnI and fTnC, in vascular smooth muscle. Cell Motil Cytoskeleton 2008-08-01 [PMID: 18548613] |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Gail Otulakowski |
WB | Pig | 02/13/2018 |
Summary
|
Secondary Antibodies |
Isotype Controls |
Research Areas for Troponin I type 2 (fast skeletal) Antibody (NBP2-26200)Find related products by research area.
|
|
Troponin I Type 2 - I stay with fast-twitch skeletal muscles only The protein Troponin I is a component of the heteromeric protein complex responsible for regulating both skeletal and cardiac muscle contraction. Troponin complex is made up of three parts: troponin I (TnI), troponin T (TnT), and troponin C (TnC). Eac... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: Troponin I type 2 (fast skeletal) Antibody [NBP2-26200] - (0.03ug/ml) staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp2-26200_goat-polyclonal-troponin-i-type-2-fast-skeletal-antibody-187202311342914.jpg "Western Blot: Troponin I type 2 (fast skeletal) Antibody [NBP2-26200] - (0.03ug/ml) staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
