![Western Blot: Troponin I type 2 (fast skeletal) Overexpression Lysate (Adult Normal) [NBP2-11337] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Troponin I type 2 (fast skeletal).](http://images.novusbio.com/fullsize/Troponin-I-type-2-fast-skeletal-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11337-img0001.jpg "Western Blot: Troponin I type 2 (fast skeletal) Overexpression Lysate (Adult Normal) [NBP2-11337] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Troponin I type 2 (fast skeletal).")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Description | Troponin I type 2 (fast skeletal) Transient Overexpression LysateExpression Host: HEK293T Plasmid: RC227419 Accession#: NM_001145829 Protein Tag: C-MYC/DDK You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT. |
| Gene | TNNI2 |
| Dilutions |
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| Application Notes | This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct. Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading. |
| Theoretical MW | 21.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | RIPA buffer |
Research Areas for Troponin I type 2 (fast skeletal) Lysate (NBP2-11337)Find related products by research area.
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Troponin I Type 2 - I stay with fast-twitch skeletal muscles only The protein Troponin I is a component of the heteromeric protein complex responsible for regulating both skeletal and cardiac muscle contraction. Troponin complex is made up of three parts: troponin I (TnI), troponin T (TnT), and troponin C (TnC). Eac... Read full blog post. |
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| Gene Symbol | TNNI2 |
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![Western Blot: Troponin I type 2 (fast skeletal) Antibody [NBP2-26200] - Porcine muscle RIPA extract, 15 ug per well. Primary antibody 1:1000. Blocker 5% rabbit serum. Samples: 1) longissimus dorsi (fast twitch); 2) flexor digitorum (slow twitch); 3) vastus lateralis (fast twitch); diaphragm (mixed); 5) cardiac. This image was submitted via customer review.](https://images.novusbio.com/images/Troponin-I-type-2-fast-skeletal-Antibody-Western-Blot-NBP2-26200-img0004.jpg "Western Blot: Troponin I type 2 (fast skeletal) Antibody [NBP2-26200] - Porcine muscle RIPA extract, 15 ug per well. Primary antibody 1:1000. Blocker 5% rabbit serum. Samples: 1) longissimus dorsi (fast twitch); 2) flexor digitorum (slow twitch); 3) vastus lateralis (fast twitch); diaphragm (mixed); 5) cardiac. This image was submitted via customer review.")